2010
Circulating Levels of Soluble Klotho and FGF23 in X-Linked Hypophosphatemia: Circadian Variance, Effects of Treatment, and Relationship to Parathyroid Status
Carpenter TO, Insogna KL, Zhang JH, Ellis B, Nieman S, Simpson C, Olear E, Gundberg CM. Circulating Levels of Soluble Klotho and FGF23 in X-Linked Hypophosphatemia: Circadian Variance, Effects of Treatment, and Relationship to Parathyroid Status. The Journal Of Clinical Endocrinology & Metabolism 2010, 95: e352-e357. PMID: 20685863, PMCID: PMC2968736, DOI: 10.1210/jc.2010-0589.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAge FactorsAgedBone Density Conservation AgentsCalcitriolChildCircadian RhythmEnzyme-Linked Immunosorbent AssayFamilial Hypophosphatemic RicketsFemaleFibroblast Growth Factor-23Fibroblast Growth FactorsGenetic Diseases, X-LinkedGlucuronidaseHumansKlotho ProteinsMaleMiddle AgedParathyroid HormonePhosphatesVitamin DConceptsSerum KlothoSerum FGF23Higher klotho levelsHospital research unitRenal phosphate handlingAcademic medical centerEffect of treatmentFibroblast growth factorKlotho levelsPTH secretionMedical therapySoluble KlothoDihydroxyvitamin DFGF23 regulationPhosphate handlingMedical CenterFGF23KlothoXLHCircadian variationGrowth factorPTHAdultsHypophosphatemiaTherapy
2004
The Effect of Aging on the Skeletal Response to Intermittent Treatment with Parathyroid Hormone
Knopp E, Troiano N, Bouxsein M, Sun BH, Lostritto K, Gundberg C, Dziura J, Insogna K. The Effect of Aging on the Skeletal Response to Intermittent Treatment with Parathyroid Hormone. Endocrinology 2004, 146: 1983-1990. PMID: 15618351, DOI: 10.1210/en.2004-0770.Peer-Reviewed Original ResearchConceptsBody bone mineral densitySkeletal responseAged animalsIntermittent treatmentTotal body bone mineral densityYoung adult C57BL/6 miceDaily sc injectionsVehicle-treated animalsTrabecular bone volume fractionYoung adult miceBone mineral densityYoung adult animalsVertebral histomorphometryEffect of ageBone volume fractionParathyroid hormoneC57BL/6 miceSC injectionPTH treatmentMineral densityBody weightOsteoblast numberPTHCultured marrowYounger counterparts
2001
IL-6 negatively regulates IL-11 production in vitro and in vivo.
Nakchbandi I, Mitnick M, Masiukiewicz U, Sun B, Insogna K. IL-6 negatively regulates IL-11 production in vitro and in vivo. Endocrinology 2001, 142: 3850-6. PMID: 11517162, DOI: 10.1210/endo.142.9.8368.Peer-Reviewed Original ResearchConceptsIL-11 productionIL-6IL-11Bone resorptionOsteoclast formationIL-6 levelsIL-11 levelsFive-day infusionPTH excessPrimary hyperparathyroidismSerum levelsResorptive responseParathyroid adenomectomyPretreatment of cellsHuman PTHNonimmune serumPTHEffect of pretreatmentMRNA levelsSteady-state mRNA levelsPatientsCytokinesHuman osteoblast-like cellsResorptionSaos-2 cellsParathyroid hormone induces hepatic production of bioactive interleukin-6 and its soluble receptor
Mitnick M, Grey A, Masiukiewicz U, Bartkiewicz M, Rios-Velez L, Friedman S, Xu L, Horowitz M, Insogna K. Parathyroid hormone induces hepatic production of bioactive interleukin-6 and its soluble receptor. AJP Endocrinology And Metabolism 2001, 280: e405-e412. PMID: 11171594, DOI: 10.1152/ajpendo.2001.280.3.e405.Peer-Reviewed Original ResearchConceptsInterleukin-6IL-6sRParathyroid hormoneSoluble receptorBioactive interleukin-6Hepatic IL-6 productionBioactive IL-6IL-6 productionType 1 receptorHepatic stellate cellsDose-dependent productionIsolated rat liverHepatic endothelial cellsIL-6sR.Serum levelsBone resorptionHepatic productionEndocrine effectsKupffer cellsStellate cellsPTHImportant mediatorEndothelial cellsLiverHormone
1996
Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein
Weir E, Lowik C, Paliwal I, Insogna K. Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein. Journal Of Bone And Mineral Research 1996, 11: 1474-1481. PMID: 8889847, DOI: 10.1002/jbmr.5650111014.Peer-Reviewed Original ResearchConceptsBone resorptionMature osteoclastsParathyroid hormoneOsteoclast precursorsFetal rat long bonesParathyroid hormone-related proteinResistant acid phosphatase-positive osteoclastsMitogenic activityFactor 1Hormone-related proteinOrgan cultureCSF-1Rat long bonesTreatment of boneSignificant increaseOrgan culture assayHistological examinationBone assayOsteoclast recruitmentOsteoclast formationRat osteoclastsColony stimulating factor 1CSF-1 productionPTHResorption
1994
Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells.
