2011
AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦
Zhang L, Jouret F, Rinehart J, Sfakianos J, Mellman I, Lifton RP, Young LH, Caplan MJ. AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦. Journal Of Biological Chemistry 2011, 286: 16879-16890. PMID: 21383016, PMCID: PMC3089531, DOI: 10.1074/jbc.m110.186932.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsCadherinsCalciumCell AdhesionCell MembraneDogsEpitheliumGene Expression Regulation, EnzymologicGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaMembrane ProteinsMicroscopy, FluorescencePhosphoproteinsPhosphorylationRNA InterferenceTight JunctionsZonula Occludens-1 ProteinConceptsProtein kinase activationTight junction componentsJunction componentsPlasma membraneAMPK activationKinase activationGSK-3β inhibitionNectin-afadin systemEpithelial tight junctionsTight junctionsPhosphorylation studiesSynthase kinaseJunctional proteinsAbsence of extracellularDistinct pathwaysCell growthE-cadherinIndependent depositionKinaseActivationInduce Ca2MembraneAfadinExtracellularInhibition
2007
Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels
Lin D, Kamsteeg R, Sterling H, Jin Y, Zhang Y, Roos M, Spencer J, Caplan M, Wang W. Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels. The FASEB Journal 2007, 21: a1331-a1332. DOI: 10.1096/fasebj.21.6.a1331-d.Peer-Reviewed Original ResearchInhibition of PTKROMK channelsInhibitory effectK currentsHEK293 cellsC-SrcRenal outer medullaProtein tyrosine kinasesRole of CD63Tyrosine phosphorylationOuter medullaDecreased expressionTetraspanin protein CD63CD63Voltage clampElectrode voltage clampInhibitionHerbimycin AOocytesTyrosine kinaseXenopus oocytesPresent studyActivated PKCTetraspanin proteinsCoexpression