2025
Spatial transcriptomics in inflammatory skin diseases using GeoMx digital spatial profiling: a practical guide for applications in dermatology
Cho C, Haddadi N, Kidacki M, Woodard G, Shakiba S, Yıldız-Altay Ü, Richmond J, Vesely M. Spatial transcriptomics in inflammatory skin diseases using GeoMx digital spatial profiling: a practical guide for applications in dermatology. JID Innovations 2025, 5: 100317. DOI: 10.1016/j.xjidi.2024.100317.Peer-Reviewed Original ResearchDigital spatial profilingInflammatory skin diseaseGeoMx Digital Spatial ProfilerSkin diseasesDiscoid lupus erythematosusNanoString GeoMx Digital Spatial ProfilingImmune milieuImmune microenvironmentLichen planusLupus erythematosusSingle cell RNA sequencingAberrant inflammationCell RNA sequencingSkin disease pathogenesisDisease pathogenesisManufacturer's guidelinesIndicators of diseaseDiseaseSpatial transcriptomics platformSkinTissue processing
2024
207 Spatial transcriptomic profiling non-small cell lung cancer reveals potential drivers of CTL exclusion and dysfunction, and identifies novel predictive biomarkers for checkpoint blockade therapy
Cho C, Lopez-Giraldez F, Huang B, He J, Woodard G, Badri T, Kidacki M, Vesely M, Wang G, Ofori-Ntiamoah G, Ng E, Chen L. 207 Spatial transcriptomic profiling non-small cell lung cancer reveals potential drivers of CTL exclusion and dysfunction, and identifies novel predictive biomarkers for checkpoint blockade therapy. 2024, a236-a236. DOI: 10.1136/jitc-2024-sitc2024.0207.Peer-Reviewed Original Research
2023
Immune Inhibitory Molecule PD-1 Homolog (VISTA) Colocalizes with CD11b Myeloid Cells in Melanoma and Is Associated with Poor Outcomes
Vesely M, Kidacki M, Gaule P, Gupta S, Chan N, Han X, Yeung J, Chen L. Immune Inhibitory Molecule PD-1 Homolog (VISTA) Colocalizes with CD11b Myeloid Cells in Melanoma and Is Associated with Poor Outcomes. Journal Of Investigative Dermatology 2023, 144: 106-115.e4. PMID: 37562584, DOI: 10.1016/j.jid.2023.07.008.Peer-Reviewed Original ResearchConceptsCD11b myeloid cellsImmune inhibitory moleculesPD-L1 expressionVISTA expressionMyeloid cellsFuture potential therapeutic targetsPD-L1/B7Tumor microenvironmentInhibitory moleculesMultiplexed quantitative immunofluorescencePrimary cutaneous melanomaImmunosuppressive tumor microenvironmentNegative prognostic biomarkerCurrent clinical trialsPotential therapeutic targetCause mortalityCritical cell typesPD-L1Poor outcomeTreatment of cancerMelanoma recurrenceCutaneous melanomaClinical trialsPrognostic biomarkerT cells
2021
Iatrogenic calcinosis cutis from extravasated phosphate-containing solution treated with topical sodium thiosulfate
Xie CB, Kidacki M, Ring N, Panse G, Leventhal JS. Iatrogenic calcinosis cutis from extravasated phosphate-containing solution treated with topical sodium thiosulfate. JAAD Case Reports 2021, 17: 31-33. PMID: 34692964, PMCID: PMC8517711, DOI: 10.1016/j.jdcr.2021.09.013.Peer-Reviewed Case Reports and Technical Notes
2020
Twist2 is NFkB-responsive when p120-catenin is inactivated and EGFR is overexpressed in esophageal keratinocytes
Lehman H, Kidacki M, Stairs D. Twist2 is NFkB-responsive when p120-catenin is inactivated and EGFR is overexpressed in esophageal keratinocytes. Scientific Reports 2020, 10: 18829. PMID: 33139779, PMCID: PMC7608670, DOI: 10.1038/s41598-020-75866-0.Peer-Reviewed Original ResearchConceptsEsophageal squamous cell carcinomaCancer typesYear of diagnosisSquamous cell carcinomaEsophageal keratinocytesOverexpression of EGFRHuman esophageal keratinocytesAggressive cell typesFatal cancer typePoor prognosisCell carcinomaTwist2 expressionEGFR overexpressionEGFREMT-inducing geneNFkBResponsive targetsTwist2Paucity of researchCell typesPresent studyKeratinocytesP120-cateninOverexpressionComplete loss
2019
E‐cadherin and p120ctn protein expression are lost in hidradenitis suppurativa lesions
