1988
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries
Lichter P, Cremer T, Borden J, Manuelidis L, Ward D. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988, 80: 224-234. PMID: 3192212, DOI: 10.1007/bf01790090.Peer-Reviewed Original ResearchConceptsIndividual human chromosomesHuman chromosomesDNA insertsInterphase nucleiInterphase cellsTotal human genomic DNASitu suppression hybridizationPost-hybridization washesRecombinant DNA libraryHuman genomic DNAChromosomal domainsConfocal fluorescence microscopyChromosomal librarySuppression hybridizationDNA librarySingle-strand DNARepetitive sequencesCognate chromosomeChromosome 1Genomic DNAChromosomesRecombinant libraryKaryotypic studiesMetaphase spreadsChromosomal preparations
1985
Individual interphase chromosome domains revealed by in situ hybridization
Manuelidis L. Individual interphase chromosome domains revealed by in situ hybridization. Human Genetics 1985, 71: 288-293. PMID: 3908288, DOI: 10.1007/bf00388453.Peer-Reviewed Original ResearchConceptsInterphase chromosomesHuman chromosomesMouse-human cell hybridsCharacteristic folding patternSingle human chromosomeHuman interphase chromosomesSitu hybridizationHuman DNA probesInterphase chromosome domainsChromosome domainsIndividual chromosomesCell hybridsInterphase nucleiChromosomesDNA probesHybrid cellsFolding patternInterphase domainHybridizationOptical sectionsDomainNucleusWide fibersHybridization solutionReproducible position