1996
Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.
Rodgers W, Rose J. Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains. Journal Of Cell Biology 1996, 135: 1515-1523. PMID: 8978819, PMCID: PMC2133949, DOI: 10.1083/jcb.135.6.1515.Peer-Reviewed Original ResearchConceptsExclusion of CD45Low kinase activityGEM fractionMembrane domainsKinase activitySrc family tyrosine kinasesReservoir of enzymesSpecific membrane domainsActivity of p56lckTyrosine phosphatase CD45Family tyrosine kinasesT cell developmentTX-100-soluble fractionGEM domainsPhosphatase CD45Membrane proteinsKinase specific activityLckTyrosine kinaseMembrane fractionJurkat cellsPeptide mappingP56lckPhosphorylationHyperphosphorylation
1994
Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction
Rodgers W, Crise B, Rose J. Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. DOI: 10.1128/mcb.14.8.5384-5391.1994.Peer-Reviewed Original ResearchGPI-anchored proteinsProtein tyrosine kinasesGlycolipid-enriched membraneP56 LckTyrosine kinaseMembrane fractionCys-3Glycosyl-phosphatidylinositol (GPI)-anchored membrane proteinsGlycolipid-enriched membrane fractionCys-5Src family protein tyrosine kinasesTriton X-100-insolubleAnalysis of mutantsGlycosyl-phosphatidylinositol-anchored proteinsN-terminal myristateAssociation of p56lckGPI anchorTransmembrane signalingMembrane proteinsMembrane domainsHeLa cellsKinaseLckProteinTarget signalSignals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.
Rodgers W, Crise B, Rose J. Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. PMID: 8035816, PMCID: PMC359057, DOI: 10.1128/mcb.14.8.5384.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCell CompartmentationDNA PrimersGlycolipidsGlycosylphosphatidylinositolsHeLa CellsHumansLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedMyristatesPalmitatesProteinsProtein-Tyrosine KinasesStructure-Activity RelationshipConceptsProtein tyrosine kinasesCertain protein tyrosine kinasesTyrosine kinaseMembrane fractionSrc family protein tyrosine kinasesFamily protein tyrosine kinasesAnalysis of mutantsN-terminal myristateCy-3Glycolipid-enriched membranesAssociation of p56lckCys-5Membrane domainsMembrane proteinsAnchored proteinsGPI anchorGlycosyl phosphatidylinositolKinaseP56lckCell typesHeLa cellsMDCK cellsGPIProteinGentle disruptionSignals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction
Rodgers W, Crise B, Rose J. Signals Determining Protein Tyrosine Kinase and Glycosyl-Phosphatidylinositol-Anchored Protein Targeting to a Glycolipid-Enriched Membrane Fraction. Molecular And Cellular Biology 1994, 14: 5384-5391. DOI: 10.1128/mcb.14.8.5384-5391.1994.Peer-Reviewed Original ResearchProtein tyrosine kinasesCertain protein tyrosine kinasesP56 lckTyrosine kinaseMembrane fractionGlycosyl phosphatidylinositolSrc family protein tyrosine kinasesFamily protein tyrosine kinasesAnalysis of mutantsN-terminal myristateCy-3Glycolipid-enriched membranesAssociation of p56lckCys-5Membrane domainsMembrane proteinsAnchored proteinsGPI anchorKinaseCell typesHeLa cellsLckMDCK cellsGPIProteinInteractions of normal and mutant vesicular stomatitis virus matrix proteins with the plasma membrane and nucleocapsids
Chong L, Rose J. Interactions of normal and mutant vesicular stomatitis virus matrix proteins with the plasma membrane and nucleocapsids. Journal Of Virology 1994, 68: 441-447. PMID: 8254754, PMCID: PMC236304, DOI: 10.1128/jvi.68.1.441-447.1994.Peer-Reviewed Original ResearchConceptsMembrane associationPlasma membraneAmino-terminal basic domainVesicular stomatitis virusWild-type M proteinCellular membranesMatrix proteinsVesicular stomatitis virus matrix proteinM proteinStable membrane associationTruncated M proteinsVirus matrix proteinVSV nucleocapsidsBasic domainAmino terminusNucleocapsid bindingNucleocapsid interactionMembrane fractionVSV proteinsAmino acidsMembrane specificityProteinStomatitis virusNucleocapsidsMembrane