2016
Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectBiaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix content
2014
Influence of pH on Extracellular Matrix Preservation During Lung Decellularization
Tsuchiya T, Balestrini JL, Mendez J, Calle EA, Zhao L, Niklason LE. Influence of pH on Extracellular Matrix Preservation During Lung Decellularization. Tissue Engineering Part C Methods 2014, 20: 1028-1036. PMID: 24735501, PMCID: PMC4241865, DOI: 10.1089/ten.tec.2013.0492.Peer-Reviewed Original Research
2010
Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering
Petersen TH, Hitchcock T, Muto A, Calle EA, Zhao L, Gong Z, Gui L, Dardik A, Bowles DE, Counter CM, Niklason LE. Utility of Telomerase-pot1 Fusion Protein in Vascular Tissue Engineering. Cell Transplantation 2010, 19: 79-87. PMID: 19878625, PMCID: PMC2850951, DOI: 10.3727/096368909x478650.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAdultAnimalsBioreactorsBlood VesselsCell Culture TechniquesCells, CulturedCellular SenescenceCollagenGenetic VectorsGraft SurvivalHumansMaleMuscle, Smooth, VascularRatsRats, NudeRecombinant Fusion ProteinsShelterin ComplexTelomeraseTelomere-Binding ProteinsTissue EngineeringTransfectionConceptsTransient deliveryVascular tissue engineeringRegenerative medicineTissue engineeringRegenerative medicine applicationsTissue-engineered constructsLentiviral vectorsMedicine applicationsImportant stumbling blockTelomeric repeat amplification protocolElderly human donorsBetter performanceAmplification protocolEngineeringDeliveryTransient reconstitutionDifferentiated cellsAdenoviral deliveryRepeat amplification protocolFusion proteinTransgeneHuman smooth muscle cellsStumbling blockGreater collagen contentProtocol