2015
Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin
Farr GA, Hull M, Stoops EH, Bateson R, Caplan MJ. Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin. Molecular Biology Of The Cell 2015, 26: 4401-4411. PMID: 26424804, PMCID: PMC4666135, DOI: 10.1091/mbc.e14-09-1385.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkPlasma membraneE-cadherinK-ATPasePolarized MDCK epithelial cellsPost-Golgi traffickingCell surfacePolarized epithelial cellsEpithelial cellsMDCK epithelial cellsDistinct trafficking routesBiosynthetic traffickingCarrier vesiclesSecretory pathwayMembrane proteinsSurface deliveryBasolateral domainMost proteinsTrafficking routesGolgi complexTemperature blockTraffickingProteinMembraneCells
2011
AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦
Zhang L, Jouret F, Rinehart J, Sfakianos J, Mellman I, Lifton RP, Young LH, Caplan MJ. AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦. Journal Of Biological Chemistry 2011, 286: 16879-16890. PMID: 21383016, PMCID: PMC3089531, DOI: 10.1074/jbc.m110.186932.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsCadherinsCalciumCell AdhesionCell MembraneDogsEpitheliumGene Expression Regulation, EnzymologicGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaMembrane ProteinsMicroscopy, FluorescencePhosphoproteinsPhosphorylationRNA InterferenceTight JunctionsZonula Occludens-1 ProteinConceptsProtein kinase activationTight junction componentsJunction componentsPlasma membraneAMPK activationKinase activationGSK-3β inhibitionNectin-afadin systemEpithelial tight junctionsTight junctionsPhosphorylation studiesSynthase kinaseJunctional proteinsAbsence of extracellularDistinct pathwaysCell growthE-cadherinIndependent depositionKinaseActivationInduce Ca2MembraneAfadinExtracellularInhibition
2010
Exosome release of β-catenin: a novel mechanism that antagonizes Wnt signaling
Chairoungdua A, Smith DL, Pochard P, Hull M, Caplan MJ. Exosome release of β-catenin: a novel mechanism that antagonizes Wnt signaling. Journal Of Cell Biology 2010, 190: 1079-1091. PMID: 20837771, PMCID: PMC3101591, DOI: 10.1083/jcb.201002049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBeta CateninCadherinsCell LineExosomesGene Knockdown TechniquesGenes, ReporterGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHumansKangai-1 ProteinLysosomesMembrane GlycoproteinsMiceModels, BiologicalProteasome Endopeptidase ComplexProtein Processing, Post-TranslationalProtein StabilityProtein TransportSignal TransductionTetraspanin 29TransfectionWnt ProteinsConceptsΒ-cateninΒ-catenin-mediated WntProtein degradation pathwaysCellular signaling pathwaysTetraspanin membrane proteinΒ-catenin protein levelsGlycogen synthase kinase-3βWnt/β-catenin signalingSynthase kinase-3βΒ-catenin signalingMembrane proteinsCytosolic proteinsSignaling pathwaysKinase-3βExosomal packagingExosome releaseSphingomyelinase inhibitorNovel mechanismE-cadherinDegradation pathwayProtein levelsCD82Tumor metastasisCD9WntExosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling
Chairoungdua A, Smith D, Pochard P, Hull M, Caplan M. Exosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling. The FASEB Journal 2010, 24: 715.3-715.3. DOI: 10.1096/fasebj.24.1_supplement.715.3.Peer-Reviewed Original ResearchWnt/β-cateninWnt/β-catenin activityDendritic cellsΒ-cateninΒ-catenin activityCD9 expressionE-cadherinTumor metastasisWild-type miceNovel mechanismΒ-catenin protein levelsT cellsΒ-catenin levelsKnockout miceTumor cell metastasisCell metastasisGSK-3βMetastasisCHO cellsLuciferase reporterInappropriate activationCD82 expressionProtein levelsHEK 293T cellsSignificant decrease
1997
Epithelial Cell Polarity: Challenges and Methodologies
Caplan M, Rodriguez‐Boulan E. Epithelial Cell Polarity: Challenges and Methodologies. 1997, 665-688. DOI: 10.1002/cphy.cp140117.Peer-Reviewed Original ResearchEpithelial polarityProtein trafficking pathwaysEpithelial cell polarityTissue-specific variationsEpithelial cellsSorting machineryProtein polarityCell polaritySorting signalsTargeting pathwaysTrafficking pathwaysRecycling pathwayBiochemical techniquesE-cadherinVitro systemEpithelial monolayersGenetic modelsPathwayBiogenetic pathwayMorphological techniquesCellsPolarityMachinerySubstrataTranscytosis