2024
Hebbian instruction of axonal connectivity by endogenous correlated spontaneous activity
Matsumoto N, Barson D, Liang L, Crair M. Hebbian instruction of axonal connectivity by endogenous correlated spontaneous activity. Science 2024, 385: eadh7814. PMID: 39146415, DOI: 10.1126/science.adh7814.Peer-Reviewed Original ResearchConceptsSpontaneous activitySpontaneous retinal wavesAxonal connectionsPatterns of correlated activityNeonatal miceEvidence in vivoRetinal wavesPostsynaptic neuronsNeuronal activityIn vivoAxonal arborsAxonal processesAxonsRetinocollicular axonsNeural connectionsIndividual axonsMorphological changesSubcellular precisionEndogenous pattern
2022
Brainstem serotonin neurons selectively gate retinal information flow to thalamus
Reggiani J, Jiang Q, Barbini M, Lutas A, Liang L, Fernando J, Deng F, Wan J, Li Y, Chen C, Andermann M. Brainstem serotonin neurons selectively gate retinal information flow to thalamus. Neuron 2022, 111: 711-726.e11. PMID: 36584680, PMCID: PMC10131437, DOI: 10.1016/j.neuron.2022.12.006.Peer-Reviewed Original ResearchConceptsVisual information streamsRetinal ganglion cell typesBrainstem serotonin neuronsFiber photometry recordingsGanglion cell typesTwo-photon calcium imagingSerotonin neuronsVisual thalamusGlutamate releaseSerotonergic axonsRGC axonsSerotonin axonsThalamocortical neuronsAxon stimulationAwake micePresynaptic terminalsCalcium imagingOptogenetic stimulationCalcium activityLocal visual stimuliAxonsThalamusBoutonsVisual stimuliNeuronsMating-driven variability in olfactory local interneuron wiring
Chou YH, Yang CJ, Huang HW, Liou NF, Panganiban MR, Luginbuhl D, Yin Y, Taisz I, Liang L, Jefferis GSXE, Luo L. Mating-driven variability in olfactory local interneuron wiring. Science Advances 2022, 8: eabm7723. PMID: 35179957, PMCID: PMC8856614, DOI: 10.1126/sciadv.abm7723.Peer-Reviewed Original Research
2020
Organization, Function, and Development of the Mouse Retinogeniculate Synapse
Liang L, Chen C. Organization, Function, and Development of the Mouse Retinogeniculate Synapse. Annual Review Of Vision Science 2020, 6: 261-285. PMID: 32936733, DOI: 10.1146/annurev-vision-121219-081753.Peer-Reviewed Original ResearchConceptsRetinogeniculate synapseDistinct retinal ganglion cell typesRetinal ganglion cell typesGanglion cell typesImage-forming pathwayVisual thalamusThalamocortical neuronsVisual pathwayExperimental modelCircuit organizationThalamusSynapseMiceCell typesFunctional organizationRecent studiesPowerful experimental modelCurrent understandingVisual spaceVisual experiencePathwayNeuronsRGCsRetinal Inputs to the Thalamus Are Selectively Gated by Arousal
Liang L, Fratzl A, Reggiani JDS, El Mansour O, Chen C, Andermann ML. Retinal Inputs to the Thalamus Are Selectively Gated by Arousal. Current Biology 2020, 30: 3923-3934.e9. PMID: 32795442, PMCID: PMC7665906, DOI: 10.1016/j.cub.2020.07.065.Peer-Reviewed Original ResearchConceptsVisual thalamusVisual responsesMajority of boutonsTwo-photon calciumCentral visual processingArousal stateIntermediate spatial frequenciesEnhancement of responsesAxonal boutonsRetinal axonsRetinal inputDifferent pupil sizesVisual pathwayThalamusBoutonsMouse thalamusVisual processingMagnitude of responseVisual information channelsDistinct subsetsEarly stagesPupil sizeNeural activityDifferential modulationBehavioral states
2018
A fine-scale functional logic to convergence from retina to thalamus
Fratzl A, Liang L. A fine-scale functional logic to convergence from retina to thalamus. 2018 DOI: 10.26226/morressier.5b31ec2f2afeeb001345af0c.Peer-Reviewed Original ResearchA Fine-Scale Functional Logic to Convergence from Retina to Thalamus
Liang L, Fratzl A, Goldey G, Ramesh RN, Sugden AU, Morgan JL, Chen C, Andermann ML. A Fine-Scale Functional Logic to Convergence from Retina to Thalamus. Cell 2018, 173: 1343-1355.e24. PMID: 29856953, PMCID: PMC6003778, DOI: 10.1016/j.cell.2018.04.041.Peer-Reviewed Original ResearchConceptsAxonal boutonsRetinal ganglion cellsTwo-photon calciumImage-forming visionClusters of boutonsVisual thalamusGanglion cellsRetinal axonsBouton clustersThalamusMouse thalamusIndividual axonsDifferent axonsFeature selectivityBoutonsFine-scale organizationAxonsDendritic domainsUltrastructural analysisRetina
