2022
Ubiquitin-like processing of TUG proteins as a mechanism to regulate glucose uptake and energy metabolism in fat and muscle
Bogan JS. Ubiquitin-like processing of TUG proteins as a mechanism to regulate glucose uptake and energy metabolism in fat and muscle. Frontiers In Endocrinology 2022, 13: 1019405. PMID: 36246906, PMCID: PMC9556833, DOI: 10.3389/fendo.2022.1019405.Peer-Reviewed Original ResearchConceptsGolgi matrixTUG ProteinVesicle cargoC-terminal productInsulin stimulationN-degron pathwayGLUT4 storage vesiclesCell surfaceUbiquitin-like proteinGLUT4 glucose transportersGlucose uptakeAspects of physiologyN-terminal cleavage productMuscle cellsP97 ATPaseCleavage productsC-terminusFatty acid oxidationGene expressionSingle proteinN-terminusMatrix proteinsEndoproteolytic cleavageCell typesGlucose transporterInhibitors of RNA and protein synthesis cause Glut4 translocation and increase glucose uptake in adipocytes
Meriin AB, Zaarur N, Bogan JS, Kandror KV. Inhibitors of RNA and protein synthesis cause Glut4 translocation and increase glucose uptake in adipocytes. Scientific Reports 2022, 12: 15640. PMID: 36123369, PMCID: PMC9485115, DOI: 10.1038/s41598-022-19534-5.Peer-Reviewed Original ResearchConceptsInhibitors of RNAGLUT4 translocationProtein synthesisEndocytosis of GLUT4Glucose uptakeRecycling of transferrinGlucose transporter 4Actinomycin DBiosynthesis de novoSignaling proteinsPlasma membraneTransporter 4Intracellular retentionContinuous RNATranslocationRNADe novoAdipocytesVesiclesInhibitorsTBC1D4UptakeEndocytosisGLUT4Exocytosis
2021
Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake
Habtemichael EN, Li DT, Camporez JP, Westergaard XO, Sales CI, Liu X, López-Giráldez F, DeVries SG, Li H, Ruiz DM, Wang KY, Sayal BS, González Zapata S, Dann P, Brown SN, Hirabara S, Vatner DF, Goedeke L, Philbrick W, Shulman GI, Bogan JS. Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake. Nature Metabolism 2021, 3: 378-393. PMID: 33686286, PMCID: PMC7990718, DOI: 10.1038/s42255-021-00359-x.Peer-Reviewed Original ResearchConceptsTUG cleavageGlucose uptakeProtein degradation pathwaysGLUT4 glucose transportersCoactivator PGC-1αC-terminal cleavage productInsulin-stimulated glucose uptakeAte1 arginyltransferaseGene expressionPhysiological relevanceWhole-body energy expenditureGlucose transporterPeroxisome proliferator-activated receptorCell surfacePGC-1αProtein 1Proliferator-activated receptorDegradation pathwayEffect of insulinCleavage pathwayAdipose cellsCleavage productsPathwayCleavageEnergy expenditure
2018
Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes
Habtemichael EN, Li DT, Alcázar-Román A, Westergaard XO, Li M, Petersen MC, Li H, DeVries SG, Li E, Julca-Zevallos O, Wolenski JS, Bogan JS. Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes. Journal Of Biological Chemistry 2018, 293: 10466-10486. PMID: 29773651, PMCID: PMC6036200, DOI: 10.1074/jbc.ra118.003021.Peer-Reviewed Original ResearchMeSH KeywordsAdipocytesAnimalsCarrier ProteinsCell MembraneCells, CulturedGlucoseGlucose Transporter Type 4Hypoglycemic AgentsInsulinIntracellular Signaling Peptides and ProteinsKinesinsMaleMiceMice, Inbred C57BLMotor ActivityProtein TransportProteolysisRatsRats, Sprague-DawleySignal TransductionUbiquitinUbiquitin ThiolesteraseConceptsGLUT4 storage vesiclesTUG cleavageGolgi matrixPlasma membraneGLUT4 glucose transporter translocationMicrotubule-based movementUbiquitin-like proteinGLUT4 glucose transportersStorage vesiclesGlucose transporter translocationAttenuation of insulinKinesin motor proteinsGLUT4 vesiclesSpecialized vesiclesGLUT4 translocationTransporter translocationSplice formsMotor proteinsProtein trapProtein abundanceProteolytic pathwayDiet-induced insulin resistanceEndoproteolytic cleavageGlucose transporterProteolytic processing
2015