Eielson C, Kaplan D, Mitnick MA, Paliwal I, Insogna K. Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells. Endocrinology 1994, 135: 1639-44. PMID: 7925127, DOI: 10.1210/endo.135.4.7925127.Peer-Reviewed Original ResearchEstrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells
Eielson C, Kaplan D, Mitnick M, Paliwal I, Insogna K. Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells. Endocrinology 1994, 135: 1639-1644. DOI: 10.1210/en.135.4.1639.Peer-Reviewed Original Research
1993
Macrophage colony‐stimulating factor release and receptor expression in bone cells
Weir E, Horowitz M, Baron R, Centrella M, Kacinski B, Insogna K. Macrophage colony‐stimulating factor release and receptor expression in bone cells. Journal Of Bone And Mineral Research 1993, 8: 1507-1518. PMID: 8304053, DOI: 10.1002/jbmr.5650081214.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowBone Marrow CellsCell DivisionCells, CulturedColony-Forming Units AssayCulture Media, ConditionedGranulocyte-Macrophage Colony-Stimulating FactorMacrophage Colony-Stimulating FactorMacrophagesMaleOsteoblastsOsteoclastsParathyroid HormoneParathyroid Hormone-Related ProteinProteinsRatsRats, Sprague-DawleyRats, WistarReceptor, Macrophage Colony-Stimulating FactorTumor Cells, CulturedConceptsColony-stimulating factorColony-stimulating activityM-CSFBone resorptionReceptor expressionMature osteoclastsPreincubation of CMColony-stimulating factor releaseHormone-related peptideM-CSF receptor c-FmsMacrophage colony-stimulating factorOsteoblast-osteoclast interactionsM-CSF mRNA levelsBone marrow cellsCSF-1 receptor expressionRat bone marrow cellsReceptor c-fmsROS 17/2.8 cellsParacrine roleImmunohistochemical techniquesRat osteoblastsPTHrP treatmentMarrow cellsFactor releasePTHHuman granulosa-luteal cells secrete parathyroid hormone-related protein in vivo and in vitro.
Gutmann JN, Burtis WJ, Dreyer BE, Andrade-Gordon P, Penzias AS, Polan ML, Insogna KL. Human granulosa-luteal cells secrete parathyroid hormone-related protein in vivo and in vitro. The Journal Of Clinical Endocrinology & Metabolism 1993, 76: 1314-1318. PMID: 8496323, DOI: 10.1210/jcem.76.5.8496323.Peer-Reviewed Original ResearchConceptsGranulosa-luteal cellsOvarian follicular fluidImmunoradiometric assayHuman granulosa-luteal cellsHuman ovarian follicular fluidCultured granulosa-luteal cellsParathyroid hormone-related proteinSubsequent prospective analysisAdenylate cyclase-stimulating activityHormone-related proteinCyclase-stimulating activityPresence of mRNAPossible paracrineShort-term culturePTHrP levelsNormal mammalian tissuesBasal secretionImmunohistochemical studyFollicular fluidAutocrine rolePTHrP immunoreactivityProspective analysisPTHrPPTHImmunoreactivity
1989
Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties.
