2008
Apical membrane expression of NKCC2 is directed by a domain within its cytoplasmic C‐terminus
Carmosino M, Gimenez I, Caplan M, Forbush B. Apical membrane expression of NKCC2 is directed by a domain within its cytoplasmic C‐terminus. The FASEB Journal 2008, 22: 935.4-935.4. DOI: 10.1096/fasebj.22.1_supplement.935.4.Peer-Reviewed Original ResearchC-terminusNa-K-Cl cotransporterMolecular basisRenal Na-K-Cl cotransporterMDCK cellsCytoplasmic C-terminusTransient expression analysisApical membrane expressionRenal epithelial cell lineBasolateral traffickingResidue stretchEpithelial cell lineApical localizationExpression analysisCentral playerMammalian kidneyApical expressionApical membraneMembrane expressionCorresponding regionLimb cellsCell linesBasolateral membraneThick ascending limb cellsNKCC2
2002
Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membrane
Deen PM, Van Balkom BW, Savelkoul PJ, Kamsteeg EJ, Van Raak M, Jennings ML, Muth TR, Rajendran V, Caplan MJ. Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membrane. American Journal Of Physiology. Renal Physiology 2002, 282: f330-f340. PMID: 11788448, DOI: 10.1152/ajprenal.0168.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAquaporin 1Aquaporin 2Aquaporin 6AquaporinsArginine VasopressinBlood Group AntigensCell CompartmentationCell FractionationCell LineCell MembraneCell Membrane PermeabilityColforsinEndocytosisGene ExpressionHumansKidneyProtein Structure, TertiaryProtein TransportRatsRecombinant Fusion ProteinsVasoconstrictor AgentsWaterConceptsIntracellular vesiclesApical membraneAquaporin-2Wild‐type aquaporin‐2Mammalian water homeostasisMadin-Darby canine kidney cellsCanine kidney cellsAQP2 accumulationPrimary sequenceSame proteinOsmotic water permeabilityApical expressionForskolin treatmentAquaporin-1 (AQP1) water channelWater homeostasisKidney cellsBasolateral membraneVesiclesPlacental alkaline phosphataseMembraneWater channelsDuct cellsAQP1TailCells