2013
Activation of the Ca2+-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane
Jouret F, Wu J, Hull M, Rajendran V, Mayr B, Schöfl C, Geibel J, Caplan MJ. Activation of the Ca2+-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane. Journal Of Cell Science 2013, 126: 5132-5142. PMID: 24013548, PMCID: PMC3828589, DOI: 10.1242/jcs.127555.Peer-Reviewed Original ResearchConceptsTJ assemblyMDCK cellsStable transfectionFunction mutant formZO-1Tight junction components ZO-1G protein-coupled receptorsHuman CaSRCell-cell contactEpithelial cell plasma membranesMadin-Darby canine kidney cellsCell plasma membraneStimulation of CaSRDivalent ion homeostasisCanine kidney cellsTight junctionsJunction-associated proteinsTight junction componentsEndogenous CaSRProtein kinasePlasma membraneIon homeostasisMutant formsChelator BAPTA-AMCell differentiation
1986
Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells.
Caplan MJ, Palade GE, Jamieson JD. Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells. Journal Of Biological Chemistry 1986, 261: 2860-2865. PMID: 3005269, DOI: 10.1016/s0021-9258(17)35866-0.Peer-Reviewed Original ResearchConceptsAlpha subunitAnimal cell plasma membranesK-ATPase alpha subunitMDCK cellsMembrane-bound vesiclesCell plasma membraneCytosol fractionTransmembrane proteinCrude cytosolic fractionPlasma membraneMembranous vesiclesPrecursor-product relationshipSoluble precursorsMembrane vesiclesK-ATPaseCytosolic fractionMembrane pelletVesiclesStaphylococcus aureus cellsAureus cellsCellsMonoclonal antibodiesProteinPrecursorsMembrane