Coordinated Regulation of Vasopressin Inactivation and Glucose Uptake by Action of TUG Protein in Muscle*
Habtemichael EN, Alcázar-Román A, Rubin BR, Grossi LR, Belman JP, Julca O, Löffler MG, Li H, Chi NW, Samuel VT, Bogan JS. Coordinated Regulation of Vasopressin Inactivation and Glucose Uptake by Action of TUG Protein in Muscle*. Journal Of Biological Chemistry 2015, 290: 14454-14461. PMID: 25944897, PMCID: PMC4505512, DOI: 10.1074/jbc.c115.639203.Peer-Reviewed Original ResearchConceptsInsulin-regulated aminopeptidaseVesicle translocationGLUT4 intracellular retentionGlucose uptakeT-tubule fractionTransgenic miceTransmembrane aminopeptidaseAQP2 water channelsTUG ProteinCoordinated regulationGLUT4 translocationInsulin stimulationPhysiological importanceGlucose transporterProteolytic processingIntracellular retentionTranslocationWater homeostasisMuscle cellsGLUT4Skeletal muscleShort peptidesProteolysisProteinAbundanceAcetylation of TUG Protein Promotes the Accumulation of GLUT4 Glucose Transporters in an Insulin-responsive Intracellular Compartment*
Belman JP, Bian RR, Habtemichael EN, Li DT, Jurczak MJ, Alcázar-Román A, McNally LJ, Shulman GI, Bogan JS. Acetylation of TUG Protein Promotes the Accumulation of GLUT4 Glucose Transporters in an Insulin-responsive Intracellular Compartment*. Journal Of Biological Chemistry 2015, 290: 4447-4463. PMID: 25561724, PMCID: PMC4326849, DOI: 10.1074/jbc.m114.603977.Peer-Reviewed Original ResearchMeSH Keywords3T3-L1 CellsAcetylationAdipocytesAnimalsBlotting, WesternCarrier ProteinsCell MembraneCells, CulturedCystinyl AminopeptidaseCytoplasmFlow CytometryGlucoseGlucose Transporter Type 4HumansHypoglycemic AgentsImmunoprecipitationInsulinIntracellular Signaling Peptides and ProteinsMaleMiceMice, Inbred C57BLMice, KnockoutProtein TransportReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSirtuin 2ConceptsGLUT4 storage vesiclesGLUT4 glucose transportersInsulin-regulated aminopeptidaseGolgin-160Acetylated residuesC-terminusGolgi matrix proteinsSirtuin 2Insulin-responsive vesiclesGlucose transporterUnstimulated cellsGLUT4 traffickingInsulin-stimulated glucose uptakeGlucose uptakeC-terminal peptidePlasma membraneIntracellular compartmentsMatrix proteinsACBD3Protein promotesWild-type controlsDependent deacetylaseGLUT4Proteolytic processingIntracellular retention
2012
Endoproteolytic Cleavage of TUG Protein Regulates GLUT4 Glucose Transporter Translocation*
Bogan JS, Rubin BR, Yu C, Löffler MG, Orme CM, Belman JP, McNally LJ, Hao M, Cresswell JA. Endoproteolytic Cleavage of TUG Protein Regulates GLUT4 Glucose Transporter Translocation*. Journal Of Biological Chemistry 2012, 287: 23932-23947. PMID: 22610098, PMCID: PMC3390669, DOI: 10.1074/jbc.m112.339457.Peer-Reviewed Original Research3T3-L1 CellsAdipocytesAmino Acid SequenceAnimalsCarrier ProteinsGlucoseGlucose Transporter Type 4Golgi ApparatusGreen Fluorescent ProteinsHEK293 CellsHumansImmunoblottingInsulinIntracellular Signaling Peptides and ProteinsMiceMicroscopy, FluorescenceMolecular Sequence DataMutationProtein TransportProteolysisRNA InterferenceSequence Homology, Amino AcidRegulation of Glucose Transporter Translocation in Health and Diabetes
Bogan JS. Regulation of Glucose Transporter Translocation in Health and Diabetes. Annual Review Of Biochemistry 2012, 81: 507-532. PMID: 22482906, DOI: 10.1146/annurev-biochem-060109-094246.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsTrafficking pathwaysVesicle trafficking pathwaysAS160/TBC1D4GLUT4 glucose transportersGlucose transporter translocationRab GTPasesTrafficking itineraryTransporter translocationIntracellular membranesPlasma membraneInsulin signalingGlucose transporterCell surfaceStorage vesiclesUnstimulated cellsGlucose uptakeTranslocationVesiclesInsulin exposurePathwayFat cellsPathogenesis of diabetesMembraneGTPasesVAMP2