2020
High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing
Landry ML, Topal JE, Estis J, Katzenbach P, Nolan N, Sandlund J. High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing. Journal Of Clinical Microbiology 2020, 58: 10.1128/jcm.01629-19. PMID: 31776192, PMCID: PMC6989068, DOI: 10.1128/jcm.01629-19.Peer-Reviewed Original ResearchConceptsToxin enzyme immunoassayToxin A/BCell cytotoxicity neutralization assayEnzyme immunoassayStool samplesDifficile toxin assaysProspective clinical studyCytotoxicity neutralization assayCare algorithmChart reviewClinical studiesToxin ACytotoxin testingNeutralization assaysToxin assaysNegative agreementDiscordant samplesLaboratory algorithmTesting algorithmHigh agreementInfection diagnosticsGlutamate dehydrogenasePositive agreementImmunoassayAssays
2013
Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
Landry ML, Ferguson D, Topal J. Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease. Journal Of Clinical Microbiology 2013, 52: 275-280. PMID: 24226924, PMCID: PMC3911468, DOI: 10.1128/jcm.02545-13.Peer-Reviewed Original ResearchConceptsAntigen-negative samplesGDH antigenToxigenic C. difficileC. difficileGDH-positive samplesClostridium difficile infectionGlutamate dehydrogenase antigenToxin B geneSemisolid stoolsC. difficile toxin B geneDifficile testingChart reviewNosocomial transmissionDifficile infectionDifficile diseaseReal-time PCRClinical consequencesMerit further studyRapid molecular diagnosisDifficile bacteriaAntigenPatientsDehydrogenase antigenCost avoidanceCytotoxicity assays
2009
Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
Kvach EJ, Ferguson D, Riska PF, Landry ML. Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection. Journal Of Clinical Microbiology 2009, 48: 109-114. PMID: 19864479, PMCID: PMC2812270, DOI: 10.1128/jcm.01630-09.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsBacterial ToxinsBacteriological TechniquesClinical Laboratory TechniquesClostridioides difficileEnterocolitis, PseudomembranousEnterotoxinsEnzyme-Linked Immunosorbent AssayGlutamate DehydrogenaseHumansNeutralization TestsPolymerase Chain ReactionReagent Kits, DiagnosticSensitivity and Specificity
2001
Evaluation of Biosite Triage Clostridium difficile Panel for Rapid Detection of Clostridium difficile in Stool Samples
Landry M, Topal J, Ferguson D, Giudetti D, Tang Y. Evaluation of Biosite Triage Clostridium difficile Panel for Rapid Detection of Clostridium difficile in Stool Samples. Journal Of Clinical Microbiology 2001, 39: 1855-1858. PMID: 11326003, PMCID: PMC88038, DOI: 10.1128/jcm.39.5.1855-1858.2001.Peer-Reviewed Original ResearchConceptsNegative predictive valuePositive predictive valueCommon antigenStool samplesPredictive valueC. difficile toxin AHigh negative predictive valueAntigen-positive patientsDifficile-negative patientsAntigen positive resultDifficile toxin ADifficile carriersContact precautionsAntibiotic treatmentToxin ACytotoxin titersTriage PanelUnnecessary useClostridium difficilePatients