2022
Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1
Kaur H, Garber L, Murphy JW, Vinetz JM. Structure–function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1. Protein Science 2022, 31: e4289. PMID: 35481637, PMCID: PMC8994504, DOI: 10.1002/pro.4289.Peer-Reviewed Original ResearchConceptsPutative chitin-binding domainChitin-binding domainPlasmodium ookinetesProtein-protein interactionsRecombinant protein productionIntermolecular protein-protein interactionsMosquito midgut invasionStructure-function analysisEnzymatic activityIntramolecular disulfide linkagesIntermolecular disulfide bondingChitin-containing peritrophic matrixParasite cladesMidgut invasionPeritrophic matrixCysteine residuesProtein productionChitinase activityBiochemical studiesDisulfide bondingUnpaired cysteineChitinaseMalaria parasitesE. coliMutants
2021
A Hetero-Multimeric Chitinase-Containing Plasmodium falciparum and Plasmodium gallinaceum Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion
Patra KP, Kaur H, Kolli SK, Wozniak JM, Prieto JH, Yates JR, Gonzalez DJ, Janse CJ, Vinetz JM. A Hetero-Multimeric Chitinase-Containing Plasmodium falciparum and Plasmodium gallinaceum Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion. Frontiers In Cellular And Infection Microbiology 2021, 10: 615343. PMID: 33489941, PMCID: PMC7821095, DOI: 10.3389/fcimb.2020.615343.Peer-Reviewed Original ResearchConceptsChitin-containing peritrophic matrixHMW complexesMosquito midgut invasionDomain-related proteinNext-generation vaccinesMicronemal proteinsVon Willebrand factorProtein complexesMidgut invasionPeritrophic matrixProteolytic milieuMidgut epitheliumPfCHT1Malaria parasitesSurface enolaseOokinetesPlasmodium falciparumWillebrand factorMidgut wallAdhesive proteinsProteinMass spectrometryShort formSize exclusion chromatography dataMosquito vectors
2010
Apical Surface Expression of Aspartic Protease Plasmepsin 4, a Potential Transmission-blocking Target of the Plasmodium Ookinete*
Li F, Patra KP, Yowell CA, Dame JB, Chin K, Vinetz JM. Apical Surface Expression of Aspartic Protease Plasmepsin 4, a Potential Transmission-blocking Target of the Plasmodium Ookinete*. Journal Of Biological Chemistry 2010, 285: 8076-8083. PMID: 20056606, PMCID: PMC2832958, DOI: 10.1074/jbc.m109.063388.Peer-Reviewed Original ResearchConceptsPlasmepsin 4Midgut invasionAspartic proteasesDigestive vacuoleMidgut peritrophic matrixApical surface expressionChitin-binding proteinsMass spectrometry sequencingMalaria parasitesPeritrophic matrixPlasmodium invasionAspartic protease inhibitorsPlasmodium ookinetesParasite infectivityApical surfaceDefinitive hostsMechanistic roleAffinity columnBlood-stage PlasmodiumSurface expressionCalpain inhibitorsMidgut basal laminaProteinVaccine targetsVacuoles
2001
Knockout of the Rodent Malaria Parasite Chitinase PbCHT1 Reduces Infectivity to Mosquitoes
Dessens J, Mendoza J, Claudianos C, Vinetz J, Khater E, Hassard S, Ranawaka G, Sinden R. Knockout of the Rodent Malaria Parasite Chitinase PbCHT1 Reduces Infectivity to Mosquitoes. Infection And Immunity 2001, 69: 4041-4047. PMID: 11349074, PMCID: PMC98467, DOI: 10.1128/iai.69.6.4041-4047.2001.Peer-Reviewed Original ResearchConceptsPeritrophic matrixChitinase geneChitinase activityHuman malaria parasite Plasmodium falciparumMalaria parasite Plasmodium falciparumPlasmodium-mosquito interactionsAvian malaria parasite Plasmodium gallinaceumAvian malaria transmissionParasite Plasmodium falciparumMalaria parasite Plasmodium gallinaceumChitin-containing structuresNull mutantsRodent malaria parasiteInfected blood mealMalaria ookinetesTransmission-blocking drugsAnopheles stephensi mosquitoesMidgut invasionStructural orthologsMosquito midgutOokinete invasionPM disruptionMidgut epitheliumTargeted disruptionMalaria transmission
2000
Chitinases of the Avian Malaria Parasite Plasmodium gallinaceum, a Class of Enzymes Necessary for Parasite Invasion of the Mosquito Midgut*
Vinetz J, Valenzuela J, Specht C, Aravind L, Langer R, Ribeiro J, Kaslow D. Chitinases of the Avian Malaria Parasite Plasmodium gallinaceum, a Class of Enzymes Necessary for Parasite Invasion of the Mosquito Midgut*. Journal Of Biological Chemistry 2000, 275: 10331-10341. PMID: 10744721, DOI: 10.1074/jbc.275.14.10331.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsChickensChitinasesConsensus SequenceCulicidaeDigestive SystemEpithelial CellsGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansKineticsMalaria, AvianMolecular Sequence DataPlasmodium gallinaceumRecombinant ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsAvian malaria parasite Plasmodium gallinaceumMalaria parasite Plasmodium gallinaceumChitin-containing peritrophic matrixMosquito midgutPlasmodium gallinaceumTransmission-blocking interventionsBlood mealPgCHT1Mosquito midgut invasionPlasmodium ookinetesMidgut invasionParasite invasionPotential targetPeritrophic matrixMidgut epitheliumInvasionParasitesGallinaceumOokinetesMealMicroMActivity profiles