2006
A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia
Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, Magoski NS. A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 2006, 96: 2688-2698. PMID: 16885525, PMCID: PMC2894935, DOI: 10.1152/jn.00118.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnilidesAnimalsAplysiaCalcium Channel BlockersCalcium ChannelsCalcium SignalingElectrophysiologyEnzyme InhibitorsImidazolesIn Vitro TechniquesIndicators and ReagentsIndolesInositol 1,4,5-TrisphosphateLanthanumMacrocyclic CompoundsMembrane PotentialsNeuronsNickelOxazolesRyanodine Receptor Calcium Release ChannelThapsigarginThiadiazolesConceptsBag cell neuronsCell neuronsCyclopiazonic acidCPA-sensitive storesSmooth muscle cell lineAbsence of extracellularCultured bag cell neuronsRyanodine receptor agonistATPase inhibitorReceptor blockersReceptor agonistNeuropeptide secretionXestospongin CMuscle cell lineInflux pathwayIntracellular storesNonneuronal cellsAcidic storesElevated intracellularNeuronsStore depletionAfterdischargesCell linesBafilomycin AEntry pathway
2002
Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons
Zhang Y, Magoski NS, Kaczmarek LK. Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons. Journal Of Neuroscience 2002, 22: 10134-10141. PMID: 12451114, PMCID: PMC6758731, DOI: 10.1523/jneurosci.22-23-10134.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCells, CulturedElectric StimulationEnzyme ActivatorsEnzyme InhibitorsLarge-Conductance Calcium-Activated Potassium ChannelsNeural InhibitionNeuronsPatch-Clamp TechniquesPhloretinPotassiumPotassium Channel BlockersPotassium Channels, Calcium-ActivatedProtein Kinase CRefractory Period, ElectrophysiologicalSphingosineTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsRefractory periodBK currentsProtein kinase COnset of afterdischargeBK channel activityApplication of phloretinBK channel activatorsProlonged refractory periodAbility of stimulationRole of Ca2Blocker paxillinePharmacological characteristicsChannel activatorIntracellular Ca2Prolonged increaseOutward currentsInhibitor of PKCAfterdischargesNeuronsAdditional stimulationProlonged activationActivator of PKCChannel activity
2000
Activation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones
Magoski N, Knox R, Kaczmarek L. Activation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones. The Journal Of Physiology 2000, 522: 271-283. PMID: 10639103, PMCID: PMC2269759, DOI: 10.1111/j.1469-7793.2000.t01-2-00271.x.Peer-Reviewed Original ResearchConceptsVoltage-gated Ca2Non-selective cation channelsRefractory periodCation channelsElectrical stimulationFree salineAbility of nifedipinePresence of nifedipinePresence of TTXIntact abdominal gangliaProlonged refractory periodOnset of refractorinessAfferent inputBrief synaptic stimulationHigh external potassiumPermeable cation channelIntracellular calciumAbdominal ganglionExtracellular Ca2Synaptic stimulationAfterdischargesNormal responseIntracellular Ca2Intracellular storesRepeated stimulation
1998
Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia
Whim M, Kaczmarek L. Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia. Neuroscience Letters 1998, 258: 143-146. PMID: 9885951, DOI: 10.1016/s0304-3940(98)00850-7.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsMetabotropic glutamate receptorsG proteinsPeptidergic bag cell neuronsAfferent stimulationSpontaneous firingAfferent inputGlutamate receptorsSecond messenger pathwaysPharmacological activationReceptor activationAfterdischargesNeuronsMessenger pathwaysReceptorsActivationAdditional pathwaysExpressionExcitabilityPathwaySustained period
1996
Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons
Nick T, Kaczmarek L, Carew T. Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons. Journal Of Neuroscience 1996, 16: 7583-7598. PMID: 8922415, PMCID: PMC6579091, DOI: 10.1523/jneurosci.16-23-07583.1996.Peer-Reviewed Original ResearchConceptsBag cell neuronsRepetitive firingCell neuronsEgg-laying behaviorJuvenile neuronsTetraethyl ammonium chlorideDevelopmental regulationSpecific ionic currentsAplysia bag cell neuronsAfterdischargesElectrical stimulationVariety of stimuliDevelopmental controlNeuronal systemsProlonged depolarizationBehavioral effectsNeuronsIonic currentsDevelopmental mechanismsSelective regulationAdultsAplysia californicaFiringRegulationDepolarizationIdentification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons
