1999
Redesign and Genetic Dissection of The Rhabdoviruses
Roberts A, Rose J. Redesign and Genetic Dissection of The Rhabdoviruses. Advances In Virus Research 1999, 53: 301-319. PMID: 10582104, DOI: 10.1016/s0065-3527(08)60353-x.Peer-Reviewed Original ResearchConceptsNegative-strand RNA virusesGenetic dissectionNegative-strand genome RNAWild-type virusRNA virusesNonsegmented negative-strand RNA virusesBacteriophage T7 RNA polymeraseNegative-strand RNA genomeVaccine vector developmentT7 RNA polymeraseRNA polymeraseInfection of cellsT7 promoterGenome RNARNA genomeGenetic analysisPolymerase proteinNNS virusesViral transcriptionNotorious pathogenViral assemblyRhabdovirusDNA copiesRecombinant virusesPlasmid DNA
1996
The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus
Schnell M, Buonocore L, Whitt M, Rose J. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. Journal Of Virology 1996, 70: 2318-2323. PMID: 8642658, PMCID: PMC190073, DOI: 10.1128/jvi.70.4.2318-2323.1996.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceChloramphenicol O-AcetyltransferaseCloning, MolecularConserved SequenceGene Expression Regulation, ViralMembrane GlycoproteinsMolecular Sequence DataRegulatory Sequences, Nucleic AcidRNA, ViralSerial PassageTranscription, GeneticVesicular stomatitis Indiana virusViral Envelope ProteinsViral Plaque AssayConceptsForeign genesBacterial enzyme chloramphenicolNew transcription unitTranscription start sequenceWild-type titersTranscription unitFunctional chloramphenicolPolyadenylation signalEnzyme chloramphenicolCell biologyVesicular stomatitis virusVSV genomeIntergenic dinucleotideGene junctionStable expressionGlycoprotein geneGenesDNA copiesGlycoprotein mRNAInfectious VSVRecombinant virusesStomatitis virusStart sequenceRecombinant VSVSequenceNormal Replication of Vesicular Stomatitis Virus without C Proteins
KRETZSCHMAR E, PELUSO R, SCHNELL M, WHITT M, ROSE J. Normal Replication of Vesicular Stomatitis Virus without C Proteins. Virology 1996, 216: 309-316. PMID: 8607260, DOI: 10.1006/viro.1996.0066.Peer-Reviewed Original ResearchConceptsP geneWild-type virusNormal replicationSmall basic proteinP protein sequenceSingle base changeNew Jersey serotypeWild-type virus particlesInsect vectorsVesicular stomatitis virusC proteinStop codonViral mRNAsVSV growthInfectious cloneBase changesMutant virusProteinGenesStomatitis virusViral pathogenesisBasic proteinM proteinVirus particlesTissue culture
1981
Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli.
Rose J, Shafferman A. Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 6670-6674. PMID: 6273881, PMCID: PMC349111, DOI: 10.1073/pnas.78.11.6670.Peer-Reviewed Original ResearchConceptsGlycoprotein geneEscherichia coliNH2-terminal domainTryptic peptide mappingRegulatory regionsProtein sequencesBacterial plasmidsCOOH terminusGene regionG proteinsConditional expressionAmino acidsProteinHydrophobic segmentsPeptide mappingPlasmidStomatitis virusGenesDeletionColiLethalitySequenceExpressionAntigenic determinantsTerminusLocalized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription
Iverson L, Rose J. Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 1981, 23: 477-484. PMID: 6258804, DOI: 10.1016/0092-8674(81)90143-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCricetinaeGenes, ViralRNA, MessengerRNA, ViralTranscription, GeneticVesicular stomatitis Indiana virusConceptsIntergenic regionVesicular stomatitis virus transcriptionKinetics of transcriptionVesicular stomatitis virus mRNALevel of transcriptionDNA-RNA hybridizationTranscription terminationSequential transcriptionEucaryotic systemsVSV genomeGene expressionGene junctionTranscriptionDiscontinuous synthesisVirus mRNAVirus transcriptionG geneGenomeSuch pausesPolyadenylationGenesN-NSAttenuation mechanismMRNAHybridization