2017
In Vivo Imaging of CNS Injury and Disease
Akassoglou K, Merlini M, Rafalski VA, Real R, Liang L, Jin Y, Dougherty SE, De Paola V, Linden DJ, Misgeld T, Zheng B. In Vivo Imaging of CNS Injury and Disease. Journal Of Neuroscience 2017, 37: 10808-10816. PMID: 29118209, PMCID: PMC5678013, DOI: 10.1523/jneurosci.1826-17.2017.Peer-Reviewed Original ResearchConceptsCNS injuryNeural transplantationGlial responseNeurovascular unitAxonal degenerationSpinal cordMammalian CNSRodent brainCellular responsesInjuryDiseaseVivo imagingImagingOptical imagingNeuroinflammationResponseMini-SymposiumTransplantationGlialCordTherapyCNSDegenerationImportant new insightsBrain
2013
High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity
Sinha S, Liang L, Ho ET, Urbanek KE, Luo L, Baer TM, Schnitzer MJ. High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 18374-18379. PMID: 24167298, PMCID: PMC3832030, DOI: 10.1073/pnas.1216287110.Peer-Reviewed Original ResearchConceptsLive animalsCellular functionsFruit flySmall organismsMushroom bodiesKenyon cellsFliesAntennal lobeLive organismsOrganismsNeuronal plasticityHigh-throughput preparationMouse craniumExperimental throughputFluorescence imagingMicrosurgical openingLaser microsurgeryAntsVivo microscopy studiesNematodesAnimalsTwo-photon microscopySpeciesPhototaxisOptical experimentationGABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons
Liang L, Li Y, Potter CJ, Yizhar O, Deisseroth K, Tsien RW, Luo L. GABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons. Neuron 2013, 79: 917-931. PMID: 24012005, PMCID: PMC3838762, DOI: 10.1016/j.neuron.2013.06.014.Peer-Reviewed Original ResearchConceptsLateral horn neuronsInhibitory projection neuronsInnate olfactory behaviorsInhibitory tonePresynaptic inhibitionDrosophila olfactory systemHigher-order neuronsProjection neuronsAxon terminalsAntennal lobe glomeruliLateral hornConnection specificityDetectable suppressionOlfactory inputOdor responsesNeuronsOlfactory informationOlfactory systemHigher centersParallel inhibitionDifferential effectsDownstream circuitsInhibition motifCircuit motifsInhibitionLinking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting
Joo WJ, Sweeney LB, Liang L, Luo L. Linking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting. Neuron 2013, 78: 673-686. PMID: 23719164, PMCID: PMC3727417, DOI: 10.1016/j.neuron.2013.03.022.Peer-Reviewed Original ResearchConceptsNeural circuit assemblySema-2bCell fateSpecific cell fatesCircuit assemblyORN classesTemporal regulationTrajectory choiceSema-2aAxon bundle formationGenetic analysisAxon targetingAxon-axon interactionsNeuron developmentCellular mechanismsGuidance moleculesORN axonsSemaphorinsSynaptic targetsFateAssemblyTarget selectionPostsynaptic target neuronsAxonal trajectoriesRegulation
2011
Laser microsurgery for two-photon imaging in fruit flies
Sinha S, Liang L, Ho E, Luo L, Baer T, Schnitzer M. Laser microsurgery for two-photon imaging in fruit flies. 2011, nmc6. DOI: 10.1364/ntm.2011.nmc6.Peer-Reviewed Original Research
2010
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis
Potter CJ, Tasic B, Russler EV, Liang L, Luo L. The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Cell 2010, 141: 536-548. PMID: 20434990, PMCID: PMC2883883, DOI: 10.1016/j.cell.2010.02.025.Peer-Reviewed Original ResearchConceptsMARCM analysisQa gene clusterCell division patternsBinary expression systemTransgene expression patternsTransgene expressionGAL4 systemGene functionMosaic analysisGene clusterRegulatory genesMammalian cellsCell divisionDivision patternsLow basal expressionLineage tracingExpression systemExpression patternsNeuronal lineageOlfactory attractionCell growthBasal expressionDrosophilaRepressionExpressionThe olfactory circuit of the fruit fly Drosophila melanogaster
Liang L, Luo L. The olfactory circuit of the fruit fly Drosophila melanogaster. Science China Life Sciences 2010, 53: 472-484. PMID: 20596914, DOI: 10.1007/s11427-010-0099-z.Peer-Reviewed Original Research