2022
Anatomic nomenclature and 3-dimensional regional model of the human ovary: call for a new paradigm
O'Neill KE, Maher JY, Laronda MM, Duncan FE, LeDuc RD, Lujan ME, Oktay KH, Pouch AM, Segars JH, Tsui EL, Zelinski MB, Halvorson LM, Gomez-Lobo V. Anatomic nomenclature and 3-dimensional regional model of the human ovary: call for a new paradigm. American Journal Of Obstetrics And Gynecology 2022, 228: 270-275.e4. PMID: 36191605, PMCID: PMC9974561, DOI: 10.1016/j.ajog.2022.09.040.Peer-Reviewed Original Research
2021
Individual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation
Titus S, Szymanska K, Musul B, Turan V, Taylan E, Garcia- Milian R, Mehta S, Oktay K. Individual-oocyte transcriptomic analysis shows that genotoxic chemotherapy depletes human primordial follicle reserve in vivo by triggering proapoptotic pathways without growth activation. Scientific Reports 2021, 11: 407. PMID: 33431979, PMCID: PMC7801500, DOI: 10.1038/s41598-020-79643-x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntineoplastic Combined Chemotherapy ProtocolsApoptosisCyclophosphamideDNA DamageFemaleGene Expression ProfilingHeterograftsHumansMiceMice, Inbred NODMice, SCIDOocytesOogenesisOvarian FollicleOvarian ReserveOvarySignal TransductionSingle-Cell AnalysisTranscriptomeYoung AdultConceptsPrimordial follicle oocytesFollicle lossGrowth activationHuman ovarian xenograft modelPI3K/PTEN/AktFollicle oocytesDNA damagePI3K/PTEN/Akt pathwayOvarian reserve depletionPrimordial follicle reservePTEN/AKT pathwayIngenuity Pathway AnalysisOvarian xenograft modelPTEN/AKTSevere DNA damageExpression of AktGonadotoxic chemotherapyAnti-apoptotic Bcl2Early menopauseFollicle reserveTranscriptomic analysisCyclophosphamide injectionHuman ovaryPhosphorylation statusRNA sequencing
2020
Unraveling the mechanisms of chemotherapy-induced damage to human primordial follicle reserve: road to developing therapeutics for fertility preservation and reversing ovarian aging
Szymanska K, Tan X, Oktay K. Unraveling the mechanisms of chemotherapy-induced damage to human primordial follicle reserve: road to developing therapeutics for fertility preservation and reversing ovarian aging. Molecular Human Reproduction 2020, 26: 553-566. PMID: 32514568, PMCID: PMC7411370, DOI: 10.1093/molehr/gaaa043.Peer-Reviewed Original ResearchConceptsPrimordial follicle reserveChemotherapy-induced damageFollicle reserveOvarian reserveChemotherapy-induced lossOvarian reserve depletionApoptotic deathPrimordial follicle oocytesFertility preservationAcute reductionOvarian agingHuman ovaryFollicle activationPrimordial folliclesAcute exposureStromal damageTranslational evidenceChemotherapeutic agentsApoptotic lossFollicle oocytesDNA double-strand breaksDNA DSBsCurrent literatureDeathActivation
2019
Chemotherapy causes primordial follicle death in the human ovary via multiple apoptotic pathways and not by “burn out”
Titus S, Oktay K. Chemotherapy causes primordial follicle death in the human ovary via multiple apoptotic pathways and not by “burn out”. Fertility And Sterility 2019, 112: e84. DOI: 10.1016/j.fertnstert.2019.07.340.Peer-Reviewed Original Research
2007
A novel xenograft model to test the ovarian toxicity of chemotherapy agents in women
Oktay K, Oktem O. A novel xenograft model to test the ovarian toxicity of chemotherapy agents in women. Journal Of Clinical Oncology 2007, 25: 19504-19504. DOI: 10.1200/jco.2007.25.18_suppl.19504.Peer-Reviewed Original ResearchXenograft modelFollicle lossClinical trialsChemotherapy agentsHuman ovarian xenograft modelPrimordial follicle countsNovel xenograft modelOvarian xenograft modelOvarian reserveFollicle countMice 12Ovarian toxicityCY injectionGonadotoxic effectsPremenopausal femalesSingle doseHuman ovaryOvarian piecesClinical studiesImmunodeficient miceSingle injectionNew agentsOvarian histologyXenograft vasculatureTUNEL staining
1998
Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone.
Oktay K, Newton H, Mullan J, Gosden R. Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Human Reproduction 1998, 13: 1133-1138. PMID: 9647533, DOI: 10.1093/humrep/13.5.1133.Peer-Reviewed Original ResearchConceptsWeeks of graftingSevere combined immunodeficiencyFollicle growthOvarian xenograftsAntral stageOestradiol levelsLong-term graftHuman ovarian xenograftsHuman follicle developmentHuman ovarian tissueOvarian follicular growthPreantral follicle growthLong-term graftingGranulosa cell layerHuman primordial folliclesCell nuclear antigenFSH administrationHpg miceKidney capsuleGonadotrophin stimulationRenal capsuleHuman ovaryImmunodeficient miceFollicular growthSmall follicles
1997
Ontogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles1
Oktay K, Briggs D, Gosden R. Ontogeny of Follicle-Stimulating Hormone Receptor Gene Expression in Isolated Human Ovarian Follicles1. The Journal Of Clinical Endocrinology & Metabolism 1997, 82: 3748-3751. PMID: 9360535, DOI: 10.1210/jcem.82.11.4346.Peer-Reviewed Original ResearchConceptsMessenger ribonucleic acidPrimordial folliclesFollicle stageElective cesarean sectionHuman ovarian folliclesPreantral follicle developmentFSH receptor geneHormone receptor gene expressionReceptor gene expressionFollicle-stimulating hormone receptor gene expressionCesarean sectionRectus muscleHuman ovaryAntral folliclesFollicular growthFSH expressionHuman folliculogenesisOvarian folliclesOvarian tissueGranulosa cellsMultilaminar folliclesStromal tissueSingle follicleFollicle developmentFollicles