2010
Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials
Hickerson RP, Leachman SA, Pho LN, Gonzalez-Gonzalez E, Smith FJ, McLean WH, Contag CH, Leake D, Milstone LM, Kaspar RL. Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials. Journal Of Investigative Dermatology 2010, 131: 1029-1036. PMID: 21191405, DOI: 10.1038/jid.2010.372.Peer-Reviewed Original ResearchConceptsClinical trialsPachyonychia congenitaEnd pointPhase 1b clinical trialClinical end pointsRare skin disorderMolecular end pointsTypes of trialsImmune surveillancePatient-derived keratinocytesSkin disordersSustained inhibitionTreatment resultsRNAi-based therapeuticsRNA interferenceMRNA levelsTrialsTotal RNAHuman skinKeratinocyte culturesMRNAAmplifiable mRNATherapeuticsInhibitorsInhibition
1988
Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy.
Mangin M, Webb A, Dreyer B, Posillico J, Ikeda K, Weir E, Stewart A, Bander N, Milstone L, Barton D. Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 597-601. PMID: 2829195, PMCID: PMC279598, DOI: 10.1073/pnas.85.2.597.Peer-Reviewed Original ResearchConceptsParathyroid hormone-like peptideHumoral hypercalcemiaHormone-like peptideCommon paraneoplastic syndromeTumor-derived peptidesHuman renal carcinomaParaneoplastic syndromeNormal human keratinocytesHuman PTHRenal carcinomaHuman tumorsSyndromeHuman keratinocytesHypercalcemiaMalignancyBlot analysisTumorsRNA blot hybridization analysisPartial N-terminal amino acid sequencePeptidesMRNABlot hybridization analysisMature peptideCarcinomaPTH
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo