2024
Somatic mosaicism in schizophrenia brains reveals prenatal mutational processes
Maury E, Jones A, Seplyarskiy V, Nguyen T, Rosenbluh C, Bae T, Wang Y, Abyzov A, Khoshkhoo S, Chahine Y, Zhao S, Venkatesh S, Root E, Voloudakis G, Roussos P, Network B, Park P, Akbarian S, Brennand K, Reilly S, Lee E, Sunyaev S, Walsh C, Chess A. Somatic mosaicism in schizophrenia brains reveals prenatal mutational processes. Science 2024, 386: 217-224. PMID: 39388546, PMCID: PMC11490355, DOI: 10.1126/science.adq1456.Peer-Reviewed Original ResearchConceptsTranscription factor binding sitesWhole-genome sequencingOpen chromatinMutational processesSomatic mutationsFactor binding sitesSchizophrenia casesSchizophrenia risk genesSomatic mosaicismSomatic variantsRisk genesG mutationGene expressionGermline mutationsBinding sitesGenesMutationsIncreased somatic mutationsChromatinMosaic somatic mutationsPrenatal neurogenesisContext of schizophreniaBrain neuronsSchizophrenia brainVariants
2017
Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis
Julia T, Carvalho C, Yuan B, Gu S, Altheimer A, McCarthy S, Malhotra D, Sebat J, Siegel A, Rudolph U, Lupski J, Levy D, Brennand K. Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis. Stem Cell Reports 2017, 8: 519-528. PMID: 28216146, PMCID: PMC5355568, DOI: 10.1016/j.stemcr.2017.01.010.Peer-Reviewed Original Research
2015
Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders
Brennand KJ, Marchetto MC, Benvenisty N, Brüstle O, Ebert A, Belmonte J, Kaykas A, Lancaster MA, Livesey FJ, McConnell MJ, McKay RD, Morrow EM, Muotri AR, Panchision DM, Rubin LL, Sawa A, Soldner F, Song H, Studer L, Temple S, Vaccarino FM, Wu J, Vanderhaeghen P, Gage FH, Jaenisch R. Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders. Stem Cell Reports 2015, 5: 933-945. PMID: 26610635, PMCID: PMC4881284, DOI: 10.1016/j.stemcr.2015.10.011.Peer-Reviewed Original Research
2013
Mosaic Copy Number Variation in Human Neurons
McConnell MJ, Lindberg MR, Brennand KJ, Piper JC, Voet T, Cowing-Zitron C, Shumilina S, Lasken RS, Vermeesch JR, Hall IM, Gage FH. Mosaic Copy Number Variation in Human Neurons. Science 2013, 342: 632-637. PMID: 24179226, PMCID: PMC3975283, DOI: 10.1126/science.1243472.Peer-Reviewed Original ResearchConceptsCopy number variationsHiPSC-derived neuronsSingle-cell genomic approachesNumber variationsDNA copy number variationsSingle-cell sequencingHuman neuronsLarge copy number variationsStem cell linesNeural progenitor cellsNovo copy-number variationsPluripotent stem cell lineAneuploid neuronsGenomic approachesDe novo copy-number variationsSubchromosomal copy number variationsAberrant genomesFrontal cortex neuronsLarge deletionsProgenitor cellsCell linesSubset of neuronsEuploid neuronsDeletionMultiple alterations
2007
All β Cells Contribute Equally to Islet Growth and Maintenance
Brennand K, Huangfu D, Melton D. All β Cells Contribute Equally to Islet Growth and Maintenance. PLOS Biology 2007, 5: e163. PMID: 17535113, PMCID: PMC1877817, DOI: 10.1371/journal.pbio.0050163.Peer-Reviewed Original ResearchMeSH KeywordsAdult Stem CellsAnimalsCell DifferentiationCell ProliferationFemaleGenes, ReporterGenetic MarkersGreen Fluorescent ProteinsHistonesIn Vitro TechniquesInsulin-Secreting CellsIslets of LangerhansMaleMiceMice, Inbred C57BLMice, TransgenicModels, BiologicalMosaicismRecombinant Fusion ProteinsTetracyclineConceptsBeta-cell populationBeta cellsBeta-cell replicationHealthy adult miceBeta-cell poolCell populationsDifferentiated beta cellsStem cellsReplacement therapyCell replacement therapyAdult miceIslet growthΒ-cellsProtein expressionCell poolReplicative capacityCell replicationHepatocyte populationAdult stem cellsClonal analysisCellsLevel of fluorescenceFluorescent protein expressionPopulationDiabetes