2015
The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Perkins LA, Holderbaum L, Tao R, Hu Y, Sopko R, McCall K, Yang-Zhou D, Flockhart I, Binari R, Shim HS, Miller A, Housden A, Foos M, Randkelv S, Kelley C, Namgyal P, Villalta C, Liu LP, Jiang X, Huan-Huan Q, Wang X, Fujiyama A, Toyoda A, Ayers K, Blum A, Czech B, Neumuller R, Yan D, Cavallaro A, Hibbard K, Hall D, Cooley L, Hannon GJ, Lehmann R, Parks A, Mohr SE, Ueda R, Kondo S, Ni JQ, Perrimon N. The Transgenic RNAi Project at Harvard Medical School: Resources and Validation. Genetics 2015, 201: 843-852. PMID: 26320097, PMCID: PMC4649654, DOI: 10.1534/genetics.115.180208.Peer-Reviewed Original Research
2005
Drosophila myosin V is required for larval development and spermatid individualization
Mermall V, Bonafé N, Jones L, Sellers JR, Cooley L, Mooseker MS. Drosophila myosin V is required for larval development and spermatid individualization. Developmental Biology 2005, 286: 238-255. PMID: 16126191, DOI: 10.1016/j.ydbio.2005.07.028.Peer-Reviewed Original ResearchConceptsInvestment conesLarval developmentClass V myosinsIndividualization complexSpermatid individualizationCytological defectsTruncation alleleVesicular trafficRNA transportActin structuresLarval tissuesMutant animalsMature spermSperm nucleiMyoVSpermatid maturationMolecular motorsMyosin VMechanochemical couplingDetectable defectsV geneMicrotubulesIndividual membranesActinSpermatogenesis
2002
Mutations in the midway Gene Disrupt a Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase
Buszczak M, Lu X, Segraves WA, Chang TY, Cooley L. Mutations in the midway Gene Disrupt a Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase. Genetics 2002, 160: 1511-1518. PMID: 11973306, PMCID: PMC1462074, DOI: 10.1093/genetics/160.4.1511.Peer-Reviewed Original ResearchConceptsEgg chambersDiacylglycerol acyltransferaseNurse cellsAcyl coenzyme AMutant egg chambersNurse cell deathCell deathInsect cells resultsEgg chamber developmentCoenzyme AGermline apoptosisDrosophila oogenesisCytoplasm transportDGAT activityCells resultsChamber developmentNeutral lipidsGenesLipid metabolismDiacylglycerolApoptosisAcyltransferaseDrosophilaCellsOogenesisA subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex
Hudson AM, Cooley L. A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex. Journal Of Cell Biology 2002, 156: 677-687. PMID: 11854308, PMCID: PMC2174088, DOI: 10.1083/jcb.200109065.Peer-Reviewed Original ResearchConceptsArp2/3 complexRing canal growthActin-related proteinsParallel actin bundlesNurse cell cytoplasmActin filament nucleationDynamic actin rearrangementsActin cytoskeletonRing canalsActin structuresSlow spontaneous rateActin rearrangementPupal epitheliumPlasma membraneFilament nucleationShaft cellsActin bundlesActin filamentsComplex contributesFunction mutationsCanal growthCell cytoplasmSubunitsMutationsComplexesSCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila
Zallen JA, Cohen Y, Hudson AM, Cooley L, Wieschaus E, Schejter ED. SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila. Journal Of Cell Biology 2002, 156: 689-701. PMID: 11854309, PMCID: PMC2174092, DOI: 10.1083/jcb.200109057.Peer-Reviewed Original ResearchMeSH KeywordsActin-Related Protein 2Actin-Related Protein 3ActinsAmino Acid SequenceAnimalsAxonsBase SequenceBlastodermBrainCytoplasmCytoskeletal ProteinsDNA, ComplementaryDrosophilaDrosophila ProteinsGenes, InsectHumansInsect ProteinsMicrofilament ProteinsMolecular Sequence DataMorphogenesisMutagenesisOogenesisOvumProteinsSequence Homology, Amino AcidWiskott-Aldrich Syndrome ProteinConceptsWiskott-Aldrich syndrome proteinArp2/3 complexAdult eye morphologyScar/WAVECell fate decisionsActin-rich structuresCell biological eventsCortical filamentous actinCell morphologyDrosophila developmentMultiple cell typesNormal cell morphologySCAR homologueFate decisionsSyndrome proteinActin structuresFilamentous actinActin polymerizationCell shapeMorphological eventsCytoplasmic organizationEye morphologyBiological eventsCell typesDevelopmental requirements
1999
Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila
Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development 1999, 126: 4581-4589. PMID: 10498692, DOI: 10.1242/dev.126.20.4581.Peer-Reviewed Original ResearchConceptsEgg chamber developmentGermline clonesResponse genesGene expressionDorsal follicle cell fatesEgg chamber maturationMutant egg chambersFollicle cell fatesSteroid hormone ecdysoneEcdysone response genesProgression of oogenesisTemperature-sensitive mutationStage-specific mannerTemporal expression profilesEarly response genesChamber developmentEcdysone responsivenessChamber maturationHormone ecdysoneGenetic hierarchyEcR expressionDrosophila melanogasterEgg chambersCell fateEarly gene expression
1998