Wilson G, Richardson F, Fisher T, Olivera B, Kaczmarek L. Identification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons. Journal Of Neuroscience 1996, 16: 3661-3671. PMID: 8642410, PMCID: PMC6578840, DOI: 10.1523/jneurosci.16-11-03661.1996.Peer-Reviewed Original ResearchConceptsNonspecific cation channelRepetitive firingCation channelsSpontaneous repetitive firingNeuronal response propertiesBag cell neuronsSlow inwardNeuronal excitabilityCell neuronsChannels underliesProlonged dischargeAplysia bag cell neuronsAplysia neuronsAfterdischargesNeuronsPhosphorylation-mediated changesResponse propertiesChannel recordingsConus textileFiringO mVTetrodotoxinExcitability
1994
Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica
Fisher T, Levy S, Kaczmarek L. Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica. Journal Of Neurophysiology 1994, 71: 1254-1257. PMID: 8201416, DOI: 10.1152/jn.1994.71.3.1254.Peer-Reviewed Original ResearchConceptsIntracellular calcium levelsBag cell neuronsCalcium levelsSpontaneous firingCell neuronsIntracellular calciumAction potentialsTransient elevationBasal intracellular calcium levelsLong-duration action potentialsBasal calcium levelsFree calcium ion concentrationTriggers calcium releaseCalcium-sensitive microelectrodesAplysia californicaIndividual action potentialsAfferent stimulationAfferent inputAfterdischargesProlonged increaseCalcium releaseCalcium ion concentrationNeuronsBiphasic patternPrimary cultures
1993
The peptide FMRFa terminates a discharge in Aplysia bag cell neurons by modulating calcium, potassium, and chloride conductances
Fisher T, Lin C, Kaczmarek L. The peptide FMRFa terminates a discharge in Aplysia bag cell neurons by modulating calcium, potassium, and chloride conductances. Journal Of Neurophysiology 1993, 69: 2164-2173. PMID: 7688803, DOI: 10.1152/jn.1993.69.6.2164.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalcium ChannelsCells, CulturedChloride ChannelsElectric StimulationElectrophysiologyFMRFamideGangliaImmunohistochemistryIon ChannelsMembrane PotentialsMembrane ProteinsNeuritesNeuronsNeuropeptidesNeurotransmitter AgentsPotassium ChannelsStereotyped BehaviorTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsAction potentialsElectrical stimulationVoltage-activated calcium currentsOnset of afterdischargePowerful inhibitory influenceIntact abdominal gangliaIon substitution experimentsVoltage-clamp experimentsAfferent nervesProtein kinase C. 5Channel blockersCalcium currentPrimary cell culturesAbdominal ganglionInhibitory influenceAfterdischargesCyclic AMP analogueFMRFaOutward currentsNeuronal processesNeuronsAplysia bag cell neuronsReversal potential
1992
Inhibition of peptide release from invertebrate neurons by the protein kinase inhibitor H-7
Loechner K, Mattessich-Arrandale J, Azhderian E, Kaczmarek L. Inhibition of peptide release from invertebrate neurons by the protein kinase inhibitor H-7. Brain Research 1992, 581: 315-318. PMID: 1393536, DOI: 10.1016/0006-8993(92)90724-n.Peer-Reviewed Original ResearchHyperosmotic media inhibit voltage-dependent calcium influx and peptide release in Aplysia neurons
Loechner K, Knox R, Connor J, Kaczmarek L. Hyperosmotic media inhibit voltage-dependent calcium influx and peptide release in Aplysia neurons. The Journal Of Membrane Biology 1992, 128: 41-52. PMID: 1323684, DOI: 10.1007/bf00231869.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsAction potentialsVoltage-dependent calcium influxVoltage-dependent calcium currentsPotassium-induced depolarizationIntracellular calcium levelsCalcium indicator dyeEffect of hyperosmolalityHyperosmotic mediumCalcium currentCalcium influxFura-2Calcium levelsPeptide secretionCalcium entryAfterdischargesBrief stimulationNeuroactive peptidesNeuronsSecretory propertiesAplysia neuronsHigh potassiumPeptide releaseRelease of peptides
1990
Control of potassium currents and cyclic AMP levels by autoactive neuropeptides in Aplysia neurons
Loechner K, Kaczmarek L. Control of potassium currents and cyclic AMP levels by autoactive neuropeptides in Aplysia neurons. Brain Research 1990, 532: 1-6. PMID: 2178030, DOI: 10.1016/0006-8993(90)91733-w.Peer-Reviewed Original ResearchConceptsVoltage-dependent potassium currentsCyclic AMP levelsBag cell neuronsPotassium currentCell neuronsAMP levelsAlpha-BCPWhole-cell patch-clamp techniqueCell patch-clamp techniqueBag cell afterdischargeTransient voltage-dependent potassium currentPatch-clamp techniqueDelayed potassium currentPharmacologic elevationClamp techniqueOutward currentsAfterdischargesNeuronsCell peptidesAplysia neuronsNeuropeptidesBeta-BCPConsistent effectSecond messengerAlphaCyclic AMP regulates processing of neuropeptide precursor in bag cell neurons ofAplysia
