Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries
Lichter P, Cremer T, Borden J, Manuelidis L, Ward D. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988, 80: 224-234. PMID: 3192212, DOI: 10.1007/bf01790090.Peer-Reviewed Original ResearchConceptsIndividual human chromosomesHuman chromosomesDNA insertsInterphase nucleiInterphase cellsTotal human genomic DNASitu suppression hybridizationPost-hybridization washesRecombinant DNA libraryHuman genomic DNAChromosomal domainsConfocal fluorescence microscopyChromosomal librarySuppression hybridizationDNA librarySingle-strand DNARepetitive sequencesCognate chromosomeChromosome 1Genomic DNAChromosomesRecombinant libraryKaryotypic studiesMetaphase spreadsChromosomal preparations