Featured Publications
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factorsDMA-tudor interaction modules control the specificity of in vivo condensates
Courchaine EM, Barentine AES, Straube K, Lee DR, Bewersdorf J, Neugebauer KM. DMA-tudor interaction modules control the specificity of in vivo condensates. Cell 2021, 184: 3612-3625.e17. PMID: 34115980, PMCID: PMC8402948, DOI: 10.1016/j.cell.2021.05.008.Peer-Reviewed Original ResearchImplementation of a 4Pi-SMS super-resolution microscope
Wang J, Allgeyer ES, Sirinakis G, Zhang Y, Hu K, Lessard MD, Li Y, Diekmann R, Phillips MA, Dobbie IM, Ries J, Booth MJ, Bewersdorf J. Implementation of a 4Pi-SMS super-resolution microscope. Nature Protocols 2020, 16: 677-727. PMID: 33328610, PMCID: PMC9118368, DOI: 10.1038/s41596-020-00428-7.Peer-Reviewed Original ResearchLight microscopy of proteins in their ultrastructural context
M’Saad O, Bewersdorf J. Light microscopy of proteins in their ultrastructural context. Nature Communications 2020, 11: 3850. PMID: 32737322, PMCID: PMC7395138, DOI: 10.1038/s41467-020-17523-8.Peer-Reviewed Original ResearchConceptsUltrastructural contextLocal protein densityConventional confocal microscopeCellular nanoarchitectureOptical contrastSpecific proteinsElectron microscopyFluorescence microscopyLight microscopyProteinBulk labelingProtein densityConfocal microscopeStandard light microscopyIntracellular spaceMicroscopyProteomeCellsNanoscaleMicroscopeNew principleDiscoveryLabelingPhysical expansionDensity
2024
VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1
Ugur B, Schueder F, Shin J, Hanna M, Wu Y, Leonzino M, Su M, McAdow A, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De Camilli P. VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1. Journal Of Cell Biology 2024, 223: e202311189. PMID: 39331042, PMCID: PMC11451052, DOI: 10.1083/jcb.202311189.Peer-Reviewed Original ResearchConceptsLipid transportGolgi complex proteinGolgi subcompartmentsGolgi membranesGolgi cisternaeProtein familyFunctional partnersGolgi complexKO cellsComplex proteinsFAM177A1GolgiVPS13BAdjacent membranesMutationsProteinCohen syndromeLipidOrthologsInteractorsBrefeldinMembraneOrganellesSubcompartmentsDevelopmental disordersNative architecture of a human GBP1 defense complex for cell-autonomous immunity to infection
Zhu S, Bradfield C, Maminska A, Park E, Kim B, Kumar P, Huang S, Kim M, Zhang Y, Bewersdorf J, MacMicking J. Native architecture of a human GBP1 defense complex for cell-autonomous immunity to infection. Science 2024, 383: eabm9903. PMID: 38422126, DOI: 10.1126/science.abm9903.Peer-Reviewed Original ResearchConceptsGuanylate-binding proteinsCaspase-4Surface of Gram-negative bacteriaGuanosine triphosphate hydrolysisImmunity to infectionInnate immunity to infectionCryo-electron tomographyGram-negative bacteriaImmunity proteinSignaling platformsMembrane insertionHuman cellsNative structureCombat infectionsLipopolysaccharide releaseGasdermin DExtended conformationLiving organismsProteinDefense complexCellsNative architectureGBP1BacteriaInfection
2022
A group approach to growing as a principal investigator
Greco V, Politi K, Eisenbarth S, Colón-Ramos D, Giraldez AJ, Bewersdorf J, Berg DN. A group approach to growing as a principal investigator. Current Biology 2022, 32: r498-r504. PMID: 35671717, DOI: 10.1016/j.cub.2022.04.082.Commentaries, Editorials and Letters
2020
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging
Zhang Y, Schroeder LK, Lessard MD, Kidd P, Chung J, Song Y, Benedetti L, Li Y, Ries J, Grimm JB, Lavis LD, De Camilli P, Rothman JE, Baddeley D, Bewersdorf J. Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Nature Methods 2020, 17: 225-231. PMID: 31907447, PMCID: PMC7028321, DOI: 10.1038/s41592-019-0676-4.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopeProtein complexesNanoscale resolutionSubcellular architectureMammalian cellsCellular organellesPlasma membraneGolgi apparatusEndoplasmic reticulumElectron microscopyThree-dimensional imagingFluorescence approachMulticolor imagingSpatial organizationLocalization precisionFluorescence imagingMolecular specificityFluorescence imagesMicroscopeFluorescent probeOrganellesReticulumMicroscopyImagingMembrane
2018
Assessing photodamage in live-cell STED microscopy
Kilian N, Goryaynov A, Lessard MD, Hooker G, Toomre D, Rothman JE, Bewersdorf J. Assessing photodamage in live-cell STED microscopy. Nature Methods 2018, 15: 755-756. PMID: 30275592, PMCID: PMC6915835, DOI: 10.1038/s41592-018-0145-5.Peer-Reviewed Original Research
2017
Biological Insight from Super-Resolution Microscopy: What We Can Learn from Localization-Based Images
Baddeley D, Bewersdorf J. Biological Insight from Super-Resolution Microscopy: What We Can Learn from Localization-Based Images. Annual Review Of Biochemistry 2017, 87: 1-25. PMID: 29272143, DOI: 10.1146/annurev-biochem-060815-014801.Peer-Reviewed Original Research
2016
Two-colour live-cell nanoscale imaging of intracellular targets
Bottanelli F, Kromann EB, Allgeyer ES, Erdmann RS, Wood Baguley S, Sirinakis G, Schepartz A, Baddeley D, Toomre DK, Rothman JE, Bewersdorf J. Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications 2016, 7: 10778. PMID: 26940217, PMCID: PMC4785223, DOI: 10.1038/ncomms10778.Peer-Reviewed Original Research