2009
POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels*
Lin DH, Yue P, Pan CY, Sun P, Zhang X, Han Z, Roos M, Caplan M, Giebisch G, Wang WH. POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels*. Journal Of Biological Chemistry 2009, 284: 29614-29624. PMID: 19710010, PMCID: PMC2785594, DOI: 10.1074/jbc.m109.041582.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBiological TransportCell LineClathrinDynaminsEpithelial Sodium ChannelsGene Expression RegulationHumansKidney Tubules, CollectingOocytesPotassium Channels, Inwardly RectifyingProtein Sorting SignalsProtein Structure, TertiaryRatsRats, Sprague-DawleyUbiquitinationUbiquitin-Protein LigasesXenopus laevisConceptsHEK293T cellsClathrin-independent endocytosisE3 ubiquitin ligaseUbiquitin ligaseGlutathione S-transferase pulldown experimentsROMK1 channelsT cellsTyrosine-based internalization signalPotassium currentROMK channelsDominant-negative dynaminImmunoprecipitation of lysatesInternalization signalInhibitory effectPulldown experimentsScaffold proteinUbiquitination assaysRING domainUbiquitinationN-terminusGamma subunitsAmino acidsENaC-alphaROMK1Tissue lysates
2008
POSH decreases ROMK1 channel activity through stimulating clatharin‐independent and dynamin‐dependent endocytosis.
Lin D, Yue P, Sun P, Jin Y, Roos M, Caplan M, Wang W. POSH decreases ROMK1 channel activity through stimulating clatharin‐independent and dynamin‐dependent endocytosis. The FASEB Journal 2008, 22: 1180.1-1180.1. DOI: 10.1096/fasebj.22.1_supplement.1180.1.Peer-Reviewed Original ResearchExpression of Tetraspan Protein CD63 Activates Protein-tyrosine Kinase (PTK) and Enhances the PTK-induced Inhibition of ROMK Channels*
Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH. Expression of Tetraspan Protein CD63 Activates Protein-tyrosine Kinase (PTK) and Enhances the PTK-induced Inhibition of ROMK Channels*. Journal Of Biological Chemistry 2008, 283: 7674-7681. PMID: 18211905, DOI: 10.1074/jbc.m705574200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBenzoquinonesCSK Tyrosine-Protein KinaseEnzyme InhibitorsFemaleGene Expression RegulationHumansKidney CortexKidney MedullaLactams, MacrocyclicMaleOocytesOrgan SpecificityPatch-Clamp TechniquesPhosphorylationPlatelet Membrane GlycoproteinsPotassium Channels, Inwardly RectifyingProtein-Tyrosine KinasesProto-Oncogene ProteinsRatsRats, Sprague-DawleyReceptor-Like Protein Tyrosine Phosphatases, Class 4RifabutinSrc-Family KinasesTetraspanin 30TransfectionXenopus laevisConceptsExpression of CD63T cellsOuter medullaRenal cortexROMK channelsProtein tyrosine kinasesC-SrcRole of CD63Potassium restrictionROMK activityPotassium currentTwo-electrode voltage clampRat kidneyDecreased expressionImmunocytochemical stainingROMK1 channelsInhibitory effectMedullaNative rat kidneyCD63Voltage clampCortexRPTPalphaTyrosine phosphorylationHerbimycin A
2007
Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels
Lin D, Kamsteeg R, Sterling H, Jin Y, Zhang Y, Roos M, Spencer J, Caplan M, Wang W. Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels. The FASEB Journal 2007, 21: a1331-a1332. DOI: 10.1096/fasebj.21.6.a1331-d.Peer-Reviewed Original ResearchInhibition of PTKROMK channelsInhibitory effectK currentsHEK293 cellsC-SrcRenal outer medullaProtein tyrosine kinasesRole of CD63Tyrosine phosphorylationOuter medullaDecreased expressionTetraspanin protein CD63CD63Voltage clampElectrode voltage clampInhibitionHerbimycin AOocytesTyrosine kinaseXenopus oocytesPresent studyActivated PKCTetraspanin proteinsCoexpression