2021
Applying stem cells and CRISPR engineering to uncover the etiology of schizophrenia
Michael Deans P, Brennand K. Applying stem cells and CRISPR engineering to uncover the etiology of schizophrenia. Current Opinion In Neurobiology 2021, 69: 193-201. PMID: 34010781, PMCID: PMC8387340, DOI: 10.1016/j.conb.2021.04.003.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCell type-specific fashionStem cell biologyType-specific fashionDisease-associated variantsNeural cell typesCommon genetic variantsMore genesCell biologyCRISPR engineeringGene manipulationGene targetsCRISPR technologyMolecular geneticsInvaluable advancesCell typesHiPSC technologyGenetic variantsStem cellsIndividual variantsEtiology of diseasePolygenic disorderVariantsComplex interactionsRecent advancesEtiology of schizophrenia
2019
Examining the relationship between astrocyte dysfunction and neurodegeneration in ALS using hiPSCs
Halpern M, Brennand KJ, Gregory J. Examining the relationship between astrocyte dysfunction and neurodegeneration in ALS using hiPSCs. Neurobiology Of Disease 2019, 132: 104562. PMID: 31381978, PMCID: PMC6834907, DOI: 10.1016/j.nbd.2019.104562.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisAstrocyte dysfunctionNeurodegenerative diseasesRole of astrocytesNon-cell autonomous mechanismsFatal neurodegenerative diseaseRisk-associated genesAstrocytic dysfunctionNeural cell typesAstrocyte functionDisease onsetDisease progressionMotor neuronsLateral sclerosisTherapeutic interventionsDysfunctionDisease initiationGenetic factorsPotential targetProgressionAutonomous mechanismsDiseaseStem cellsNeurodegenerationCell types
2017
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes
Ho S, Hartley B, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani H, Akbarian S, Brennand K. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports 2017, 9: 615-628. PMID: 28757163, PMCID: PMC5550013, DOI: 10.1016/j.stemcr.2017.06.012.Peer-Reviewed Original ResearchConceptsSynthetic activationRisk genesCell typesModulation of transcriptionNeuropsychiatric risk genesCommon single nucleotide variantsCas9 fusion proteinsEndogenous expression levelsNeural cell typesPluripotent stem cell-derived neural progenitor cellsRare copy number variationsCopy number variationsSingle nucleotide variantsNeural progenitor cellsGene functionFunctional annotationGenetic studiesGenesRisk variantsProgenitor cellsExpression levelsTranscriptionRepressionPositional effectsProtein
2014
Evolving toward a human-cell based and multiscale approach to drug discovery for CNS disorders
Schadt E, Buchanan S, Brennand K, Merchant K. Evolving toward a human-cell based and multiscale approach to drug discovery for CNS disorders. Frontiers In Pharmacology 2014, 5: 252. PMID: 25520658, PMCID: PMC4251289, DOI: 10.3389/fphar.2014.00252.Peer-Reviewed Original ResearchBiology approachNeurological disease pathwaysSpecific neural cell typesSystems biology approachPatient-derived hiPSCsDrug discoveryGeneration of hiPSCsInduced pluripotent stem cellsRepresentative neurological diseasesHuman induced pluripotent stem cellsNeural cell typesPluripotent stem cellsSingle geneGlobal epigeneticExpression studiesFrequent clinical failuresDrug screening strategiesNeurodegenerative diseases Alzheimer's diseaseHuman diseasesPsychiatric disorders schizophreniaCell typesCNS disordersHiPSC linesDisease pathwaysBiological networks
2010
High-Efficient Generation of Induced Pluripotent Stem Cells from Human Astrocytes
Ruiz S, Brennand K, Panopoulos A, Herrerías A, Gage F, Izpisua-Belmonte J. High-Efficient Generation of Induced Pluripotent Stem Cells from Human Astrocytes. PLOS ONE 2010, 5: e15526. PMID: 21170306, PMCID: PMC3000364, DOI: 10.1371/journal.pone.0015526.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsHuman somatic cell typesEmbryoid body generationHuman embryonic stem cellsEmbryonic germ layersEmbryonic stem cellsStem cellsInduced pluripotent stem cellsTeratoma formationRegenerative medicineSomatic cell typesPluripotent markersReprogramming efficiencyHuman somatic cellsHiPS cellsHigh-efficient generationGerm layersAutologous cellsEfficiencySomatic cellsSimilar efficiencyPluripotencyNeural cell typesCell typesLayer