2016
P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches
Rewinski M, Ortiz J, Alberghini T, Bow L, Devivo J, Doyle P, Michalski T. P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches. Human Immunology 2016, 77: 78. DOI: 10.1016/j.humimm.2016.07.119.Peer-Reviewed Original ResearchT cell populationsPatient seraMedian fluorescence intensityT cellsComplement proteinsControl seraUntreated patients' seraFlow cytometric crossmatchDonor cellsSignificant T-cellCell populationsOccasional false positive resultsHigh-titer antibodiesPatient serum samplesCytometric crossmatchFlow crossmatchAntibody testingFalse-positive resultsIgM antibodiesPositive control seraMFI levelsNegative control seraTiter antibodiesSerum samplesSerum
2015
Conundrum: A negative crossmatch in a patient with high level donor specific antibody
Rewinski M, Alberghini T, Bow L, Devivo J, Doyle P, Michalski P, Ortiz-Rivera J. Conundrum: A negative crossmatch in a patient with high level donor specific antibody. Human Immunology 2015, 76: 57. DOI: 10.1016/j.humimm.2015.07.082.Peer-Reviewed Original ResearchDonor-specific antibodiesB-cell crossmatchDonor-recipient pairsNegative crossmatchMedian fluorescence intensityVirtual crossmatchPresence of DSAPositive B cell crossmatchSpecific antibodiesIncompatible donor-recipient pairsFlow crossmatchAntibody levelsPositive donorsSAB assayAntibody testingHigh-resolution typingClinical significanceT cellsHLA typingPatient seraCrossmatchEP serumClass IIDQ7RT-PCR