2000
A Transmembrane Segment Determines the Steady-State Localization of an Ion-Transporting Adenosine Triphosphatase
Dunbar L, Aronson P, Caplan M. A Transmembrane Segment Determines the Steady-State Localization of an Ion-Transporting Adenosine Triphosphatase. Journal Of Cell Biology 2000, 148: 769-778. PMID: 10684257, PMCID: PMC2169368, DOI: 10.1083/jcb.148.4.769.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBiological TransportCationsCell LineCell MembraneCell PolarityGlycosphingolipidsGlycosylphosphatidylinositolsH(+)-K(+)-Exchanging ATPaseHydrogen-Ion ConcentrationMembrane ProteinsMolecular Sequence DataOuabainParietal Cells, GastricProtein Sorting SignalsRecombinant Fusion ProteinsSequence AlignmentSequence DeletionSodium-Potassium-Exchanging ATPaseSolubilityTransfectionConceptsK-ATPase alpha subunitAlpha subunitTransmembrane domainPolytopic membrane transport proteinK-ATPaseApical distributionGlycosphingolipid-rich membrane domainsDetergent-insoluble complexesMembrane transport proteinsApical membrane proteinsApical plasma membraneK-ATPase alphaFourth transmembrane domainLocalization signalChimeric pumpsFourth transmembraneTransmembrane segmentsK-ATPase sequencesMembrane compartmentsMembrane domainsMembrane proteinsSequence domainsPlasma membraneGastric parietal cellsTransport proteins
1993
Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly
Gottardi CJ, Caplan MJ. Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly. Journal Of Biological Chemistry 1993, 268: 14342-14347. PMID: 8390991, DOI: 10.1016/s0021-9258(19)85246-8.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunitK-ATPase beta subunitAlpha subunitBeta subunitCell surface expressionTerminal halfK-ATPaseCell surface deliveryEfficient cell surface expressionK-ATPase alphaNH2-terminal halfCOS-1 cellsIon-transporting ATPasesProtein domainsK-ATPase enzymeSubunit assemblySurface deliveryIntracellular vesiclesSubunit chimerasIndividual subunitsActive enzymeMolecular requirementsSubunitsCell surfaceBeta protein
1992
Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.
Pietrini G, Matteoli M, Banker G, Caplan MJ. Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 8414-8418. PMID: 1326755, PMCID: PMC49930, DOI: 10.1073/pnas.89.18.8414.Peer-Reviewed Original ResearchConceptsHippocampal neuronsAlpha 1Epithelial cellsMature cultured hippocampal neuronsCultured hippocampal neuronsK-ATPase alpha subunitPolarized epithelial cell lineAlpha 3 proteinAlpha 3 isoformDistribution of isoformsEpithelial cell lineRenal epithelial cellsInfluenza glycoproteinsVesicular stomatitis virusNeuronal cellsNeuronsAlpha subunitCell linesStable transfectionStomatitis virusAxonsK-ATPaseIsoformsCellsDendrites
1986
Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells.
Caplan MJ, Palade GE, Jamieson JD. Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells. Journal Of Biological Chemistry 1986, 261: 2860-2865. PMID: 3005269, DOI: 10.1016/s0021-9258(17)35866-0.Peer-Reviewed Original ResearchConceptsAlpha subunitAnimal cell plasma membranesK-ATPase alpha subunitMDCK cellsMembrane-bound vesiclesCell plasma membraneCytosol fractionTransmembrane proteinCrude cytosolic fractionPlasma membraneMembranous vesiclesPrecursor-product relationshipSoluble precursorsMembrane vesiclesK-ATPaseCytosolic fractionMembrane pelletVesiclesStaphylococcus aureus cellsAureus cellsCellsMonoclonal antibodiesProteinPrecursorsMembrane
1985
Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.
Schneider JW, Mercer RW, Caplan M, Emanuel JR, Sweadner KJ, Benz EJ, Levenson R. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 6357-6361. PMID: 2994074, PMCID: PMC391053, DOI: 10.1073/pnas.82.18.6357.Peer-Reviewed Original ResearchConceptsRat brain NaBrain NaK-ATPase alpha subunitRat brainAlpha subunitCDNA clonesK-ATPase antibodiesCell linesFusion proteinCDNA insertK-ATPaseLambda gt11 cDNA expression libraryAlpha-subunit cDNACDNA fusion proteinDifferent rat tissuesK-ATPase polypeptidesTissue-specific patternsPositive phage clonesAmino acid sequenceAntibody reactiveCDNA expression libraryParental HeLa cellsMonoclonal antibodiesDog kidneyHuman cell lines