Insogna KL, Stewart AF, Morris CA, Hough LM, Milstone LM, Centrella M. Native and a synthetic analogue of the malignancy-associated parathyroid hormone-like protein have in vitro transforming growth factor-like properties. Journal Of Clinical Investigation 1989, 83: 1057-1060. PMID: 2537846, PMCID: PMC303783, DOI: 10.1172/jci113947.Peer-Reviewed Original ResearchConceptsGrowth factor-like propertiesParathyroid hormone-like proteinHormone-like proteinNRK-49F cellsParaneoplastic syndromeHumoral hypercalcemiaLatter hormoneHuman tumorsPhysiological actionsPTHHuman dermal fibroblastsSignificant increaseSoft agarDisplay actionsDermal fibroblastsSynthetic peptidesSynthetic analoguesHypercalcemiaMalignancySyndromeTumorsUnique primary structureOsteoblast-Like Cells Secrete Granulocyte-Macrophage Colony-Stimulating Factor in Response to Parathyroid Hormone and Lipopolysaccharide*
WEIR EC, INSOGNA KL, HOROWITZ MC. Osteoblast-Like Cells Secrete Granulocyte-Macrophage Colony-Stimulating Factor in Response to Parathyroid Hormone and Lipopolysaccharide*. Endocrinology 1989, 124: 899-904. PMID: 2643512, DOI: 10.1210/endo-124-2-899.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DivisionCell LineColony-Stimulating FactorsDNA ReplicationGranulocyte-Macrophage Colony-Stimulating FactorGrowth SubstancesInterleukin-2Interleukin-3Interleukin-4InterleukinsKineticsLipopolysaccharidesOsteoblastsOsteosarcomaParathyroid HormoneRatsRecombinant ProteinsThymidineConceptsGranulocyte-macrophage colony-stimulating factorInterleukin-2GM-CSF antibodyIL-4Colony-stimulating factorMitogenic activityCSF antibodiesROS cellsGM-CSFT-cell mitogenic activityPresence of antibodiesDose-dependent increaseHT-2 cellsBone resorptionOsteoblast-like cellsOsteoclast precursorsPTHCell CMLipopolysaccharideAntibodiesCellular mechanismsEffect of incubationCytokinesBiochemical criteriaCSFSynthesis of a Gene Encoding Parathyroid Hormone-Like Protein-(1–141): Purification and Biological Characterization of the Expressed Protein*
THORIKAY M, KRAMER S, REYNOLDS F, SORVILLO J, DOESCHER L, WU T, MORRIS C, BURTIS W, INSOGNA K, VALENZUELA D, STEWART A. Synthesis of a Gene Encoding Parathyroid Hormone-Like Protein-(1–141): Purification and Biological Characterization of the Expressed Protein*. Endocrinology 1989, 124: 111-118. PMID: 2535801, DOI: 10.1210/endo-124-1-111.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBiological AssayBone and BonesCalciumCloning, MolecularCyclic AMPDNADogsEscherichia coliGenes, SyntheticKidneyMolecular Sequence DataMolecular WeightNeoplasm ProteinsOsteosarcomaParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsPlasmidsRatsRecombinant ProteinsTumor Cells, Cultured
1988
Identification of transcripts encoding a parathyroid hormone-like peptide in messenger RNAs from a variety of human and animal tumors associated with humoral hypercalcemia of malignancy.
Ikeda K, Mangin M, Dreyer BE, Webb AC, Posillico JT, Stewart AF, Bander NH, Weir EC, Insogna KL, Broadus AE. Identification of transcripts encoding a parathyroid hormone-like peptide in messenger RNAs from a variety of human and animal tumors associated with humoral hypercalcemia of malignancy. Journal Of Clinical Investigation 1988, 81: 2010-2014. PMID: 2454953, PMCID: PMC442656, DOI: 10.1172/jci113551.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularHumansHypercalcemiaKidney NeoplasmsMolecular Sequence DataNeoplasm ProteinsNucleic Acid HybridizationParaneoplastic SyndromesParathyroid HormoneParathyroid Hormone-Related ProteinPoly ARNARNA, MessengerRNA, NeoplasmSequence Homology, Nucleic AcidTranscription, GeneticConceptsPTH-like peptideParathyroid hormone-like peptideAnimal tumorsHumoral hypercalcemiaHormone-like peptideSyndrome of HHMVariety of humanHuman PTH-like peptideSimilar histological typeHistological typeHuman PTHTumor typesTumorsHHMHypercalcemiaMalignancySyndromeMessenger RNAMRNAWide spectrumHumansPeptidesMammalian speciesRNAPTH
