Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene.
Cooley L, Schaack J, Burke DJ, Thomas B, Söll D. Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene. Molecular And Cellular Biology 1984, 4: 2714-2722. PMID: 6570190, PMCID: PMC369281, DOI: 10.1128/mcb.4.12.2714.Peer-Reviewed Original ResearchConceptsDrosophila Kc cell extractHeLa cell extractsCell extractsReal genesStable complex formationControl regionDeletion analysisStable transcription complex formationRecombinant clonesDrosophila tRNAArg geneTRNA gene clusterTranscription complex formationBona fide genesInternal control regionTranscription factor bindingSame DNA strandComplex formationTranscription control regionsConsecutive base pairsTRNAHis geneTRNAArg geneFide genesGene clusterTranscription factorsFactor bindingTranscriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster
Sharp S, Garcia A, Cooley L, Söll D. Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster. Nucleic Acids Research 1984, 12: 7617-7632. PMID: 6093044, PMCID: PMC320189, DOI: 10.1093/nar/12.20.7617.Peer-Reviewed Original ResearchConceptsEfficiency of transcriptionRRNA gene copiesHigh transcription efficiencyTwo-nucleotide deletionD. melanogasterGene repeatRNA genesGene clusterPrimary transcriptGene copiesTranscription functionTranscription efficiencyTemplate activityCell extractsTranscriptionDNAPosition 28Position 86DNA typesRepeat unitsDeletionSame sequenceMelanogasterRRNAGenesThe extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation.
Schaack J, Sharp S, Dingermann T, Burke DJ, Cooley L, Söll D. The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation. Journal Of Biological Chemistry 1984, 259: 1461-1467. PMID: 6693417, DOI: 10.1016/s0021-9258(17)43429-6.Peer-Reviewed Original ResearchConceptsStable complex formationBase pairsDrosophila Kc cell extractSequence requirementsCell extractsEukaryotic tRNA genesStable transcription complexesHeLa cell extractsTRNA genesComplex formationTranscription complexArg genesEfficient transcriptionTranscription assaysTranscription propertiesCell-free extractsTranscriptionHomologous systemGenesSequenceSequence dependenceCellular sourceExtractAssaysPairs