2017
An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons
Zhang Y, Ni W, Horwich AL, Kaczmarek LK. An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons. Journal Of Neuroscience 2017, 37: 2258-2265. PMID: 28119399, PMCID: PMC5338764, DOI: 10.1523/jneurosci.3102-16.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaBiophysicsCells, CulturedElectric StimulationEnzyme InhibitorsGanglia, InvertebrateHumansLuminescent ProteinsMembrane PotentialsMicroinjectionsMorpholinosMutationNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedRNA, Small InterferingSodiumSuperoxide Dismutase-1ConceptsAmyotrophic lateral sclerosisSuperoxide dismutase 1Mutant superoxide dismutase 1Potassium currentC-Jun N-terminal kinaseNeuronal excitabilityLateral sclerosisFatal adult-onset neurodegenerative diseaseN-terminal kinaseMutant human Cu/ZnNeuronal developmentDismutase 1Adult-onset neurodegenerative diseaseCurrent-clamp recordingsMotor neuron toxicityOutward potassium currentHuman Cu/ZnWild-type superoxide dismutase 1Neuron toxicityActivity of NaBag cell neuronsClamp recordingsNeuronal functionCell neuronsAction potentials
2007
Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission
Hickman JA, Hardwick JM, Kaczmarek LK, Jonas EA. Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission. Journal Of Neurophysiology 2007, 99: 1515-1522. PMID: 18160428, PMCID: PMC2836590, DOI: 10.1152/jn.00598.2007.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneEndogenous Bcl-xLMitochondrial channel activityBcl-xLInhibitor ABT-737ABT-737Outer membraneBcl-xL.Pro-apoptotic cleavage productRecombinant Bcl-xLChannel activityBcl-xL proteinSynaptic functionDual roleGenetic toolsDomain pocketSynaptic transmissionSynaptic activityGiant presynaptic terminalEquivalent modificationEndogenous proteolysisRepetitive synaptic activityBH3Cleavage productsProtein
2004
Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia
Knox RJ, Magoski NS, Wing D, Barbee SJ, Kaczmarek LK. Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia. Developmental Neurobiology 2004, 60: 411-423. PMID: 15307146, DOI: 10.1002/neu.20029.Peer-Reviewed Original ResearchConceptsAplysia bag cell neuronsWhole-cell current-clamp recordingsBag cell neuronsPlasma membraneCurrent-clamp recordingsNeuronal physiologyCytosolic pHCytosolic freeMembrane potentialCell neuronsSodium pyrithionePresence of externalRatiometric imagingMV depolarizationClose structural analogueHill coefficientNapStructural analoguesSpecies
2001
Aplysia Ror Forms Clusters on the Surface of Identified Neuroendocrine Cells
McKay S, Hislop J, Scott D, Bulloch A, Kaczmarek L, Carew T, Sossin W. Aplysia Ror Forms Clusters on the Surface of Identified Neuroendocrine Cells. Molecular And Cellular Neuroscience 2001, 17: 821-841. PMID: 11358481, DOI: 10.1006/mcne.2001.0977.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAmino Acid SequenceAnimalsAntibody SpecificityAplysiaBase SequenceCaenorhabditis elegans ProteinsCell CompartmentationCells, CulturedCloning, MolecularGanglia, InvertebrateImmunohistochemistryMolecular Sequence DataNeuronsNeurosecretory SystemsReceptor Protein-Tyrosine KinasesReceptor Tyrosine Kinase-like Orphan ReceptorsReceptors, Cell SurfaceRNA, MessengerConceptsBag cell neuronsNeuroendocrine bag cell neuronsROR receptorsCultured bag cell neuronsRegulation of growthReceptor tyrosine kinasesMarine mollusk Aplysia californicaPeripheral neuronal processesMollusk Aplysia californicaCellular polarityFunctional domainsTyrosine kinaseIntracellular organellesCell surfaceProteinNeuroendocrine cellsKinaseAplysia californicaRelease sitesNeuronal processesOrganellesNeuronal populationsForm clustersGanglionic neuropilReceptors
1998
Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia
White B, Nick T, Carew T, Kaczmarek L. Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 1998, 80: 2514-2520. PMID: 9819259, DOI: 10.1152/jn.1998.80.5.2514.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalcium ChannelsGanglia, InvertebrateImmunohistochemistryNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesProtein Kinase C
1997
The Secretion of Classical and Peptide Cotransmitters from a Single Presynaptic Neuron Involves a Synaptobrevin-Like Molecule