1987
Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*
WU T, INSOGNA K, HOUGH L, MILSTONE L, STEWART A. Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*. The Journal Of Clinical Endocrinology & Metabolism 1987, 65: 105-109. PMID: 3034948, DOI: 10.1210/jcem-65-1-105.Peer-Reviewed Original ResearchConceptsPTH-like factorHumoral hypercalcemiaPTH analogsPTH-responsive adenylate cyclaseDermal fibroblast linesAdenylate cyclaseTumor-derived factorsAdenylate cyclase responseDermal fibroblastsAdenylate cyclase stimulationHuman dermal fibroblastsM bovine PTHDermal physiologyFibroblast linesHuman dermal fibroblast linesBovine PTHHigh prevalenceTumor extractsCyclase responseSkin-derived fibroblastsCyclase stimulationPTHKeratinocyte-conditioned mediumHuman tumorsApparent potencyCO-PURIFICATION OF TRANSFORMING GROWTH FACTOR LIKE ACTIVITY WITH PTH-LIKE AND BONE-RESORBING ACTIVITIES FROM A TUMOR ASSOCIATED WITH HUMORAL HYPERCALCEMIA OF MALIGNANCY
INSOGNA K, WEIR E, WU T, STEWART A, BROADUS A, BURTIS W, CENTRELLA M. CO-PURIFICATION OF TRANSFORMING GROWTH FACTOR LIKE ACTIVITY WITH PTH-LIKE AND BONE-RESORBING ACTIVITIES FROM A TUMOR ASSOCIATED WITH HUMORAL HYPERCALCEMIA OF MALIGNANCY. Endocrinology 1987, 120: 2183-2185. PMID: 3494596, DOI: 10.1210/endo-120-5-2183.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityBone-resorbing factorsBone-resorbing activityHumoral hypercalcemiaGrowth factor beta-like activityPTH-like proteinLeydig cell tumorReverse-phase HPLC stepsBeta-like activityCyclase-stimulating activityGrowth factor betaCell tumorsResponsible mediatorsGrowth factor activityTumor extractsFisher ratsAnimal modelsFactor betaTumor tissueSingle fractionGrowth factorHypercalcemiaMalignancyPTHSequential treatment
1986
Two Species of Adenylate Cyclase-Stimulating Activity in a Murine Squamous Carcinoma Model of Humoral Hypercalcemia of Malignancy*
BURTIS W, BROADUS A, INSOGNA K, WEIR E, STEWART A. Two Species of Adenylate Cyclase-Stimulating Activity in a Murine Squamous Carcinoma Model of Humoral Hypercalcemia of Malignancy*. Endocrinology 1986, 118: 1982-1988. PMID: 3009144, DOI: 10.1210/endo-118-5-1982.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsBiological AssayCarcinoma, Squamous CellChromatography, High Pressure LiquidDisease Models, AnimalDogsHypercalcemiaKidney CortexMiceNeoplasm ProteinsOsteosarcomaParathyroid HormonePeptide FragmentsRatsReceptors, Cell SurfaceReceptors, Parathyroid HormoneSkin NeoplasmsConceptsAdenylate cyclase-stimulating activityCyclase-stimulating activityTumor extractsPTH antagonistHumoral hypercalcemiaRat osteosarcomaCarcinoma modelDose-response curve parallelRat Leydig cellsAdenylate cyclase activityAdenylate cyclase systemDose-response curvePostreceptor stepPTH (3-34) amideLeydig cellsReversed-phase HPLCCyclase systemCyclase activityPeak IICommon mediatorPTHCyclase assaysHypercalcemiaMalignancyPhase HPLC
1983
Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy.
Stewart AF, Insogna KL, Goltzman D, Broadus AE. Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1454-1458. PMID: 6298791, PMCID: PMC393616, DOI: 10.1073/pnas.80.5.1454.Peer-Reviewed Original ResearchConceptsAdenylate cyclase-stimulating activityCyclase-stimulating activityExtracts of tumorsHumoral hypercalcemiaPTH receptorTumor extractsPTH-sensitive adenylate cyclaseAdenylate cyclaseSensitive cytochemical bioassayRenal PTH receptorsParathyroid hormoneClinical studiesPTH resultsCompetitive inhibition studiesCytochemical bioassayTumorsPatientsHypercalcemiaGlucose-6-phosphate dehydrogenaseHHMFurther studiesMalignancyPTHFragment analogSpecific binding