2013
Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
Landry ML, Ferguson D, Topal J. Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease. Journal Of Clinical Microbiology 2013, 52: 275-280. PMID: 24226924, PMCID: PMC3911468, DOI: 10.1128/jcm.02545-13.Peer-Reviewed Original ResearchConceptsAntigen-negative samplesGDH antigenToxigenic C. difficileC. difficileGDH-positive samplesClostridium difficile infectionGlutamate dehydrogenase antigenToxin B geneSemisolid stoolsC. difficile toxin B geneDifficile testingChart reviewNosocomial transmissionDifficile infectionDifficile diseaseReal-time PCRClinical consequencesMerit further studyRapid molecular diagnosisDifficile bacteriaAntigenPatientsDehydrogenase antigenCost avoidanceCytotoxicity assays
2009
Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
Kvach EJ, Ferguson D, Riska PF, Landry ML. Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection. Journal Of Clinical Microbiology 2009, 48: 109-114. PMID: 19864479, PMCID: PMC2812270, DOI: 10.1128/jcm.01630-09.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsBacterial ToxinsBacteriological TechniquesClinical Laboratory TechniquesClostridioides difficileEnterocolitis, PseudomembranousEnterotoxinsEnzyme-Linked Immunosorbent AssayGlutamate DehydrogenaseHumansNeutralization TestsPolymerase Chain ReactionReagent Kits, DiagnosticSensitivity and Specificity