Nelson AM, Cong Z, Gettle SL, Longenecker AL, Kidacki M, Kirby JS, Adams DR, Stairs DB, Danby FW. E‐cadherin and p120ctn protein expression are lost in hidradenitis suppurativa lesions. Experimental Dermatology 2019, 28: 867-871. PMID: 31107992, DOI: 10.1111/exd.13973.Peer-Reviewed Original ResearchConceptsProtein expressionHS lesional skinPathogenesis of HSInflammatory skin diseaseJunction proteinsHidradenitis suppurativa lesionsInflammatory skin conditionNon-lesional skinSevere skin lesionsE-cadherinAdherens junction proteinsE-cadherin expressionHurley stageAtopic dermatitisLesional skinDesmosome sizeHS pathogenesisHS skinAcne vulgarisHS patientsSkin lesionsPilosebaceous unitSkin conditionsSkin diseasesHealthy skin1028 Deciphering the mechanism of action of isotretinoin in acne patients using next gene sequencing technologies
Thiboutot D, Kidacki M, Sarfo A, Schneider A, Albert I, Longenecker A, Cong Z, Nelson A. 1028 Deciphering the mechanism of action of isotretinoin in acne patients using next gene sequencing technologies. Journal Of Investigative Dermatology 2019, 139: s178. DOI: 10.1016/j.jid.2019.03.1104.Peer-Reviewed Original Research‘Invasive proliferative gelatinous mass’ of hidradenitis suppurativa contains distinct inflammatory components
Kidacki M, Cong Z, Flamm A, Helm K, Danby FW, Nelson AM. ‘Invasive proliferative gelatinous mass’ of hidradenitis suppurativa contains distinct inflammatory components. British Journal Of Dermatology 2019, 181: 192-193. PMID: 30597519, DOI: 10.1111/bjd.17541.Peer-Reviewed Original ResearchAn Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis.
Albano-Aluquin S, Malysz J, Kidacki M, Ratnam M, Olsen NJ. An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis. Journal Of Visualized Experiments 2019 PMID: 30799840, DOI: 10.3791/58713.Peer-Reviewed Original ResearchConceptsGiant cell arteritisFolate receptor betaCell arteritisImmune microenvironmentChronic immune-mediated diseaseImmune-mediated diseasesTemporal artery biopsyT helper lymphocytesLarge-sized arteriesPro-inflammatory responseMyeloid leukemia cellsOcclusive symptomsTotal CD68Disease activityInflammatory disease researchArtery biopsyLymphohistiocytic inflammationSynovial macrophagesSized arteriesCD3 expressionTherapeutic responseVision lossImmunohistopathologic studyPathognomonic featuresIntimal hyperplasiaAn Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
Albano-Aluquin S, Malysz J, Kidacki M, Ratnam M, Olsen N. An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis. Journal Of Visualized Experiments 2019 DOI: 10.3791/58713-v.Peer-Reviewed Case Reports and Technical Notes
2018
Pseudohyperphosphatemia in a patient with incidentally identified progression of smoldering myeloma
Francis ER, Chen F, Kidacki M, Miller R, Alkhasoneh M, Talamo G, Straub M, Klinefelter K, Kodali N, Zhu Y. Pseudohyperphosphatemia in a patient with incidentally identified progression of smoldering myeloma. Clinica Chimica Acta 2018, 487: 306-308. PMID: 30315756, DOI: 10.1016/j.cca.2018.10.016.Peer-Reviewed Original ResearchConceptsSerum phosphorus concentrationHigher serum phosphorus concentrationsKappa/lambda ratioMultiple myelomaLambda chain ratioRare laboratory findingSymptomatic multiple myelomaAppropriate laboratory investigationsLambda ratioLaboratory findingsDisease progressionOrtho VITROSSerial dilutionsCorrect diagnosisPatientsMyelomaPseudohyperphosphatemiaProgressionChain ratioCliniciansLaboratory investigationsGammaglobulinMisdiagnosisVITROSDiagnosisNFkB hyperactivation causes invasion of esophageal squamous cell carcinoma with EGFR overexpression and p120-catenin down-regulation
Lehman HL, Kidacki M, Warrick JI, Stairs DB. NFkB hyperactivation causes invasion of esophageal squamous cell carcinoma with EGFR overexpression and p120-catenin down-regulation. Oncotarget 2018, 9: 11180-11196. PMID: 29541406, PMCID: PMC5834278, DOI: 10.18632/oncotarget.24358.Peer-Reviewed Original ResearchP120-cateninMolecular pathways downstreamInvasive processEGFR overexpressionHuman ESCC cellsCentral regulatorPathways downstreamEsophageal squamous cell carcinomaUpstream regulatorTumor suppressorAggressive cell typesCell typesP120ctnInvasive potentialHuman esophageal keratinocytesEsophageal keratinocytesOverexpressionESCC cellsOrganotypic culture modelRho-kinaseNFkBComplete lossRegulationRegulatorCulture model
2017
p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC
