Featured Publications
Differential abundance analysis for microbial marker-gene surveys
Paulson J, Stine O, Bravo H, Pop M. Differential abundance analysis for microbial marker-gene surveys. Nature Methods 2013, 10: 1200-1202. PMID: 24076764, PMCID: PMC4010126, DOI: 10.1038/nmeth.2658.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsArea Under CurveCluster AnalysisComputer SimulationDatabases, GeneticGene Expression ProfilingGenetic MarkersGenetic VariationHumansIntestinesMetagenomicsMiceMicrobiotaModels, GeneticModels, StatisticalNormal DistributionPhenotypeRNA, Ribosomal, 16SSequence Analysis, DNASoftware
2018
An Introduced Crop Plant Is Driving Diversification of the Virulent Bacterial Pathogen Erwinia tracheiphila
Shapiro L, Paulson J, Arnold B, Scully E, Zhaxybayeva O, Pierce N, Rocha J, Klepac-Ceraj V, Holton K, Kolter R. An Introduced Crop Plant Is Driving Diversification of the Virulent Bacterial Pathogen Erwinia tracheiphila. MBio 2018, 9: 10.1128/mbio.01307-18. PMID: 30279283, PMCID: PMC6168856, DOI: 10.1128/mbio.01307-18.Peer-Reviewed Original ResearchConceptsTemperate eastern North AmericaEastern North AmericaErwinia tracheiphila</i>,Host plant speciesPlant speciesCrop plantsCausal agent of bacterial wiltAgent of bacterial wiltVirulence gene repertoireLow genetic heterogeneityLarge-scale sequencingSusceptible to isolatesNorth AmericaDriving diversificationPhylogenomic analysisPopulation bottlenecksGenetic clustersEcological traitsIsolate collectionGene repertoirePopulation expansionGeographic rangeEvolutionary trajectoriesGenetic heterogeneityCausal agent
2017
Exploring regulation in tissues with eQTL networks
Fagny M, Paulson J, Kuijjer M, Sonawane A, Chen C, Lopes-Ramos C, Glass K, Quackenbush J, Platig J. Exploring regulation in tissues with eQTL networks. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e7841-e7850. PMID: 28851834, PMCID: PMC5604022, DOI: 10.1073/pnas.1707375114.Peer-Reviewed Original ResearchConceptsExpression quantitative trait lociEQTL networksGenetic variantsCoherent biological processesImpact of genetic variantsActive chromatin regionsQuantitative trait lociTissue-specific processesGene expression levelsChromatin regionsGroup genesTrait lociComplex phenotypesRegulatory potentialGenotype to phenotype mappingPhenotype mappingBiological processesRegulatory functionsExpression levelsGenesRegulatory impactVariantsLociBipartite networksPhenotypeMinimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing
Garrine M, Mandomando I, Vubil D, Nhampossa T, Acacio S, Li S, Paulson J, Almeida M, Domman D, Thomson N, Alonso P, Stine O. Minimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing. PLOS Neglected Tropical Diseases 2017, 11: e0005671. PMID: 28622368, PMCID: PMC5489214, DOI: 10.1371/journal.pntd.0005671.Peer-Reviewed Original ResearchConceptsMultilocus variable-number tandem-repeat analysisWhole-genome sequencingTandem repeat analysisGenome sequenceMultilocus variable number tandem repeat analysisVariable number tandem repeat analysisVariable-number tandem repeat analysisCholera toxin geneVibrio cholerae isolatesPresence of recombinationMinimal genetic changeMLVA lociCholerae isolatesSeventh pandemicGenetic relatednessClonal complexesToxin genesMolecular clockVibrio choleraeClinical isolatesIdentical isolatesGenetic changesRepeat analysisEnvironmental reservoirsIsolates