2015
Acetylcholine Acts through Nicotinic Receptors to Enhance the Firing Rate of a Subset of Hypocretin Neurons in the Mouse Hypothalamus through Distinct Presynaptic and Postsynaptic Mechanisms1,2
Zhou WL, Gao XB, Picciotto MR. Acetylcholine Acts through Nicotinic Receptors to Enhance the Firing Rate of a Subset of Hypocretin Neurons in the Mouse Hypothalamus through Distinct Presynaptic and Postsynaptic Mechanisms1,2. ENeuro 2015, 2: eneuro.0052-14.2015. PMID: 26322330, PMCID: PMC4551500, DOI: 10.1523/eneuro.0052-14.2015.Peer-Reviewed Original ResearchHypocretin neuronsMiniature EPSCsPostsynaptic nAChRsFiring rateHypocretin/orexin neuronsFrequency of mEPSCsAntagonism of nAChRsPhasic ACh releasePresence of atropineApplication of AChInternal calcium releaseNicotinic acetylcholine receptorsMouse brain slicesOne-thirdAddiction-related behaviorsPresynaptic AChPresynaptic nAChRsOrexin neuronsACh releaseGlutamatergic inputsPostsynaptic modulationPostsynaptic mechanismsMEPSC frequencyTonic activationBrain slices
2013
Galanin‐induced decreases in nucleus accumbens/striatum excitatory postsynaptic potentials and morphine conditioned place preference require both galanin receptor 1 and galanin receptor 2
Einstein EB, Asaka Y, Yeckel MF, Higley MJ, Picciotto MR. Galanin‐induced decreases in nucleus accumbens/striatum excitatory postsynaptic potentials and morphine conditioned place preference require both galanin receptor 1 and galanin receptor 2. European Journal Of Neuroscience 2013, 37: 1541-1549. PMID: 23387435, PMCID: PMC3648588, DOI: 10.1111/ejn.12151.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic potentialsAbility of galaninSame receptor subtypeReceptor subtypesKnockout miceMorphine CPPPostsynaptic potentialsPlace preferenceAmplitude of EPSPsGalanin receptor 1Galanin receptor subtypesGalanin receptor 2Striatal brain slicesWild-type miceWhole-cell recordingsPotential cellular mechanismsNeuropeptide galaninEPSP amplitudeOpiate rewardBrain slicesNucleus accumbensDorsal striatumExcitatory signalingGalaninReceptor 2
2012
Constitutive knockout of the membrane cytoskeleton protein beta adducin decreases mushroom spine density in the nucleus accumbens but does not prevent spine remodeling in response to cocaine
Jung Y, Mulholland PJ, Wiseman SL, Chandler LJ, Picciotto MR. Constitutive knockout of the membrane cytoskeleton protein beta adducin decreases mushroom spine density in the nucleus accumbens but does not prevent spine remodeling in response to cocaine. European Journal Of Neuroscience 2012, 37: 1-9. PMID: 23106536, PMCID: PMC3538958, DOI: 10.1111/ejn.12037.Peer-Reviewed Original ResearchConceptsNucleus accumbensCocaine administrationSpine densitySynaptic plasticityMature mushroom-shaped spinesMushroom spine densityDaily cocaine administrationMushroom-shaped spinesChronic cocaine administrationOverall spine densityElevated locomotor activityExperience-dependent remodelingLipophilic dye DiINAc slicesWT miceMushroom spinesKO miceCocaine treatmentDiolistic labelingSynaptic remodelingBrain slicesBrain areasConstitutive knockoutMature spinesLocomotor activity
2011
Plasticity of Prefrontal Attention Circuitry: Upregulated Muscarinic Excitability in Response to Decreased Nicotinic Signaling Following Deletion of α5 or β2 Subunits
