1983
Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein.
Florkiewicz R, Smith A, Bergmann J, Rose J. Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein. Journal Of Cell Biology 1983, 97: 1381-1388. PMID: 6415065, PMCID: PMC2112694, DOI: 10.1083/jcb.97.5.1381.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virus glycoproteinMouse cell linesG proteinsNormal G proteinsStable mouse cell linesEndoplasmic reticulumCell linesTg proteinRough endoplasmic reticulumVesicular stomatitis virus G proteinCell surfaceVirus G proteinBovine papilloma virusVirus glycoproteinComplex oligosaccharidesAnchor sequenceLevel of expressionPSV2 vectorCDNA encodingAnchorless proteinAberrant splicingDNA fragmentsGolgi apparatusMRNA sequencesRate-limiting step
1982
Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells
Rose J, Bergmann J. Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells. Cell 1982, 30: 753-762. PMID: 6291783, DOI: 10.1016/0092-8674(82)90280-x.Peer-Reviewed Original ResearchConceptsG proteinsVesicular stomatitis virusCOS1 cellsCOOH terminusStomatitis virusMouse L cellsSV40 early promoterSV40 late promoterNormal G proteinsTransmembrane domainCDNA clonesEucaryotic cellsLate promoterEarly promoterPlasmid vectorCell typesAmino acidsProteinLipid bilayersL cellsPromoterTerminusG-DNADNACells