2019
Dual Recognition of Sialic Acid and αGal Epitopes by the VP8* Domains of the Bovine Rotavirus G6P[5] WC3 and of Its Mono-reassortant G4P[5] RotaTeq Vaccine Strains
Alfajaro M, Kim J, Barbé L, Cho E, Park J, Soliman M, Baek Y, Kang M, Kim S, Kim G, Park S, Le Pendu J, Cho K. Dual Recognition of Sialic Acid and αGal Epitopes by the VP8* Domains of the Bovine Rotavirus G6P[5] WC3 and of Its Mono-reassortant G4P[5] RotaTeq Vaccine Strains. Journal Of Virology 2019, 93: 10.1128/jvi.00941-19. PMID: 31243129, PMCID: PMC6714814, DOI: 10.1128/jvi.00941-19.Peer-Reviewed Original ResearchConceptsHisto-blood group antigensRotaTeq vaccineIntestinal epithelial cellsAlternative receptorSialic acidVaccine strainGroup antigensSevere rotavirus diseaseEpithelial cellsHuman intestinal epithelial cellsHuman small intestinal epithelial cellsNatural human infectionBearing strainsSmall intestinal epithelial cellsRotaTeq vaccine strainsMA-104 cellsTight junction proteinsGroup A rotavirusesRotavirus diseaseSevere diarrheaIntestinal enteroidsGroup ASurface sialic acidΑGal epitopesImportant causeDevelopment of a live attenuated trivalent porcine rotavirus A vaccine against disease caused by recent strains most prevalent in South Korea
Park J, Alfajaro M, Cho E, Kim J, Soliman M, Baek Y, Park C, Lee J, Son K, Cho K, Kang M. Development of a live attenuated trivalent porcine rotavirus A vaccine against disease caused by recent strains most prevalent in South Korea. Veterinary Research 2019, 50: 2. PMID: 30616694, PMCID: PMC6323864, DOI: 10.1186/s13567-018-0619-6.Peer-Reviewed Original ResearchConceptsVaccine strainTrivalent vaccineKorean swine industrySevere economic lossesHomologous virulent strainPorcine rotavirusSwine industryEconomic lossesSerum virus-neutralizing antibodiesExperimental pigletsVirulent strainCell culture passageFecal secretory IgAChallenge exposureVirus-neutralizing antibodiesRecent strainsPigletsVirulentConsecutive passagesRotavirus diseaseIgA levelsRotavirus vaccineOral immunizationHistopathological lesionsVaccine
2018
Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells
Soliman M, Cho E, Park J, Kim J, Alfajaro M, Baek Y, Kim D, Kang M, Park S, Cho K. Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells. Scientific Reports 2018, 8: 13931. PMID: 30224682, PMCID: PMC6141481, DOI: 10.1038/s41598-018-32352-y.Peer-Reviewed Original ResearchConceptsTJ protein distributionTJ integrityTJ proteinsTight junctionsIntestinal epithelial tight junctionsEarly disruptionMDCK cellsEpithelial tight junctionsPrecise molecular mechanismsBovine NCDVRotavirus strainsEarly activationParacellular permeabilityCellular receptorsPerijunctional actomyosin ringTransepithelial resistanceEntry portalReversible decreaseSignaling pathwaysInfectionCoreceptorHarmful factorsMolecular mechanismsPresent studyCellsActivation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating
Soliman M, Seo J, Kim D, Kim J, Park J, Alfajaro M, Baek Y, Cho E, Kwon J, Choi J, Kang M, Park S, Cho K. Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating. PLOS Pathogens 2018, 14: e1006820. PMID: 29352319, PMCID: PMC5792019, DOI: 10.1371/journal.ppat.1006820.Peer-Reviewed Original ResearchMeSH KeywordsAcidsAnimalsCaco-2 CellsCapsid ProteinsCattleCells, CulturedEndosomesEnzyme ActivationExtracellular Signal-Regulated MAP KinasesHaplorhiniHumansHydrogen-Ion ConcentrationPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRotavirusRotavirus InfectionsSf9 CellsSignal TransductionVacuolar Proton-Translocating ATPasesVirus UncoatingConceptsMEK/ERK pathwayV-ATPasePI3K/AktSubunit EOuter capsid proteinEndosomal acidificationLate endosomesERK pathwayCapsid proteinPI3KEarly infection eventsMEK/ERKCell surface receptorsImmunoprecipitation assaysPPI3KVirus traffickingProximity ligationMultistep bindingSurface receptorsAktViral progenyViral uncoatingERKRVA strainsEndosomes
