Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer
Fox I, Chowdhury N, Gupta S, Kondapalli R, Schilsky M, Stockert R, Chowdhury J. Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin-UDP-glucuronosyltransferase gene transfer. Hepatology 1995, 21: 837-846. DOI: 10.1016/0270-9139(95)90539-1.Peer-Reviewed Original ResearchLarge T antigenSV40 large T antigenT antigenGunn rat hepatocytesImmortalized cellsMutant SV40 large T antigenPrimary hepatocytesRecombinant Moloney murine leukemia virusGene transfer vectorsMoloney murine leukemia virusTransfer vectorsGlutathione S-transferaseMurine leukemia virusCorresponding mRNAExpression of albuminT promoterRat hepatocytesConditional immortalizationASGRGene transferCell growthBilirubin UGTDNA synthesisHepatocyte clonesSynthesized DNAConditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin‐UDP‐glucuronosyltransferase gene transfer
Fox I, Chowdhury N, Gupta S, Kondapalli R, Schilsky M, Stockert R, Chowdhury J. Conditional immortalization of gunn rat hepatocytes: An ex vivo model for evaluating methods for bilirubin‐UDP‐glucuronosyltransferase gene transfer. Hepatology 1995, 21: 837-846. PMID: 7875682, DOI: 10.1002/hep.1840210334.Peer-Reviewed Original ResearchConceptsLarge T antigenSV40 large T antigenGene transfer vectorsT antigenGunn rat hepatocytesMutant SV40 large T antigenImmortalized cellsPrimary hepatocytesTransfer vectorsRecombinant Moloney murine leukemia virusMoloney murine leukemia virusMurine leukemia virusCorresponding mRNAExpression of albuminT promoterRat hepatocytesConditional immortalizationASGRGene transferCell growthViral vectorsBilirubin UGTDNA synthesisUDP-glucuronosyltransferase deficiencyHepatocyte clones