Cell-surface expression of a membrane-anchored form of the human chorionic gonadotropin alpha subunit.
Guan J, Cao H, Rose J. Cell-surface expression of a membrane-anchored form of the human chorionic gonadotropin alpha subunit. Journal Of Biological Chemistry 1988, 263: 5306-5313. PMID: 2451667, DOI: 10.1016/s0021-9258(18)60716-1.Peer-Reviewed Original ResearchMeSH KeywordsBiological Transport, ActiveCloning, MolecularDNAElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueGene Expression RegulationGlycoprotein Hormones, alpha SubunitGlycoside HydrolasesGlycosylationHexosaminidasesHumansKineticsMannosyl-Glycoprotein Endo-beta-N-AcetylglucosaminidaseMembranesOligosaccharidesPituitary Hormones, AnteriorPlasmidsTunicamycinVesicular stomatitis Indiana virusViral Fusion ProteinsConceptsVesicular stomatitis virus glycoproteinAsparagine-linked glycansAnimal cellsAlpha subunitNovel cell surface proteinCarboxyl-terminal amino acidsGlycosylation inhibitor tunicamycinAbsence of glycosylationMembrane-anchored formCell surface proteinsSecond glycosylation siteHuman chorionic gonadotropin (hCG) alpha-subunitVirus glycoproteinEntire precursorCell surface expressionCytoplasmic domainGonadotropin alpha subunitHybrid proteinPlasma membraneGlycosylation sitesSecretory proteinsCellular membranesConformational changesCell surfaceAmino acidsA membrane-anchored form but not the secretory form of human chorionic gonadotropin-alpha chain acquires polylactosaminoglycan.
Fukuda M, Guan J, Rose J. A membrane-anchored form but not the secretory form of human chorionic gonadotropin-alpha chain acquires polylactosaminoglycan. Journal Of Biological Chemistry 1988, 263: 5314-5318. PMID: 2451668, DOI: 10.1016/s0021-9258(18)60717-3.Peer-Reviewed Original ResearchConceptsMembrane-anchored formHuman chorionic gonadotropin (hCG) alpha-subunitMembrane anchoringGonadotropin alpha subunitSecretory formVesicular stomatitis virus glycoproteinMonkey COS-1 cellsCOS-1 cellsMembrane-bound formCytoplasmic domainMembrane proteinsSecretory proteinsComplex-type oligosaccharidesSecretory glycoproteinsProteinDNAVirus glycoproteinSubunitsPolylactosaminoglycansCarbohydrate structuresGlycoproteinGlycansAnchoringAsnEnzyme