2002
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. 2002, 0: 511-521. DOI: 10.1385/1-59259-169-8:511.Peer-Reviewed Original ResearchPolyacrylamide gel matrixFree NH2 terminusPeptide fractionationCleavage procedureGel matrixFinal purificationChemical cleavagePolyacrylamide gelsEdman degradationMost eukaryotic proteinsSDS-polyacrylamide gelsPVDFGelSpecific proteasesPurificationSDS-PAGECleavageNH2 terminusFractionationPartial amino acid sequenceDegradation
1997
Enzymatic cleavage and HPLC peptide mapping of proteins
Williams K, Stone K. Enzymatic cleavage and HPLC peptide mapping of proteins. Molecular Biotechnology 1997, 8: 155-167. PMID: 9406186, DOI: 10.1007/bf02752260.Peer-Reviewed Original ResearchConceptsProteolytic digestsMapping of proteinsReversephase HPLCFinal purificationSodium dodecylEnzymatic cleavageCoomassie blue
1996
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 1996, 415-425. DOI: 10.1007/978-1-60327-259-9_71.Peer-Reviewed Original ResearchGel digestion procedurePolyacrylamide gel matrixPolyacrylamide gelsFree NH2 terminusPeptide fractionationCleavage procedurePonceau SGel matrixFinal purificationReversed-phase HPLCSDS-polyacrylamide gelsChemical cleavageDigestion procedureCoomassie blueProteolytic digestionSitu digestionEnzymatic digestionGelEdman degradationNitrocellulose membraneMost eukaryotic proteinsSDS-PAGEPVDFSolutionDigestion