Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles
Li Y, Monte A, Dylla L, Moghimi S, Simberg D. Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles. Journal Of Immunological Methods 2024, 528: 113668. PMID: 38574804, PMCID: PMC11023749, DOI: 10.1016/j.jim.2024.113668.Peer-Reviewed Original ResearchConceptsDeposition of C3Complement activation markersSurface C3 depositionMembrane attack complex C5b-9Activation markersDot blot immunoassayC3 depositionAnaphylatoxins C3aImmune responseNanomedicine researchC5b-9Complement attackComplement activationBlot immunoassayFluid phase markerMarkersPhase markersImmunoassaySurface resultsPathway specificityAssembly of C3 convertaseComplementNanoparticles