2011
Interactions between β-Catenin and the HSlo Potassium Channel Regulates HSlo Surface Expression
Bian S, Bai JP, Chapin H, Le Moellic C, Dong H, Caplan M, Sigworth FJ, Navaratnam DS. Interactions between β-Catenin and the HSlo Potassium Channel Regulates HSlo Surface Expression. PLOS ONE 2011, 6: e28264. PMID: 22194818, PMCID: PMC3237428, DOI: 10.1371/journal.pone.0028264.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBeta CateninBinding SitesBiological AssayCell MembraneChickensGene Knockdown TechniquesHair Cells, AuditoryHEK293 CellsHumansImmunoprecipitationIntercellular JunctionsKineticsLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsModels, MolecularMolecular Sequence DataMutant ProteinsMutationPhosphorylationProtein BindingProtein TransportRNA, Small InterferingSequence DeletionTransfectionWnt Signaling PathwayConceptsΒ-cateninS10 regionHEK cellsSurface expressionCell biology toolsPotassium channel alpha subunitΒ-catenin interactionDownregulation of WntCytoskeleton frameworkChannel alpha subunitChicken hair cellsPhosphorylation sitesDeletion mutantsBiology toolsΒ-catenin-dependent canonical WntAlpha subunitCanonical WntMultiple binding sitesNumber of diseasesStable bindingWntPhysiological significanceBinding sitesReduced expressionHair cells
2010
Exosome release of β-catenin: a novel mechanism that antagonizes Wnt signaling
Chairoungdua A, Smith DL, Pochard P, Hull M, Caplan MJ. Exosome release of β-catenin: a novel mechanism that antagonizes Wnt signaling. Journal Of Cell Biology 2010, 190: 1079-1091. PMID: 20837771, PMCID: PMC3101591, DOI: 10.1083/jcb.201002049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBeta CateninCadherinsCell LineExosomesGene Knockdown TechniquesGenes, ReporterGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHumansKangai-1 ProteinLysosomesMembrane GlycoproteinsMiceModels, BiologicalProteasome Endopeptidase ComplexProtein Processing, Post-TranslationalProtein StabilityProtein TransportSignal TransductionTetraspanin 29TransfectionWnt ProteinsConceptsΒ-cateninΒ-catenin-mediated WntProtein degradation pathwaysCellular signaling pathwaysTetraspanin membrane proteinΒ-catenin protein levelsGlycogen synthase kinase-3βWnt/β-catenin signalingSynthase kinase-3βΒ-catenin signalingMembrane proteinsCytosolic proteinsSignaling pathwaysKinase-3βExosomal packagingExosome releaseSphingomyelinase inhibitorNovel mechanismE-cadherinDegradation pathwayProtein levelsCD82Tumor metastasisCD9WntExosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling
Chairoungdua A, Smith D, Pochard P, Hull M, Caplan M. Exosome‐release of beta‐catenin: A novel mechanism to antagonize Wnt signaling. The FASEB Journal 2010, 24: 715.3-715.3. DOI: 10.1096/fasebj.24.1_supplement.715.3.Peer-Reviewed Original ResearchWnt/β-cateninWnt/β-catenin activityDendritic cellsΒ-cateninΒ-catenin activityCD9 expressionE-cadherinTumor metastasisWild-type miceNovel mechanismΒ-catenin protein levelsT cellsΒ-catenin levelsKnockout miceTumor cell metastasisCell metastasisGSK-3βMetastasisCHO cellsLuciferase reporterInappropriate activationCD82 expressionProtein levelsHEK 293T cellsSignificant decrease
2008
Polycystin-1 C-terminal tail associates with β-catenin and inhibits canonical Wnt signaling
Lal M, Song X, Pluznick JL, Di Giovanni V, Merrick DM, Rosenblum ND, Chauvet V, Gottardi CJ, Pei Y, Caplan MJ. Polycystin-1 C-terminal tail associates with β-catenin and inhibits canonical Wnt signaling. Human Molecular Genetics 2008, 17: 3105-3117. PMID: 18632682, PMCID: PMC2722884, DOI: 10.1093/hmg/ddn208.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBinding SitesCell LineCell NucleusCHO CellsCricetinaeCricetulusGene Expression ProfilingGene Expression RegulationHumansLigandsOligonucleotide Array Sequence AnalysisPeptide FragmentsPolycystic Kidney, Autosomal DominantRecombinant ProteinsSignal TransductionSystems BiologyTCF Transcription FactorsTransfectionTRPP Cation ChannelsWnt ProteinsConceptsC-terminal tailCanonical WntPolycystin-1Wnt-dependent signalingDNA microarray analysisDependent gene transcriptionN-terminal portionInhibits canonical WntTCF proteinsT-cell factorAutosomal dominant polycystic kidney disease (ADPKD) casesMotif presentGene transcriptionDevelopmental processesMicroarray analysisWntPKD1 geneΒ-cateninNovel mechanism