2010
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro
Pochard P, Vickery B, Berin MC, Grishin A, Sampson HA, Caplan M, Bottomly K. Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro. Journal Of Allergy And Clinical Immunology 2010, 126: 92-97.e5. PMID: 20538332, PMCID: PMC2902661, DOI: 10.1016/j.jaci.2010.04.003.Peer-Reviewed Original ResearchConceptsToll-like receptorsCrude peanut extractDendritic cellsT cellsBacterial adjuvantDC maturationCytokine productionBone marrow-derived dendritic cellsMarrow-derived dendritic cellsDelivery of allergensFood allergy treatmentOngoing allergic responseSuccessful immunotherapeutic strategiesIFN-gamma levelsT cell responsesPrimed T cellsT cell proliferationInnate immune systemCarboxyfluorescein succinimidyl esterAllergen immunotherapySensitized miceBioPlex assayIL-17Immunotherapeutic strategiesTh2 responsesExosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling
Chairoungdua A, Smith D, Pochard P, Hull M, Caplan M. Exosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling. The FASEB Journal 2010, 24: 715.3-715.3. DOI: 10.1096/fasebj.24.1_supplement.715.3.Peer-Reviewed Original ResearchWnt/β-cateninWnt/β-catenin activityDendritic cellsΒ-cateninΒ-catenin activityCD9 expressionE-cadherinTumor metastasisWild-type miceNovel mechanismΒ-catenin protein levelsT cellsΒ-catenin levelsKnockout miceTumor cell metastasisCell metastasisGSK-3βMetastasisCHO cellsLuciferase reporterInappropriate activationCD82 expressionProtein levelsHEK 293T cellsSignificant decrease
2009
Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy
Demento SL, Eisenbarth SC, Foellmer HG, Platt C, Caplan MJ, Saltzman W, Mellman I, Ledizet M, Fikrig E, Flavell RA, Fahmy TM. Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy. Vaccine 2009, 27: 3013-3021. PMID: 19428913, PMCID: PMC2695996, DOI: 10.1016/j.vaccine.2009.03.034.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAnimalsAntibody FormationCarrier ProteinsCD8-Positive T-LymphocytesDendritic CellsLactic AcidLipopolysaccharidesMiceMice, Inbred C57BLNanoparticlesNLR Family, Pyrin Domain-Containing 3 ProteinPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerVaccinationViral Envelope ProteinsWest Nile FeverWest Nile Virus VaccinesConceptsPattern recognition receptorsToll-like receptorsInflammasome activationInnate immune system activationEffective adaptive immune responseIntracellular pattern recognition receptorsAntigen-presenting cellsAdaptive immune responsesWest Nile encephalitisImmune system activationInnate immune pathwaysWild-type macrophagesDendritic cellsCellular immunityVaccination approachesVaccine efficacyIL-1betaNLRP3 inflammasomeAdjuvant systemImmune responsePotent new approachMurine modelInflammasome activitySystem activationImmune pathways