2023
Use of experimental medicine approaches for the development of novel psychiatric treatments based on orexin receptor modulation
Beckenstrom A, Coloma P, Dawson G, Finlayson A, Malik A, Post A, Steiner M, Potenza M. Use of experimental medicine approaches for the development of novel psychiatric treatments based on orexin receptor modulation. Neuroscience & Biobehavioral Reviews 2023, 147: 105107. PMID: 36828161, PMCID: PMC10165155, DOI: 10.1016/j.neubiorev.2023.105107.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsExperimental medicine approachPerformance outcome measuresPsychiatric disordersMedicine approachReal-world clinical practiceOrexin neuropeptide systemMarkers of efficacyPatient advocacy groupsOrexin modulationReceptor modulationSymptom evaluationReceptor modulatorsClinical studiesMood disordersPreclinical studiesNeuropeptide systemsPsychiatric treatmentAnimal modelsPathological mechanismsClinical practiceClinical researchClinical useBrain functionTranslational researchPotential target
1994
Functional expression and characterization of human D2 and D3 dopamine receptors
Potenza M, Graminski G, Schmauss C, Lerner. Functional expression and characterization of human D2 and D3 dopamine receptors. Journal Of Neuroscience 1994, 14: 1463-1476. PMID: 7907363, PMCID: PMC6577580, DOI: 10.1523/jneurosci.14-03-01463.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological AssayCyclic AMPDopamine AgentsDopamine AntagonistsHumansLigandsMelanocyte-Stimulating HormonesMelanophoresMelatoninPertussis ToxinPigments, BiologicalReceptors, DopamineReceptors, Dopamine D2Receptors, Dopamine D3TetrahydronaphthalenesVirulence Factors, BordetellaXenopus laevisConceptsD3 receptorsCAMP accumulationGi protein-coupled receptorHuman D2Selective dopaminergic agonistsDose-dependent mannerIC50 value determinationDopaminergic agonistsMelanocyte receptorsPertussis toxinD3 dopamineAgonistsReceptorsPigment granule aggregationAntagonistD2RXenopus laevis melanophoresSimilar concentrationsFunctional expressionPigment aggregationD3R.Initial feasibility studyFunctional characteristicsCultured Xenopus laevis melanophoresNew bioassayCharacterization of a serotonin receptor endogenous to frog melanophores
Potenza M, Lerner M. Characterization of a serotonin receptor endogenous to frog melanophores. Naunyn-Schmiedeberg's Archives Of Pharmacology 1994, 349: 11-19. PMID: 8139699, DOI: 10.1007/bf00178200.Peer-Reviewed Original Research
1993
A New Tool for Investigating G Protein‐Coupled Receptors
Lerner M, Potenza M, Graminski G, Mcclintock T, Jayawickreme C, Karne S. A New Tool for Investigating G Protein‐Coupled Receptors. Novartis Foundation Symposia 1993, 179: 76-96. PMID: 8168384, DOI: 10.1002/9780470514511.ch6.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsProtein-coupled receptorsIntracellular signal transduction pathwaysVertebrate olfactory receptorsSignal transduction pathwaysG protein-coupled receptor familyStructure-function analysisProtein-coupled receptor familyCloning of cDNASecond messenger cAMPOdorant receptorsTransduction pathwaysG proteinsOlfactory receptorsFunctional interactionReceptor familyReceptor couplingReceptorsCloningCDNANew toolProteinDiacylglycerolTrisphosphateInositol
1992
A Rapid Quantitative Bioassay for Evaluating the Effects of Ligands Upon Receptors That Modulate cAMP Levels in a Melanophore Cell Line
POTENZA M, LERNER M. A Rapid Quantitative Bioassay for Evaluating the Effects of Ligands Upon Receptors That Modulate cAMP Levels in a Melanophore Cell Line. Pigment Cell & Melanoma Research 1992, 5: 372-378. PMID: 1337205, DOI: 10.1111/j.1600-0749.1992.tb00565.x.Peer-Reviewed Original ResearchConceptsDose-dependent pigment dispersionCell linesBeta-adrenergic receptorsEffects of antagonistsMelanophore cell lineEffects of drugsDose-response curveReproducible dose-response curvesAdrenergic agonistsAdrenergic receptorsIntracellular cAMPCAMP levelsReceptorsSubsequent exposureTime-course analysisXenopus laevis melanophoresA method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay
Potenza M, Graminski G, Lerner M. A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay. Analytical Biochemistry 1992, 206: 315-322. PMID: 1332547, DOI: 10.1016/0003-2697(92)90372-e.Peer-Reviewed Original ResearchConceptsBeta-2 adrenergic receptorsAdrenergic receptorsGs protein-coupled receptorsEffects of drugsDose-response curveHuman beta 2 adrenergic receptorRank order potencyProtein-coupled receptorsReproducible dose-response curvesPharmacological characterizationConcomitant elevationStimulatory guaninePigment dispersionAgonistsIntracellular cAMPImportant receptorReceptorsAntagonistSpecific GsIC50 valuesFunctional expressionCellsXenopus laevis
1991
A Recombinant Vaccinia Virus Infects Xenopus Melanophores
Potenza M, Lerner M. A Recombinant Vaccinia Virus Infects Xenopus Melanophores. Pigment Cell & Melanoma Research 1991, 4: 186-192. PMID: 1816550, DOI: 10.1111/j.1600-0749.1991.tb00437.x.Peer-Reviewed Original ResearchConceptsXenopus cellsWestern blot analysisXenopus melanophoresMultiplicity of infectionCultured Xenopus laevis melanophoresHuman growth hormone geneBlot analysisGrowth hormone geneVirus/host interactionsBeta-galactosidase productionTranscriptional controlAmphibian cellsXenopus laevis melanophoresAmplification of virusPigment cellsLate proteinsViral promotersEfficient expressionHormone geneHost interactionsVaccinia virusLac ZEfficient replication