1989
Carboxy-terminal SEKDEL sequences retard but do not retain two secretory proteins in the endoplasmic reticulum.
Zagouras P, Rose J. Carboxy-terminal SEKDEL sequences retard but do not retain two secretory proteins in the endoplasmic reticulum. Journal Of Cell Biology 1989, 109: 2633-2640. PMID: 2592401, PMCID: PMC2115906, DOI: 10.1083/jcb.109.6.2633.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSEKDEL sequenceSecretory proteinsSequence Ser-GluAmino acidsMonkey COS cellsOligonucleotide-directed mutagenesisLast amino acidFirst amino acidProtein exitIndirect immunofluorescence microscopyAnimal cellsCOS cellsCOOH terminusAlpha subunitProtein structureGolgi apparatusLys-AspImmunofluorescence microscopyOligosaccharide processingProteinReticulumSEKDELSer-GluSpecific interactions
1985
Glycosylation allows cell-surface transport of an anchored secretory protein
Guan J, Machamer C, Rose J. Glycosylation allows cell-surface transport of an anchored secretory protein. Cell 1985, 42: 489-496. PMID: 3928168, DOI: 10.1016/0092-8674(85)90106-0.Peer-Reviewed Original ResearchConceptsCell surfaceProtein transportMutant proteinsCarboxy-terminal extensionCell surface transportVesicular stomatitis virus glycoproteinMembrane-anchored formSingle amino acidCytoplasmic domainHybrid geneGlycosylation sitesConsensus sequenceSecretory proteinsGolgi apparatusCellular membranesAmino acidsProteinRandom sitesGlycosylationVirus glycoproteinRat growth hormoneGrowth hormoneTransmembraneGenesSites
1984
Conversion of a secretory protein into a transmembrane protein results in its transport to the golgi complex but not to the cell surface
Guan J, Rose J. Conversion of a secretory protein into a transmembrane protein results in its transport to the golgi complex but not to the cell surface. Cell 1984, 37: 779-787. PMID: 6589049, DOI: 10.1016/0092-8674(84)90413-6.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsSecretory proteinsFusion proteinCell surfaceVesicular stomatitis virus glycoproteinRat growth hormoneMembrane spanningCytoplasmic domainCDNA clonesCarboxy terminusHybrid geneEucaryotic cellsTransmembrane configurationGolgi apparatusProtein resultsProteinMicrosomal membranesVirus glycoproteinRapid secretionMembranePalmitic acidGolgiGenesTerminus