2002
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. 2002, 0: 533-540. DOI: 10.1385/1-59259-169-8:533.Peer-Reviewed Original ResearchReversed-phase HPLCEnzymatic digestsLaser desorption mass spectrometrySites of chemicalMatrix-assisted laser desorption mass spectrometryRelative elution positionsDesorption mass spectrometryMobile phase resultsReversed-phase HPLC separationTotal hydrophobicityAqueous mixturesMass spectrometryVolatile solventsReversephase HPLCHPLC separationLarge peptidesParticular separationComplex mixturesExcellent reproducibilitySlow kineticsEdman sequencingRetention coefficientTight bindingHydrophobic amino acidsHPLC
1996
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. Springer Protocols Handbooks 1996, 427-434. DOI: 10.1007/978-1-60327-259-9_72.Peer-Reviewed Original ResearchReversed-phase HPLCEnzymatic digestsLaser desorption mass spectrometrySites of chemicalMatrix-assisted laser desorption mass spectrometryRelative elution positionsDesorption mass spectrometryMobile phase resultsReversed-phase HPLC separationTotal hydrophobicityAqueous mixturesMass spectrometryVolatile solventsHPLC separationLarge peptidesComplex mixturesParticular separationExcellent reproducibilitySlow kineticsEdman sequencingRetention coefficientTight bindingHydrophobic amino acidsHPLCRelative insolubility
1990
[21] Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins
Stone K, Elliott J, Peterson G, McMurray W, Williams K. [21] Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins. Methods In Enzymology 1990, 193: 389-412. PMID: 2074828, DOI: 10.1016/0076-6879(90)93429-o.Peer-Reviewed Original ResearchConceptsHigh-performance liquid chromatographyReversed-phase high-performance liquid chromatographyReversed phase high performance liquid chromatographyLiquid chromatographyEnzymatic digestsHigh peak capacityMass spectrometric approachProtein chemistsSpectrometric approachMass spectrometryPeak capacityComplex mixturesMolecular weightChemical cleavageGradient timeCleavage productsChromatographyTryptic peptidesPeptidesDigestsChemistsSpectrometryFractionationProductsPrimary structure