2019
Early Porcine Sapovirus Infection Disrupts Tight Junctions and Uses Occludin as a Coreceptor
Alfajaro M, Cho E, Kim D, Kim J, Park J, Soliman M, Baek Y, Park C, Kang M, Park S, Cho K. Early Porcine Sapovirus Infection Disrupts Tight Junctions and Uses Occludin as a Coreceptor. Journal Of Virology 2019, 93: 10.1128/jvi.01773-18. PMID: 30463963, PMCID: PMC6364031, DOI: 10.1128/jvi.01773-18.Peer-Reviewed Original ResearchConceptsSevere acute gastroenteritisClaudin-1Acute gastroenteritisEntry factorsTight junctionsTJ proteinsLLC-PK cellsAdhesion molecule-1Common causative agentChinese hamster ovaryDisrupts tight junctionsIntestinal epithelial cellsTransepithelial electrical resistanceHisto-blood groupTJ protein occludinRole of TJsMolecule-1Functional coreceptorInfectionTerminal sialic acidAffordable drugsProtein occludinOccludinSpecific antibodiesEpithelial cells
2018
Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II
Soliman M, Kim D, Kim C, Seo J, Kim J, Park J, Alfajaro M, Baek Y, Cho E, Park S, Kang M, Chang K, Goodfellow I, Cho K. Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II. Veterinary Research 2018, 49: 92. PMID: 30223898, PMCID: PMC6142377, DOI: 10.1186/s13567-018-0584-0.Peer-Reviewed Original ResearchConceptsDynamin IIActin rearrangementLate endosomesDN mutantsLLC-PK cellsDynamin GTPase activityClathrin-mediated endocytosisCowden strainClathrin-mediated internalizationDominant negative mutantCholesterol-dependent endocytosisInhibition of caveolaeVesicle internalizationCholesterol-sequestering drugEndosomal traffickingEarly endosomesSiRNA depletionNegative mutantGTPase activityClathrinEndocytosisEndosomal acidificationMutantsEndosomesCell entryBovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens
Cho E, Soliman M, Alfajaro M, Kim J, Seo J, Park J, Kim D, Baek Y, Kang M, Park S, Le Pendu J, Cho K. Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens. Journal Of Virology 2018, 92: 10.1128/jvi.02160-17. PMID: 29467317, PMCID: PMC5899197, DOI: 10.1128/jvi.02160-17.Peer-Reviewed Original ResearchConceptsFucose epitopesTerminal sialic acidHost speciesVirus-like particlesHisto-blood group antigensCell surface carbohydrate moietiesMammalian host speciesEnteric calicivirusesAttachment factorsSialic acidCultured cell linesBovine digestive tractSurface carbohydrate moietiesCell carbohydratesHost rangeType speciesViral generaHBGA recognitionCarbohydrate moietyBiochemical assaysSpecific enzymesCellular receptorsSynthetic histo-blood group antigensReplication cycleSpecies
2014
Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus
Kim D, Hosmillo M, Alfajaro M, Kim J, Park J, Son K, Ryu E, Sorgeloos F, Kwon H, Park S, Lee W, Cho D, Kwon J, Choi J, Kang M, Goodfellow I, Cho K. Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus. PLOS Pathogens 2014, 10: e1004172. PMID: 24901849, PMCID: PMC4047124, DOI: 10.1371/journal.ppat.1004172.Peer-Reviewed Original ResearchConceptsHisto-blood group antigensFunctional receptorsSambucus nigra lectinSialic acidGroup antigensTreatment of cellsSynthetic histo-blood group antigensViral attachmentVirus bindingMaackia amurensis lectinPorcine sapovirusVibrio cholerae neuraminidaseRed blood cellsIntestinal tissue sectionsAcute gastroenteritisImportant causeInfectionBlood cellsVirus attachmentReceptorsPSAVSapovirusCellular receptorsTissue sectionsGlycoprotein acts