1988
In vitro adenylate cyclase-stimulating activity predicts the occurrence of humoral hypercalcemia of malignancy in nude mice.
Weir EC, Insogna KL, Brownstein DG, Bander NH, Broadus AE. In vitro adenylate cyclase-stimulating activity predicts the occurrence of humoral hypercalcemia of malignancy in nude mice. Journal Of Clinical Investigation 1988, 81: 818-821. PMID: 3343341, PMCID: PMC442531, DOI: 10.1172/jci113389.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityNude miceHumoral hypercalcemiaSyndrome of HHMMean tumor sizeReliable predictive markersHuman renal carcinoma cell linesCyclase-stimulating activityCell linesRenal carcinoma cell linesCultured tumor cellsHypercalcemic animalsTumor sizeCarcinoma cell linesPredictive markerBone resorptionTumor extractsTumor cellsMiceNormocalcemic animalsPotential mediatorsHypercalcemiaHHMCell assayMalignancyHumoral Hypercalcemia of Malignancy in Canine Lymphosarcoma*
Weir EC, Norrdin RW, Matus RE, Brooks MB, Broadus AE, Mitnick M, Johnston SD, Insogna KL. Humoral Hypercalcemia of Malignancy in Canine Lymphosarcoma*. Endocrinology 1988, 122: 602-608. PMID: 2828006, DOI: 10.1210/endo-122-2-602.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityHypercalcemic dogsCanine lymphosarcomaFractional phosphorus excretionEvidence of tumorQuantitative bone histomorphometryBone-resorbing factorsImmunoreactive PTH levelsPathogenesis of hypercalcemiaIliac crest biopsiesTumor tissue extractsCyclase-stimulating activityBone histomorphometric findingsCalcium excretionPTH levelsHumoral hypercalcemiaDihydroxyvitamin DBone resorptionHistomorphometric findingsBone histomorphometryPTH receptorBiopsy siteCrest biopsiesTumor extractsCompensatory increaseSynthetic human parathyroid hormone-like protein stimulates bone resorption and causes hypercalcemia in rats.
Stewart AF, Mangin M, Wu T, Goumas D, Insogna KL, Burtis WJ, Broadus AE. Synthetic human parathyroid hormone-like protein stimulates bone resorption and causes hypercalcemia in rats. Journal Of Clinical Investigation 1988, 81: 596-600. PMID: 3339131, PMCID: PMC329608, DOI: 10.1172/jci113358.Peer-Reviewed Original ResearchConceptsBone-resorbing agentsMicrograms/hParathyroid hormone-like proteinFetal rat long bonesAdenylate cyclasePotent bone-resorbing agentHormone-like proteinRat long bonesMembrane adenylate cyclaseSerum calciumHumoral hypercalcemiaOsmotic minipumpsBone resorptionPotent agonistLong bonesSuch synthetic peptidesHypercalcemiaRatsDlVivo bioactivityCyclaseSynthetic peptidesAgentsMinipumpsPatients
1987
Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*
WU T, INSOGNA K, HOUGH L, MILSTONE L, STEWART A. Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*. The Journal Of Clinical Endocrinology & Metabolism 1987, 65: 105-109. PMID: 3034948, DOI: 10.1210/jcem-65-1-105.Peer-Reviewed Original ResearchConceptsPTH-like factorHumoral hypercalcemiaPTH analogsPTH-responsive adenylate cyclaseDermal fibroblast linesAdenylate cyclaseTumor-derived factorsAdenylate cyclase responseDermal fibroblastsAdenylate cyclase stimulationHuman dermal fibroblastsM bovine PTHDermal physiologyFibroblast linesHuman dermal fibroblast linesBovine PTHHigh prevalenceTumor extractsCyclase responseSkin-derived fibroblastsCyclase stimulationPTHKeratinocyte-conditioned mediumHuman tumorsApparent potency
1983
Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy.
Stewart AF, Insogna KL, Goltzman D, Broadus AE. Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1454-1458. PMID: 6298791, PMCID: PMC393616, DOI: 10.1073/pnas.80.5.1454.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityCyclase-stimulating activityExtracts of tumorsHumoral hypercalcemiaPTH receptorTumor extractsPTH-sensitive adenylate cyclaseAdenylate cyclaseSensitive cytochemical bioassayRenal PTH receptorsParathyroid hormoneClinical studiesPTH resultsCompetitive inhibition studiesCytochemical bioassayTumorsPatientsHypercalcemiaGlucose-6-phosphate dehydrogenaseHHMFurther studiesMalignancyPTHFragment analogSpecific binding