Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo
Fleming JM, Miller TC, Kidacki M, Ginsburg E, Stuelten CH, Stewart DA, Troester MA, Vonderhaar BK. Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo. Breast Cancer Research 2012, 14: r97. PMID: 22731827, PMCID: PMC3446360, DOI: 10.1186/bcr3215.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementCell ProliferationChemokinesCytokinesEstrogensFemaleFibroblastsHumansMacrophagesMammary Glands, AnimalMammary Neoplasms, ExperimentalMatrix Metalloproteinase 9MiceMice, Inbred NODMice, SCIDParacrine CommunicationProliferating Cell Nuclear AntigenSignal TransductionStromal CellsTumor Necrosis Factor-alphaConceptsPrimary human macrophagesHuman macrophagesEstrogen stimulationVivo modelParacrine interactionsCell nuclear antigen-positive cellsMammary glandAntigen-positive cellsVivo resultsStromal-derived signalsEpithelial cell proliferationCell interactionsNormal gland developmentTreatment of macrophagesMurine mammary glandMouse mammary glandVivo model systemsWestern analysisBreast functionMMP9 expressionImmunohistochemical analysisPositive cellsImmunohistological analysisMammary microenvironmentTumor growth