Kidacki M, Lehman HL, Green MV, Warrick JI, Stairs DB. p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC. Molecular Cancer Research 2017, 15: 1398-1409. PMID: 28637905, DOI: 10.1158/1541-7786.mcr-17-0108.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Squamous CellCateninsCell Line, TumorCell MovementClass I Phosphatidylinositol 3-KinasesDelta CateninDown-RegulationGene Expression Regulation, NeoplasticHead and Neck NeoplasmsHumansMatrix Metalloproteinase 1MutationNeoplasm InvasivenessSquamous Cell Carcinoma of Head and NeckConceptsPI3K pathway mutationsNeck squamous cell carcinomaPathway mutationsInhibition of MMP1Squamous cell carcinomaSubset of HNSCCsPotential novel targetAdvanced diseaseCancer Genome AtlasCell carcinomaPIK3CA mutationsHNSCC invasionOral keratinocytesPatient specimensCancer ResHNSCCNovel targetMetastasisMMP1Genome AtlasPotential targetDownregulationIdentification of mechanismsAggressive behaviorInvasionThe Interplay Between p120ctn and EGFR Causes Esophageal Squamous Cell Carcinoma Invasion Through NFkB
Lehman H, Welsh P, Kidacki M, Warrick J, Stairs D. The Interplay Between p120ctn and EGFR Causes Esophageal Squamous Cell Carcinoma Invasion Through NFkB. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.178.11.Peer-Reviewed Original ResearchCell typesInvasion pathwaysGene Ontology functional categoriesInvasive phenotypeTypes of genesGlobal gene analysisUPAR expressionActive RhoA levelsEGFR overexpressionGene expression dataInhibition of RhoEsophageal squamous cell carcinoma invasionFunctional categoriesP120-cateninRhoA GTPaseCentral regulatorEsophageal squamous cell carcinomaActivity downstreamUpstream regulatorTumor suppressorP120ctnGene combinationsCancer genesExpression dataRhoA levels
2016
Tu1175 NFkB Regulates the Invasive Potential of Esophageal Squamous Cell Carcinoma Downstream of EGFR and p120ctn
Lehman H, Welsh P, Kidacki M, Warrick J, Stairs D. Tu1175 NFkB Regulates the Invasive Potential of Esophageal Squamous Cell Carcinoma Downstream of EGFR and p120ctn. Gastroenterology 2016, 150: s854-s855. DOI: 10.1016/s0016-5085(16)32885-2.Peer-Reviewed Original ResearchThe Role of p120‐catenin and PIK3CA in Migration in Head and Neck Squamous Cell Carcinoma
Kidacki M, Lehman H, Welsh P, Warrick J, Stairs D. The Role of p120‐catenin and PIK3CA in Migration in Head and Neck Squamous Cell Carcinoma. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.439.8.Peer-Reviewed Original ResearchNormal oral keratinocytesNeck squamous cell carcinomaSquamous cell carcinomaPIK3CA mutationsCell carcinomaInvasive capabilityCell linesBoyden chamber migration assaysPhospho-STAT3Time of diagnosisCell migrationE545KEpithelial cancer modelsMalignant cell populationMigration/invasionDistant metastasisSame patientCancer aggressivenessOral keratinocytesCancer modelPIK3CA mutantsTumorigenic effectsHNSCCPatientsMetastasis
2012
Higher iron in the red nucleus marks Parkinson's dyskinesia
Lewis MM, Du G, Kidacki M, Patel N, Shaffer ML, Mailman RB, Huang X. Higher iron in the red nucleus marks Parkinson's dyskinesia. Neurobiology Of Aging 2012, 34: 1497-1503. PMID: 23177595, PMCID: PMC3570638, DOI: 10.1016/j.neurobiolaging.2012.10.025.Peer-Reviewed Original ResearchParacrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo
Fleming JM, Miller TC, Kidacki M, Ginsburg E, Stuelten CH, Stewart DA, Troester MA, Vonderhaar BK. Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo. Breast Cancer Research 2012, 14: r97. PMID: 22731827, PMCID: PMC3446360, DOI: 10.1186/bcr3215.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementCell ProliferationChemokinesCytokinesEstrogensFemaleFibroblastsHumansMacrophagesMammary Glands, AnimalMammary Neoplasms, ExperimentalMatrix Metalloproteinase 9MiceMice, Inbred NODMice, SCIDParacrine CommunicationProliferating Cell Nuclear AntigenSignal TransductionStromal CellsTumor Necrosis Factor-alphaConceptsPrimary human macrophagesHuman macrophagesEstrogen stimulationVivo modelParacrine interactionsCell nuclear antigen-positive cellsMammary glandAntigen-positive cellsVivo resultsStromal-derived signalsEpithelial cell proliferationCell interactionsNormal gland developmentTreatment of macrophagesMurine mammary glandMouse mammary glandVivo model systemsWestern analysisBreast functionMMP9 expressionImmunohistochemical analysisPositive cellsImmunohistological analysisMammary microenvironmentTumor growth