2010
Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons
Gazula V, Strumbos JG, Mei X, Chen H, Rahner C, Kaczmarek LK. Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons. The Journal Of Comparative Neurology 2010, 518: 3205-3220. PMID: 20575068, PMCID: PMC2894291, DOI: 10.1002/cne.22393.Peer-Reviewed Original ResearchConceptsPresynaptic terminalsBrainstem auditory neuronsPattern of stainingMNTB neuronsPrincipal neuronsSynaptic markersTrapezoid bodyCochlear nucleusAfferent inputAxonal stainingMedial nucleusAuditory brainstemPresynaptic endingsOlfactory bulbAuditory neuronsPotassium channel genesTonotopic axisTonotopic gradientNeuronsKv1.3 channelsProminent labelingPrincipal cellsAuditory stimuliKv1 familyKv1.3
2004
Kv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics
Fadool DA, Tucker K, Perkins R, Fasciani G, Thompson RN, Parsons AD, Overton JM, Koni PA, Flavell RA, Kaczmarek LK. Kv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics. Neuron 2004, 41: 389-404. PMID: 14766178, PMCID: PMC2737549, DOI: 10.1016/s0896-6273(03)00844-4.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAdaptor Proteins, Vesicular TransportAnimalsBehavior, AnimalBlotting, WesternBody WeightBrain-Derived Neurotrophic FactorCalcium ChannelsCells, CulturedDensitometryDifferential ThresholdDiscrimination, PsychologicalDose-Response Relationship, DrugDrinkingElectric StimulationEmbryo, MammalianEnergy IntakeExploratory BehaviorGene DeletionGRB10 Adaptor ProteinHabituation, PsychophysiologicHumansInsulinKidneyKineticsKv1.3 Potassium ChannelMembrane PotentialsMiceMice, KnockoutMotor ActivityNerve Tissue ProteinsNeuronsNeurotoxinsNuclear Matrix-Associated ProteinsOdorantsOlfactory BulbPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Voltage-GatedProteinsRas ProteinsReceptor, trkBReverse Transcriptase Polymerase Chain ReactionRNA, MessengerScorpion VenomsSensory ThresholdsSrc-Family KinasesTime FactorsTyrosine 3-MonooxygenaseConceptsKv1.3-/- miceProtein-protein interactionsGene-targeted deletionKv1.3-null miceSignal transductionScaffolding proteinSignaling cascadesChannel genesC-type inactivationDeletionMembrane potentialNull miceOlfactory codingDetection of odorsPotassium channelsKv1.3 channelsProteinSense of smellSlow inactivation kineticsWild-type miceTransductionGenesOlfactory bulb mitral cellsMiceRole
2003
The voltage-gated potassium channel Kv1.3 regulates energy homeostasis and body weight
Xu J, Koni PA, Wang P, Li G, Kaczmarek L, Wu Y, Li Y, Flavell RA, Desir GV. The voltage-gated potassium channel Kv1.3 regulates energy homeostasis and body weight. Human Molecular Genetics 2003, 12: 551-559. PMID: 12588802, DOI: 10.1093/hmg/ddg049.Peer-Reviewed Original ResearchConceptsBody weightBasal metabolic rateKv1.3 channelsDiet-induced obesityHigh-fat dietBody weight regulationT cell activationVoltage-gated potassium channel Kv1.3Voltage-gated potassium channelsPotassium channel Kv1.3Control littermatesFood intakeLittermate controlsKnockout miceWeight regulationIndirect calorimetryMetabolic rateChannel inhibitionCell activationEnergy homeostasisKnockout animalsPotassium channelsCell membrane potentialMiceChannel Kv1.3
1996
Inhibition by Nystatin of Kv1.3 Channels Expressed in Chinese Hamster Ovary Cells
HAHN S, WANG L, KACZMAREK L. Inhibition by Nystatin of Kv1.3 Channels Expressed in Chinese Hamster Ovary Cells. Neuropharmacology 1996, 35: 895-901. PMID: 8938720, DOI: 10.1016/0028-3908(96)00094-9.Peer-Reviewed Original ResearchConceptsKv1.3 currentsEffect of nystatinPerforated patch recordingsChinese hamster ovary cellsPatch-clamp techniqueWhole-cell configurationConcentration-dependent reductionHamster ovary cellsRectifier potassium channelHalf-maximal inhibitionPotassium channel Kv1.3Patch recordingsMaximal inhibitionAmphotericin BOvary cellsKv1.3 channelsPotassium channelsTetraethylammoniumChannel Kv1.3Intracellular solutionInhibitionRapid blockNystatinDrugsApparent inactivation