Whim M, Niemann H, Kaczmarek L. The Secretion of Classical and Peptide Cotransmitters from a Single Presynaptic Neuron Involves a Synaptobrevin-Like Molecule. Journal Of Neuroscience 1997, 17: 2338-2347. PMID: 9065494, PMCID: PMC6573516, DOI: 10.1523/jneurosci.17-07-02338.1997.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsAplysiaCalciumCells, CulturedCoculture TechniquesElectric ConductivityGanglia, InvertebrateKineticsMagnesiumMembrane PotentialsMembrane ProteinsNerve Tissue ProteinsNeuronsNeurons, AfferentNeuropeptidesPatch-Clamp TechniquesPresynaptic TerminalsR-SNARE ProteinsRecombinant ProteinsSynapsesTetanus ToxinConceptsClassical transmittersSingle presynaptic neuronRelease of neuropeptidesSingle action potentialPresynaptic release sitesSecretion of peptidesNeuron B2Peptidergic synapsesSynaptic typesSensory neuronsPresynaptic neuronsTetanus toxinPeptide cotransmittersAction potentialsPresynaptic injectionSecretionNeuronsMolecular mechanismsSynapseTypes of transmittersB2CotransmitterNeuropeptidesPeptidesReleaseIdentification of a Vesicular Pool of Calcium Channels in the Bag Cell Neurons of Aplysia californica
White B, Kaczmarek L. Identification of a Vesicular Pool of Calcium Channels in the Bag Cell Neurons of Aplysia californica. Journal Of Neuroscience 1997, 17: 1582-1595. PMID: 9030618, PMCID: PMC6573390, DOI: 10.1523/jneurosci.17-05-01582.1997.Peer-Reviewed Original ResearchConceptsBag cell neuronsCalcium channel alpha1 subunitAplysia nervous systemProtein kinase CCell neuronsAplysia californicaBag cell clustersCalcium channelsChannel alpha1 subunitCell clustersVesicular channelsMembrane proteinsReverse-transcribed RNAVesicular localizationPlasma membraneEgg-laying hormoneMolecular mechanismsSubcellular distributionKinase CLysoTracker RedDense-core vesiclesAcidic organellesGrowth conesCalcium channel subtypesCalcium current modulation
1996
Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons
Nick T, Kaczmarek L, Carew T. Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons. Journal Of Neuroscience 1996, 16: 7583-7598. PMID: 8922415, PMCID: PMC6579091, DOI: 10.1523/jneurosci.16-23-07583.1996.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCell SeparationCellular SenescenceElectric ConductivityElectrophysiologyGanglia, InvertebrateIonsNeuronsPatch-Clamp TechniquesPotassiumConceptsBag cell neuronsRepetitive firingCell neuronsEgg-laying behaviorJuvenile neuronsTetraethyl ammonium chlorideDevelopmental regulationSpecific ionic currentsAplysia bag cell neuronsAfterdischargesElectrical stimulationVariety of stimuliDevelopmental controlNeuronal systemsProlonged depolarizationBehavioral effectsNeuronsIonic currentsDevelopmental mechanismsSelective regulationAdultsAplysia californicaFiringRegulationDepolarizationIdentification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons
Wilson G, Richardson F, Fisher T, Olivera B, Kaczmarek L. Identification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons. Journal Of Neuroscience 1996, 16: 3661-3671. PMID: 8642410, PMCID: PMC6578840, DOI: 10.1523/jneurosci.16-11-03661.1996.Peer-Reviewed Original ResearchConceptsNonspecific cation channelRepetitive firingCation channelsSpontaneous repetitive firingNeuronal response propertiesBag cell neuronsSlow inwardNeuronal excitabilityCell neuronsChannels underliesProlonged dischargeAplysia bag cell neuronsAplysia neuronsAfterdischargesNeuronsPhosphorylation-mediated changesResponse propertiesChannel recordingsConus textileFiringO mVTetrodotoxinExcitability
1994
Cyclic AMP modulates fast axonal transport in aplysia bag cell neurons by increasing the probability of single organelle movement
Azhderian EM, Hefner D, Lin C, Kaczmarek LK, Forscher P. Cyclic AMP modulates fast axonal transport in aplysia bag cell neurons by increasing the probability of single organelle movement. Neuron 1994, 12: 1223-1233. PMID: 7516686, DOI: 10.1016/0896-6273(94)90439-1.Peer-Reviewed Original ResearchAutoactive peptides act at three distinct receptors to depolarize the bag cell neurons of Aplysia
Loechner K, Kaczmarek L. Autoactive peptides act at three distinct receptors to depolarize the bag cell neurons of Aplysia. Journal Of Neurophysiology 1994, 71: 195-203. PMID: 8158229, DOI: 10.1152/jn.1994.71.1.195.Peer-Reviewed Original Research