Apoptosis in late stage Drosophila nurse cells does not require genes within the H99 deficiency
Foley K, Cooley L. Apoptosis in late stage Drosophila nurse cells does not require genes within the H99 deficiency. Development 1998, 125: 1075-1082. PMID: 9463354, DOI: 10.1242/dev.125.6.1075.Peer-Reviewed Original ResearchConceptsEgg chambersNurse cellsDNA fragmentationDrosophila nurse cellsMutant egg chambersDrosophila egg chamberOvarian expression patternsDrosophila apoptosisGermline clonesHead involutionCytoplasm transportPositive regulatorRegulatory genesStage 13Cytoplasm transferApoptotic vesiclesCytoplasmic factorsNegative regulatorExpression patternsWild typeGenesFragmented DNAOogenesisApoptosisStage 12
1997
Formation of the Drosophila Ovarian Ring Canal Inner Rim Depends on cheerio
Robinson D, Smith-Leiker T, Sokol N, Hudson A, Cooley L. Formation of the Drosophila Ovarian Ring Canal Inner Rim Depends on cheerio. Genetics 1997, 145: 1063-1072. PMID: 9093858, PMCID: PMC1207876, DOI: 10.1093/genetics/145.4.1063.Peer-Reviewed Original ResearchMeSH KeywordsActinsAllelesAnimalsCalmodulin-Binding ProteinsCarrier ProteinsCell CommunicationCell MembraneChromosome MappingCytoskeletonDrosophila melanogasterDrosophila ProteinsFemaleGene Expression Regulation, DevelopmentalGenes, InsectInfertility, FemaleInsect ProteinsIntercellular JunctionsMicrofilament ProteinsOocytesOvaryConceptsStable intercellular bridgesExamination of mutantsDrosophila oogenesisPlasma membrane stabilizationRing canalsCytoplasm transportMutant cellsFilamentous actinCleavage furrowRIM proteinsNurse cellsActin filamentsIntercellular bridgesMutantsCritical functionsKelchCheeriosProteinStep-wise processAssemblyMembrane stabilizationCellsCytoskeletonOogenesisGenes
1996
Single Amino Acid Mutations in Drosophila Fascin Disrupt Actin Bundling Function in Vivo
Cant K, Cooley L. Single Amino Acid Mutations in Drosophila Fascin Disrupt Actin Bundling Function in Vivo. Genetics 1996, 143: 249-258. PMID: 8722779, PMCID: PMC1207258, DOI: 10.1093/genetics/143.1.249.Peer-Reviewed Original ResearchConceptsEMS mutagenesis screenMutagenesis screenCytoplasm transportActin-bundling functionDiverse cellular processesIntragenic suppressor mutationsBundles actin filamentsCytoplasmic actin bundlesSingle amino acid mutationSerine 289Glutamic acid resultsAmino acid mutationsDominant suppressorsFascin functionFemale sterileSuppressor mutationsCellular processesC-terminusActin bundlesCentral domainActin filamentsSevere defectsMicrovillar projectionsAcid mutationsFilopodial extensions
1994
Intercellular Cytoplasm Transport during Drosophila Oogenesis
Mahajan-Miklos S, Cooley L. Intercellular Cytoplasm Transport during Drosophila Oogenesis. Developmental Biology 1994, 165: 336-351. PMID: 7958404, DOI: 10.1006/dbio.1994.1257.Peer-Reviewed Original ResearchMorphogenesis of Drosophila ovarian ring canals
Robinson D, Cant K, Cooley L. Morphogenesis of Drosophila ovarian ring canals. Development 1994, 120: 2015-2025. PMID: 7925006, DOI: 10.1242/dev.120.7.2015.Peer-Reviewed Original ResearchConceptsRing canalsEgg chambersHu-li tai shaoMutant egg chambersOvarian ring canalsEgg chamber developmentDrosophila egg chamberCanal developmentCarboxy-terminal tailCytoskeletal protein assemblyAnti-phosphotyrosine antibodyProtein assembliesHT proteinsTyrosine phosphorylationFilamentous actinCytoplasmic bridgesActinChamber developmentKelchProteinCanal componentsAssemblyMorphogenesisGermariumPhosphorylationThe villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis
Mahajan-Miklos S, Cooley L. The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis. Cell 1994, 78: 291-301. PMID: 8044841, DOI: 10.1016/0092-8674(94)90298-4.Peer-Reviewed Original ResearchConceptsVillin-like proteinNurse cellsActin filament bundlesQuail geneMutant egg chambersActin bundle assemblyFilament bundlesEgg chambersFemale sterilityAdult fliesCytoplasmic transportFilamentous actinGene resultsBundle assemblyActin filamentsQuail proteinProtein villinAbsorptive epithelial cellsStriking colocalizationProteinOogenesisVillinEpithelial cellsGenesCellsProfilin mutations disrupt multiple actin-dependent processes during Drosophila development
Verheyen E, Cooley L. Profilin mutations disrupt multiple actin-dependent processes during Drosophila development. Development 1994, 120: 717-728. PMID: 7600952, DOI: 10.1242/dev.120.4.717.Peer-Reviewed Original ResearchConceptsSmall actin binding proteinsEmbryonic lethal phenotypeActin-dependent processesActin binding proteinsActin filament bundlesDrosophila profilinProfilin mutationDrosophila developmentMulticellular organismsViable allelesBristle formationLethal phenotypeActin assemblyProfilin functionSubcellular localizationGenomic deletionsBinding proteinCell migrationCell typesProfilinFilament bundlesOogenesisAbnormal regulationBinucleate cellsDeletion