2006
An Extracellular Loop of the Human Non-Gastric H,K-ATPase a-subunit is Involved in Apical Plasma Membrane Polarization
Lerner M, Lemke D, Bertram H, Schillers H, Oberleithner H, Caplan MJ, Reinhardt J. An Extracellular Loop of the Human Non-Gastric H,K-ATPase a-subunit is Involved in Apical Plasma Membrane Polarization. Cellular Physiology And Biochemistry 2006, 18: 75-84. PMID: 16914892, DOI: 10.1159/000095169.Peer-Reviewed Original ResearchConceptsP-type ATPasesSorting motifApical deliveryExtracellular loopK-ATPaseSpecific sorting signalsPlasma membrane polarizationShort extracellular loopApical plasma membraneMadin-Darby canine kidney cellsSingle point mutationCanine kidney cellsSorting signalsGene familyPlasma membraneFlanking regionsEpithelial apical membraneK-ATPasesPhysiological roleApical membraneCellular distributionPoint mutationsIon pumpsATP1AL1Corresponding region
2000
Differential localization of human nongastric H+-K+-ATPase ATP1AL1 in polarized renal epithelial cells
Reinhardt J, Grishin A, Oberleithner H, Caplan M. Differential localization of human nongastric H+-K+-ATPase ATP1AL1 in polarized renal epithelial cells. American Journal Of Physiology. Renal Physiology 2000, 279: f417-f425. PMID: 10966921, DOI: 10.1152/ajprenal.2000.279.3.f417.Peer-Reviewed Original ResearchConceptsApical plasma membranePlasma membraneRenal epithelial cellsIon pumpsPlasma membrane localizationConfocal immunofluorescence microscopyEpithelial cellsATPase beta subunitRenal epithelial cell lineMembrane localizationLow expression levelsEpithelial cell lineSurface biotinylationPump subunitsBeta subunitFunctional expressionStable transfectionLateral membranesMDCK cellsATP1AL1Immunofluorescence microscopyDifferential localizationSorting mechanismStable interactionExpression levels
1998
ATP1AL1, a Member of the Non-gastric H,K-ATPase Family, Functions as a Sodium Pump*
Grishin A, Caplan M. ATP1AL1, a Member of the Non-gastric H,K-ATPase Family, Functions as a Sodium Pump*. Journal Of Biological Chemistry 1998, 273: 27772-27778. PMID: 9774385, DOI: 10.1074/jbc.273.43.27772.Peer-Reviewed Original Research
1996
Functional expression of the cDNA encoded by the human ATP1AL1 gene
Grishin AV, Bevensee MO, Modyanov NN, Rajendran V, Boron WF, Caplan MJ. Functional expression of the cDNA encoded by the human ATP1AL1 gene. American Journal Of Physiology 1996, 271: f539-f551. PMID: 8853415, DOI: 10.1152/ajprenal.1996.271.3.f539.Peer-Reviewed Original ResearchConceptsHuman ATP1AL1 geneAcute loweringNH4Cl pulseUptake activityHEK-293 cellsSpontaneous intracellularMM ouabainProtein expressionCOS cellsATP1AL1Polyclonal antibodiesPH-sensitive dyeOuabainBeta-subunit cDNAExtrusion activityCellsK-ATPaseTransfection studiesSame cellsFunctional expressionBeta complexExpressionDependent ATPaseEndplasmic reticulumProtein