Tian MK, Bailey CD, De Biasi M, Picciotto MR, Lambe EK. Plasticity of Prefrontal Attention Circuitry: Upregulated Muscarinic Excitability in Response to Decreased Nicotinic Signaling Following Deletion of α5 or β2 Subunits. Journal Of Neuroscience 2011, 31: 16458-16463. PMID: 22072695, PMCID: PMC3240894, DOI: 10.1523/jneurosci.3600-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAconitineAge FactorsAnalysis of VarianceAnimalsAtropineDihydro-beta-ErythroidineIntracellular Signaling Peptides and ProteinsMaleMembrane PotentialsMiceMice, KnockoutNeuronal PlasticityNeuronsNeuropeptidesNeurotransmitter AgentsNicotineNicotinic AntagonistsOrexinsPatch-Clamp TechniquesPrefrontal CortexReceptors, MuscarinicReceptors, NicotinicSignal TransductionUp-RegulationConceptsLayer VI neuronsNicotinic receptorsCholinergic excitationCholinergic receptorsPrefrontal cortexExcitatory muscarinic receptorsPrefrontal attention circuitryMuscarinic cholinergic receptorsMuscarinic acetylcholine receptorsAcute brain slicesWild-type miceWhole-cell recordingsΒ2 subunitNicotinic receptor subunitsMedial prefrontal cortexPyramidal neuronsMuscarinic receptorsNicotinic signalingLayer VIAttention circuitryCholinergic stimulationBrain slicesNicotinic stimulationAcetylcholine receptorsTiming of excitation
2007
Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons
Rao Y, Liu ZW, Borok E, Rabenstein RL, Shanabrough M, Lu M, Picciotto MR, Horvath TL, Gao XB. Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons. Journal Of Clinical Investigation 2007, 117: 4022-4033. PMID: 18060037, PMCID: PMC2104495, DOI: 10.1172/jci32829.Peer-Reviewed Original ResearchConceptsHypocretin/orexin neuronsLong-term potentiationOrexin neuronsGlutamatergic synapsesSynaptic plasticitySleep lossExperience-dependent synaptic plasticityDopamine D1 receptorsChronic sleep lossSleep-wake regulationModafinil treatmentLateral hypothalamusD1 receptorsSimilar potentiationBrain slicesNeuronal activityNeuronal circuitryDopamine systemNervous systemSynaptic strengthNeuronsPathological conditionsGentle handlingMiceWakefulness
1998
Identification of Four Classes of Brain Nicotinic Receptors Using β2 Mutant Mice
Zoli M, Léna C, Picciotto M, Changeux J. Identification of Four Classes of Brain Nicotinic Receptors Using β2 Mutant Mice. Journal Of Neuroscience 1998, 18: 4461-4472. PMID: 9614223, PMCID: PMC6792706, DOI: 10.1523/jneurosci.18-12-04461.1998.Peer-Reviewed Original ResearchConceptsBeta2-/- miceElectrophysiological experimentsBrain regionsNeuronal nicotinic acetylcholine receptor subunitBrain nicotinic receptorsNicotinic acetylcholine receptor subunitsMost brain regionsPatch-clamp recordingsReceptor autoradiography studiesOrder of potencyDifferent brain areasAcetylcholine receptor subunitsThin brain slicesDifferent brain regionsIndividual alpha subunitsAutoradiography studiesBrain slicesNicotinic receptorsNicotinic agonistsHigh affinitySubunit compositionBrain areasAlpha subunitAlpha7 subunitType 2
1995
Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain
Picciotto M, Zoli M, Léna C, Bessis A, Lallemand Y, LeNovère N, Vincent P, Pich E, Brûlet P, Changeux J. Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain. Nature 1995, 374: 65-67. PMID: 7870173, DOI: 10.1038/374065a0.Peer-Reviewed Original ResearchConceptsHigh-affinity nicotine receptorsNeuronal nicotinic acetylcholine receptorsBrains of miceΒ2-/- miceNicotinic acetylcholine receptorsThalamic neuronsNicotine applicationFunctional nAChRsNicotine receptorsBrain slicesNicotinic subunitsAbnormal avoidanceAcetylcholine receptorsAspects of behaviorHigh-affinity binding sitesMutant miceElectrophysiological recordingsPassive avoidanceAssociative memoryMiceNicotineNeuronal nicotinic subunitsNon-mutant siblingsBrainReceptors