1996
Purification and characterization of a recombinant hepatitis E protein vaccine candidate by liquid chromatography-mass spectrometry
McAtee C, Zhang Y, Yarbough P, Fuerst T, Stone K, Samander S, Williams K. Purification and characterization of a recombinant hepatitis E protein vaccine candidate by liquid chromatography-mass spectrometry. Journal Of Chromatography B 1996, 685: 91-104. PMID: 8930757, DOI: 10.1016/0378-4347(96)00143-0.Peer-Reviewed Original ResearchConceptsMass spectrometryCarboxyl terminusReversed phase liquid chromatographyAmino terminusLaser desorption mass spectrometryDesorption mass spectrometryMolecular massLiquid chromatography-mass spectrometryCarboxyl-terminal sequencingChromatography-mass spectrometryBaculovirus expression vectorSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisLC-MSLiquid chromatographyExpression vectorTerminal sequencingSequence analysisPolyacrylamide gel electrophoresisSpectrometryIntact proteinInternal sequencesDoublet proteinsTerminus
1995
Digestion of Proteins in Gels for Sequence Analysis
Stone K, Williams K. Digestion of Proteins in Gels for Sequence Analysis. Current Protocols In Protein Science 1995, 00: 11.3.1-11.3.13. DOI: 10.1002/0471140864.ps1103s00.Peer-Reviewed Original ResearchProtein samplesSDS-PAGEDigestion of proteinsSDS-polyacrylamide gelsAbsence of detergentCDNA cloningAmino terminusPartial sequencesSequencing studiesSequence analysisInternal sequencingInhibits trypsinAmino acid analysisAmount of proteinProteinAlternate protocolGel slicesAcid analysisEnzymatic cleavageReversed-phase HPLCSubsequent washing stepsResidual SDSPmol amountsCloningTerminus
1985
Identification of a nucleic acid helix-destabilizing protein from rat liver as lactate dehydrogenase-5.
Williams K, Reddigari S, Patel G. Identification of a nucleic acid helix-destabilizing protein from rat liver as lactate dehydrogenase-5. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 5260-5264. PMID: 2991914, PMCID: PMC390547, DOI: 10.1073/pnas.82.16.5260.Peer-Reviewed Original ResearchConceptsHelix-destabilizing proteinSs-DNAAmino acid compositionHPLC tryptic peptide mapsNucleic acid helix-destabilizing proteinSolid-phase protein sequencingChemical modification studiesCoenzyme binding siteTyrosine-238Molecular weightSimilar amino acid compositionsTryptic peptide mapsAcid compositionLactate dehydrogenase 5Molecular homogeneitySimilar specific activitiesProtein sequencingLDH proteinDNA bindingAmino terminusBiological roleSingle proteinM chainTryptic peptidesVivo role