2017
Glycan-specificity of four neuraminidase-sensitive animal rotavirus strains
Kim J, Kim D, Seo J, Park J, Alfajaro M, Soliman M, Baek Y, Cho E, Kwon H, Park S, Kang M, Cho K. Glycan-specificity of four neuraminidase-sensitive animal rotavirus strains. Veterinary Microbiology 2017, 207: 159-163. PMID: 28757018, DOI: 10.1016/j.vetmic.2017.06.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineFibroblastsHumansMembrane GlycoproteinsNeuraminidaseRotavirusVirus Attachment
2016
Whole genomic characterization of Korean porcine G8P[7] reassortant rotaviruses
Park J, Park S, Woo N, Kim D, Seo J, Alfajaro M, Kim J, Soliman M, Baek Y, Cho E, Kwon J, Choi J, Kang M, Matthijnssens J, Cho K. Whole genomic characterization of Korean porcine G8P[7] reassortant rotaviruses. Archives Of Virology 2016, 161: 2835-2841. PMID: 27393603, DOI: 10.1007/s00705-016-2945-1.Peer-Reviewed Original Research
2015
Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses
Jeong Y, Matthijnssens J, Kim D, Kim J, Alfajaro M, Park J, Hosmillo M, Son K, Soliman M, Baek Y, Kwon J, Choi J, Kang M, Cho K. Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses. Veterinary Microbiology 2015, 176: 61-69. PMID: 25592760, DOI: 10.1016/j.vetmic.2014.12.024.Peer-Reviewed Original ResearchConceptsGenetic diversityPorcine group C rotavirusPorcine RVCRVC strainsLarge genetic diversityVP6 geneGroup C rotavirusFrequent reassortment eventsStrong geographical differencesPhylogenetic dataPhylogenetic analysisHost speciesHuman RVCORF sequencesC rotavirusReassortment eventsGene segmentsPig industryG7 genotypeImportant pathogenGenesDiversityEconomic impactVP4 sequencesDefinitive cut
2014
Comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves
Park J, Kim D, Matthijnssens J, Kwon H, Zeller M, Alfajaro M, Son K, Hosmillo M, Ryu E, Kim J, Lee J, Park S, Kang M, Kwon J, Choi J, Cho K. Comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. Veterinary Microbiology 2014, 172: 51-62. PMID: 24861840, DOI: 10.1016/j.vetmic.2014.04.010.Peer-Reviewed Original ResearchConceptsHost range restrictionGenotype constellationHistopathological changesCritical molecular determinantsSmall intestineReassortant rotavirusesComparison of pathogenicityDiarrheic pigletsPathological phenotypesDifferent virulenceReassortant strainsPigletsCalvesNSP4 genesSpecific mutationsAmino acid substitutionsRotavirus evolutionMolecular determinantsNSP3 genePathogenicityVirulenceAcid substitutionsNucleotide changesDiarrheaRotavirusAnti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract
Alfajaro M, Rho M, Kim H, Park J, Kim D, Hosmillo M, Son K, Lee J, Park S, Kang M, Ryu Y, Park K, Oh H, Lee S, Park S, Lee W, Cho K. Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Research In Veterinary Science 2014, 96: 567-575. PMID: 24704033, DOI: 10.1016/j.rvsc.2014.03.011.Peer-Reviewed Original ResearchConceptsCombination therapyAnti-rotaviral activityAnti-rotavirus effectIntestinal lesion scoresLethal dose 50Rotavirus enteritisCurative medicationsRotaviral enteritisSophora flavescensRotaviral diarrheaOrgan weightsPiglet modelPathological changesFecal virusBody weightTherapyVirus replicationLesion scoresSingle treatmentDose 50Porcine rotavirusAdverse effectsPreliminary dataDiarrheaEnteritis
2013
Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves
Park J, Kim H, Matthijnssens J, Alfajaro M, Kim D, Son K, Kwon H, Hosmillo M, Ryu E, Kim J, Cena R, Lee J, Kang M, Park S, Cho K. Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves. Veterinary Research 2013, 44: 88. PMID: 24083947, PMCID: PMC3851489, DOI: 10.1186/1297-9716-44-88.Peer-Reviewed Original ResearchConceptsHistopathological changesSmall intestineGroup A rotavirusesDirect interspecies transmissionGenotype constellationCritical molecular determinantsFull-length sequencing analysisA rotavirusesBovine rotavirusVP1-VP4Pathological phenotypesDifferent virulencePigletsDiarrheaInterspecies transmissionAmino acid mutationsRotavirusCalvesIntestineSpecific mutationsTropismMolecular determinantsSequencing analysisUntranslated regionPorcine
