2009
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Gulcicek E, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 2009, 905-917. DOI: 10.1007/978-1-59745-198-7_99.Peer-Reviewed Original ResearchProtein of interestSDS-polyacrylamide gelsMass spectrometryGel digestion procedureChemical sequencingLaser desorption ionization mass spectrometryDesorption ionization mass spectrometryElectrospray ionization sourceIonization mass spectrometryComplex biological mixturesMass spectral acquisitionProtein identification analysisTandem mass spectrometrySDS-PAGEMass spectrometric analysisPolyacrylamide gelsIonization sourceNumerous genomesBiological mixturesPeptide mass dataProtein databaseCleavage procedureMass spectrometerGel matrixLC system
2002
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. 2002, 0: 511-521. DOI: 10.1385/1-59259-169-8:511.Peer-Reviewed Original ResearchPolyacrylamide gel matrixFree NH2 terminusPeptide fractionationCleavage procedureGel matrixFinal purificationChemical cleavagePolyacrylamide gelsEdman degradationMost eukaryotic proteinsSDS-polyacrylamide gelsPVDFGelSpecific proteasesPurificationSDS-PAGECleavageNH2 terminusFractionationPartial amino acid sequenceDegradation
1996
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 1996, 415-425. DOI: 10.1007/978-1-60327-259-9_71.Peer-Reviewed Original ResearchGel digestion procedurePolyacrylamide gel matrixPolyacrylamide gelsFree NH2 terminusPeptide fractionationCleavage procedurePonceau SGel matrixFinal purificationReversed-phase HPLCSDS-polyacrylamide gelsChemical cleavageDigestion procedureCoomassie blueProteolytic digestionSitu digestionEnzymatic digestionGelEdman degradationNitrocellulose membraneMost eukaryotic proteinsSDS-PAGEPVDFSolutionDigestion
1995
Digestion of Proteins in Gels for Sequence Analysis
Stone K, Williams K. Digestion of Proteins in Gels for Sequence Analysis. Current Protocols In Protein Science 1995, 00: 11.3.1-11.3.13. DOI: 10.1002/0471140864.ps1103s00.Peer-Reviewed Original ResearchProtein samplesSDS-PAGEDigestion of proteinsSDS-polyacrylamide gelsAbsence of detergentCDNA cloningAmino terminusPartial sequencesSequencing studiesSequence analysisInternal sequencingInhibits trypsinAmino acid analysisAmount of proteinProteinAlternate protocolGel slicesAcid analysisEnzymatic cleavageReversed-phase HPLCSubsequent washing stepsResidual SDSPmol amountsCloningTerminus
1989
ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine- innervated brain regions. I. Amino acid sequence of ARPP-21B from bovine caudate nucleus
Williams K, Hemmings H, LoPresti M, Greengard P. ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine- innervated brain regions. I. Amino acid sequence of ARPP-21B from bovine caudate nucleus. Journal Of Neuroscience 1989, 9: 3631-3637. PMID: 2552036, PMCID: PMC6569913, DOI: 10.1523/jneurosci.09-10-03631.1989.Peer-Reviewed Original ResearchConceptsARPP-21CAMP-dependent protein kinaseMolecular massMajor cytosolic substrateDopamine-innervated brain regionsAmino acid sequenceAmino acid sequencingProtein phosphorylationCytosolic substratesProtein kinaseAcid sequenceSeryl residuesHistidinyl residuesMolecular mechanismsBovine caudate nucleusPrimary structureNH2-terminalEdman degradationDopamine-innervated regionsPolypeptide chainAmino acid analysisCysteinyl residuesGas-phase sequencingPosition 55SDS-PAGE