Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
Chopyk J, Chattopadhyay S, Kulkarni P, Claye E, Babik K, Reid M, Smyth E, Hittle L, Paulson J, Cruz-Cano R, Pop M, Buehler S, Clark P, Sapkota A, Mongodin E. Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens. Microbiome 2017, 5: 22. PMID: 28202080, PMCID: PMC5312438, DOI: 10.1186/s40168-017-0235-0.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaBlack or African AmericanDNA, BacterialHumansMentholMicrobiotaPolymerase Chain ReactionPseudomonasRNA, Ribosomal, 16SSmokingTobacco ProductsConceptsQuantitative Insights Into Microbial EcologyResistant to harsh environmental conditionsBacterial pathogensHarsh environmental conditionsV3-V4 hypervariable regionIllumina MiSeq platformHuman bacterial pathogensBacterial community profilesBacterial community richnessPotential bacterial pathogensEnvironmental conditionsRRNA geneMiSeq platformAbundant generaBacterial microbiotaBacterial communitiesMicrobial ecologyGenomic DNACommunity richnessBacterial compositionHypervariable regionPCR amplificationCommunity profilesPseudomonas putidaLysis protocol