2012
Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea
Alfajaro M, Kim H, Park J, Ryu E, Kim J, Jeong Y, Kim D, Hosmillo M, Son K, Lee J, Kwon H, Ryu Y, Park S, Park S, Lee W, Cho K. Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea. Virology Journal 2012, 9: 310. PMID: 23244491, PMCID: PMC3547719, DOI: 10.1186/1743-422x-9-310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAnti-Inflammatory Agents, Non-SteroidalAntiviral AgentsCell LineColostrumDiarrheaDose-Response Relationship, DrugDrug Evaluation, PreclinicalFecesGlycyrrhiza uralensisInterleukin-8Intestine, SmallMAP Kinase Signaling SystemModels, AnimalNF-kappa BPhytotherapyPlant ExtractsPlant RootsRNA, MessengerRotavirusRotavirus InfectionsSpleenSwineSwine DiseasesTumor Necrosis Factor-alphaVirus SheddingConceptsInflammation-related cytokinesGlycyrrhiza uralensis extractMRNA expression levelsSmall intestineRotavirus diarrheaRotaviral enteritisAnti-rotaviral effectAnti-inflammatory effectsFecal virus sheddingOnset of diarrheaColostrum-deprived pigletsExpression levelsIntestinal lesion scoresFecal consistency scoreAntiviral efficacyVirus sheddingHistological changesSevere gastroenteritisFecal virusAntiviral agentsDiarrheaLesion scoresViable medicationsHerbal medicineAntiviral propertiesPathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets
Kim H, Park J, Alfajaro M, Kim D, Hosmillo M, Son K, Lee J, Bae Y, Park S, Kang M, Cho K. Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets. Veterinary Microbiology 2012, 159: 11-22. PMID: 22465801, DOI: 10.1016/j.vetmic.2012.03.017.Peer-Reviewed Original ResearchConceptsExtra-intestinal organsMesenteric lymph nodesCross-species pathogenicityReassortant rotavirusesLymph nodesIntestinal pathologyPersistent severe diarrheaSevere intestinal pathologyExtra-intestinal infectionsDiarrhea fecal samplesReal-time RT-PCRGreen real-time RT-PCRSYBR Green real-time RT-PCRSevere diarrheaCerebrospinal fluidChoroid plexusViral replicationRotavirusRT-PCRViral RNAFecal samplesPigletsDiarrheaPublic healthPathology
2011
Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets
Kim H, Park J, Matthijnssens J, Lee J, Bae Y, Alfajaro M, Park S, Kang M, Cho K. Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. Veterinary Microbiology 2011, 152: 291-303. PMID: 21658866, DOI: 10.1016/j.vetmic.2011.05.017.Peer-Reviewed Original ResearchConceptsGroup A rotavirusesExtra-intestinal spreadCross-species pathogenicityPost-inoculation day 1Intestinal villous atrophyVirus-inoculated calvesExtra-intestinal organsMesenteric lymph nodesBovine group A rotavirusesColostrum-deprived calvesViral RNAReal-time RT-PCRNSP4 gene segmentsGreen real-time RT-PCRSYBR Green real-time RT-PCRVillous atrophyLymph nodesClinical symptomsGene segmentsSevere diarrheaCerebrospinal fluidChoroid plexusDay 1Viral replicationReassortant rotavirusesReassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in South Korea
Park S, Matthijnssens J, Saif L, Kim H, Park J, Alfajaro M, Kim D, Son K, Yang D, Hyun B, Kang M, Cho K. Reassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in South Korea. Veterinary Microbiology 2011, 152: 55-66. PMID: 21592683, DOI: 10.1016/j.vetmic.2011.04.015.Peer-Reviewed Original ResearchConceptsGenotype constellationT1-E1-H1 genotypesH1 genotype constellationFull-length ORFDetailed phylogenetic analysisGroup A rotavirusesC1-M2Phylogenetic analysisComplete genomeN2-T6Genome constellationM2-A3VP1-4I5-R1Bovine group A rotavirusesHeterologous speciesI2-R2A1-N1ReassortmentG8 genotypeSingle strainReassortant strainsGenotypesStrainsGenome