Azhderian E, Kaczmarek L. Cyclic AMP regulates processing of neuropeptide precursor in bag cell neurons ofAplysia. Journal Of Molecular Neuroscience 1990, 2: 61-70. PMID: 1964064, DOI: 10.1007/bf02876912.Peer-Reviewed Original ResearchConceptsBag cell neuronsEgg-laying hormoneCell neuronsCAMP levelsPresence of tetrodotoxinBag cell clustersAdenylate cyclase activator forskolinELH prohormoneCyclase activator forskolinElevation of cAMPMembrane-permeant cAMP analogAbdominal ganglionControl clustersPermeant cAMP analogueAction potentialsNeuroactive peptidesTreatment of cellsProtein synthesis inhibitor anisomycinActivator forskolinNeuronsProhormoneAfterdischargesCyclic AMPTetrodotoxinComparative peptide mapping
1986
Multiple components of delayed potassium current in peptidergic neurons of Aplysia: modulation by an activator of adenylate cyclase
Strong J, Kaczmarek L. Multiple components of delayed potassium current in peptidergic neurons of Aplysia: modulation by an activator of adenylate cyclase. Journal Of Neuroscience 1986, 6: 814-822. PMID: 2420948, PMCID: PMC6568473, DOI: 10.1523/jneurosci.06-03-00814.1986.Peer-Reviewed Original ResearchConceptsAction potentialsApplication of forskolinBag cell neuronsPotassium currentCell neuronsOutward currentsWhole-cell patch-clamp techniqueBag cell afterdischargePatch-clamp techniqueEffect of forskolinElevation of cAMPPeptidergic neuronsCalcium entryClamp techniqueAfterdischargesReproductive tractMsec depolarizationsAdenylate cyclasePrimary culturesHigh intracellular concentrationsNeuronsMin periodRepetitive activitySpike widthForskolin
1985
Inhibitors of calcium-dependent enzymes prevent the onset of afterdischarge in the peptidergic bag cell neurons of Aplysia
DeRiemer S, Schweitzer B, Kaczmarek L. Inhibitors of calcium-dependent enzymes prevent the onset of afterdischarge in the peptidergic bag cell neurons of Aplysia. Brain Research 1985, 340: 175-180. PMID: 4027644, DOI: 10.1016/0006-8993(85)90790-5.Peer-Reviewed Original ResearchConceptsBag cell neuronsCalcium-dependent enzymesCell neuronsNeuroactive peptidesAttenuation of secretionOnset of afterdischargeBrief electrical stimulationPeptidergic bag cell neuronsAbility of stimulationEffect of TFPEffects of agentsNeuronal excitabilitySynaptic transmissionAfterdischargesNeurosecretory neuronsElectrical stimulationEndogenous burstingNeuronsSecretionCalmodulin-dependent enzymesProtein kinaseAplysiaStimulationOnsetAgents
1983
Calcium entry causes a prolonged refractory period in peptidergic neurons of Aplysia
Kaczmarek L, Kauer J. Calcium entry causes a prolonged refractory period in peptidergic neurons of Aplysia. Journal Of Neuroscience 1983, 3: 2230-2239. PMID: 6631477, PMCID: PMC6564640, DOI: 10.1523/jneurosci.03-11-02230.1983.Peer-Reviewed Original ResearchConceptsOnset of afterdischargeProlonged refractory periodBag cell clustersBag cell neuronsCumulative depolarizationRefractory periodNatural refractorinessCell neuronsCalcium entryAction potentialsConcentration of ionophoreExtracellular tetraethylammonium ionsRepetitive intracellular stimulationPeptidergic bag cell neuronsPleuroabdominal connectivesCell clustersCalcium-deficient mediumAdenylate cyclase activatorCalcium-containing mediumMean durationPeptidergic neuronsAbdominal ganglionAfterdischargesBrief trainsIntracellular stimulation
1981
Serotonergic inhibition of afterdischarge in peptidergic bag cells
Jennings K, Host J, Kaczmarek L, Strumwasser F. Serotonergic inhibition of afterdischarge in peptidergic bag cells. Developmental Neurobiology 1981, 12: 579-590. PMID: 7310396, DOI: 10.1002/neu.480120606.Peer-Reviewed Original ResearchConceptsEgg-laying hormoneAfterdischarge activityConnective nervesBag cellsSpike frequencyPotassium channel blocker tetraethylammoniumEffects of serotoninBag cell afterdischargeChannel blocker tetraethylammoniumDose-dependent mannerResult of serotoninPeptide hormone releaseNeuroendocrine bag cellsSerotonergic inhibitionSerotonin inhibitionSerotonergic synapsesSerotonergic inputBag cell neuronsHormone releaseIntracellular recordingsSerotonin receptorsBlocker tetraethylammoniumD-butaclamolAfterdischargesElectrical stimulation
1979
The morphology and coupling of Aplysia bag cells within the abdominal ganglion and in cell culture
Kaczmarek L, Finbow M, Revel J, Strumwasser F. The morphology and coupling of Aplysia bag cells within the abdominal ganglion and in cell culture. Developmental Neurobiology 1979, 10: 535-550. PMID: 521813, DOI: 10.1002/neu.480100604.Peer-Reviewed Original ResearchConceptsBag cell clustersBag cellsAbdominal ganglionLucifer YellowConnective tissueBag cell afterdischargeCell clustersSynchronous action potentialsIntracellular injectionIntact gangliaAction potentialsGangliaPrimary culturesNeurosecretory cellsDistal processesInjected cellsAfterdischargesCell actionGap junctionsSomaDye transferCellsPairs of cellsCell culturesCell processes