Exon Loss Accounts for Differential Sorting of Na-K-Cl Cotransporters in Polarized Epithelial Cells
Carmosino M, Giménez I, Caplan M, Forbush B. Exon Loss Accounts for Differential Sorting of Na-K-Cl Cotransporters in Polarized Epithelial Cells. Molecular Biology Of The Cell 2008, 19: 4341-4351. PMID: 18667527, PMCID: PMC2555935, DOI: 10.1091/mbc.e08-05-0478.Peer-Reviewed Original ResearchConceptsDileucine motifNa-K-Cl cotransporterRenal Na-K-Cl cotransporterPolarized epithelial cellsAmino acid stretchApical proteinsApical sortingEvolutionary lossRenal epithelial cell lineGene structurePhylogenetic analysisDifferential sortingDirect traffickingEpithelial cell lineAdditional exonC-terminusMammalian kidneyApical membraneExonsNovel mechanismNKCC2 geneCell linesBasolateral membraneMotifEpithelial cellsApical membrane expression of NKCC2 is directed by a domain within its cytoplasmic C‐terminus
Carmosino M, Gimenez I, Caplan M, Forbush B. Apical membrane expression of NKCC2 is directed by a domain within its cytoplasmic C‐terminus. The FASEB Journal 2008, 22: 935.4-935.4. DOI: 10.1096/fasebj.22.1_supplement.935.4.Peer-Reviewed Original ResearchC-terminusNa-K-Cl cotransporterMolecular basisRenal Na-K-Cl cotransporterMDCK cellsCytoplasmic C-terminusTransient expression analysisApical membrane expressionRenal epithelial cell lineBasolateral traffickingResidue stretchEpithelial cell lineApical localizationExpression analysisCentral playerMammalian kidneyApical expressionApical membraneMembrane expressionCorresponding regionLimb cellsCell linesBasolateral membraneThick ascending limb cellsNKCC2